Transforming genes of carcinomas and neuroblastomas introduced into mouse fibroblasts

Abstract

We have previously demonstrated that DNA of mouse fibroblasts transformed by 3-methylcholanthrene (3-MC) induced foci of transformed cells when applied to monolayer cultures of NIH3T3 cells, which indicates that at least a part of this phenotype is encoded in DNA sequences. However, our conclusions were confined to the effects of DNAs of 3-MC-transformed mouse fibroblasts on recipient NIH3T3 cells, also of mouse fibroblast origin. To elucidate this phenomenon further, we have prepared DNAs from a series of mouse and non-mouse tumour lines of non-fibroblastic origin and investigated whether tumour transforming genes can act across tissue and species barriers to transform NIH3T3 cells. We find that DNAs obtained from human, rabbit and mouse bladder carcinoma lines, a lung carcinoma line and rat neuroblastoma and mouse glioma lines, are able to induce transformation of NIH3T3 cells on transfection.

Cellular heterogeneity in a tissue culture cell line derived from a human bladder carcinoma

“…Recent evidence for a molecular change in the DNA being responsible for the onset of neoplasia has been proposed using transfection techniques (Krontiris and Cooper, 1981;Parada et al, 1982;Perucho et al, 1981). Molecular DNA clones-oncogenes-which have been isolated from human bladder and other tumour lines have been shown to transform mouse fibroblast lines (Shih et al, 1981). The mouse cell lines used in these transfection studies are probably partially transformed, as one mouse fibroblast has been found to produce a low incidence of tumours in newborn mice (Shih et al, 1981).…”

“…Molecular DNA clones-oncogenes-which have been isolated from human bladder and other tumour lines have been shown to transform mouse fibroblast lines (Shih et al, 1981). The mouse cell lines used in these transfection studies are probably partially transformed, as one mouse fibroblast has been found to produce a low incidence of tumours in newborn mice (Shih et al, 1981). In view of this finding it may be that the oncogene influences the final stage of the transformation process i.e.…”

An optimum anti-melanoma response in mice immunized with fibroblasts transfected with DNA from mouse melanoma cells requires the expression of both syngeneic and allogeneic MHC-determinants

“…[23][24][25][26][27][28][29][30][31] These studies indicated that transfection of genomic DNA from one cell type to another resulted in integration of the exogenous DNA and stable expression of the trans-ferred genes. This was the case for genes specifying missing enzymes, as well as for genes specifying membraneassociated determinants.…”

Targeting the function of the HER2 oncogene in human cancer therapeutics

“…The importance of HER2 in cancer was realized in the early 1980s when a mutationally activated form of its rodent homolog neu was identified in a search for oncogenes in a carcinogeninduced rat tumorigenesis model (Shih et al, 1981). Its human homologue, HER2 was simultaneously cloned and found to be amplified in a breast cancer cell line (King et al, 1985).…”