Cellular heterogeneity in a tissue culture cell line derived from a human bladder carcinoma

Hastings, Richard P.; Franks, L. M.

doi:10.1038/bjc.1983.31

Cited by 38 publications

(18 citation statements)

References 23 publications

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“…Others have noted a similar correlation (Freedman & Shin, 1974). Colony forming efficiencies of the clones are within the ranges previously found for cell lines derived from bladder tumours (Hastings & Franks, 1983;Heckl et al, 1988) and bladder tumour cell suspensions (Kovnat et al, 1984. ) The cell line 647V, also derived from a human transitional cell carcinoma of the bladder, was shown to produce a substance which stimulated the growth of 647V cells (Messing et al, 1984).…”

Section: Growth In Vivosupporting

confidence: 61%

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Clonal analysis of a bladder cancer cell line: an experimental model of tumour heterogeneity

Brown

Russell²,

Philips³

et al. 1990

Br J Cancer

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Summary The continuous cell line UCRU BL 17CL was derived from a human invasive bladder cancer and expresses elements of transitional, squamous and glandular differentiation. Nine clones of this line were established by limit dilution and have been extensively characterised. Only six of these clones grew subcutaneously in nude mice. Of these, three have exhibited local invasion, each in one of five implanted mice. Although all xenografts expressed transitional, squamous and glandular elements, different histological subtypes predominated within each clone. Only clones which grew in nude mice formed colonies in semi-solid medium, and each responded differently to the influence of medium that had been conditioned by the growth of UCRU BL 17CL, suggesting the possible secretion of a growth factor by these cells. The DNA content and lectin binding profiles of the clones also reflected the heterogeneity of the line. UCRU BL 17CL and the nine clones provide a unique model for the study of tumour heterogeneity, progression and differentiation, and the potential autocrine regulation of growth of bladder cancer.In human bladder cancer, and in many other cancer types,

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Section: Growth In Vivosupporting

confidence: 61%

“…Although many of these lines have been extensively studied, there have been few reports of the isolation and characterisation of individual cell subpopulations within them (Hastings & Franks, 1983;Lin et al, 1985;Masters et al, 1986;Kovnat et al, 1988).…”

mentioning

confidence: 99%

Clonal analysis of a bladder cancer cell line: an experimental model of tumour heterogeneity

Brown

Russell²,

Philips³

et al. 1990

Br J Cancer

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“…JTC30 is a cell line established in our laboratory from a patient with welldifferentiated papillary bladder tumour (Kakuya et al, 1983). EJ was established from invasive, poorly differentiated urothelial carcinoma of the bladder (Evans et al, 1977;Hastings and Franks, 1983). T24 was derived from a primary lesion of grade 3 carcinoma of the bladder (Bubenik et al, 1973).…”

Section: Human Cell Linesmentioning

confidence: 99%

Impaired ΔNp63 expression associates with reduced β-catenin and aggressive phenotypes of urothelial neoplasms

et al. 2003

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p63, a homologue of the p53 gene, is considered to be essential for the normal development of stratified epithelia including urothelium. To examine possible roles of p63 in urothelial tumorigenesis, p63 expression was systematically examined in normal urothelium, low-grade papillary noninvasive (LPN) urothelial tumours, and high-grade or invasive carcinomas, using either an isoformnonspecific or a DN-isoform-specific antibody. Expression profiles of p63 were also analysed in cultured cells. Immunoreactivity with the two antibodies was virtually identical in tissue samples examined. Basal and intermediate cell layers of normal urothelium showed intense nuclear p63 immunostaining. This normal staining pattern was preserved in a majority of LPN tumours, whereas it was frequently impaired in high-grade or muscle-invasive carcinomas. At the mRNA level, DNp63 expression predominated over TAp63, and amounts of DNp63 mRNA correlated with p63 immunoreactivity, confirming that DNp63 accounts for p63 expressed in urothelial tissues. In cultured cells, DNp63 was also expressed in low-grade tumour cells as well as normal urothelial cells, but undetectable in high-grade aggressive carcinoma cells. Interestingly, impaired DNp63 expression significantly associated with reduced b-catenin expression that was possibly related to progression of urothelial neoplasms. Thus, impaired DNp63 expression characterises aggressive phenotypes of urothelial neoplasms.

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“…Cells and culture conditions. EJ-18-8D is a cloned cell line derivative of the original human EJ bladder carcinoma with an essentially dlploid chromosome complement (9) (Fig. 2).…”

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confidence: 99%

Molecular and physical arrangements of human DNA in HRAS1-selected, chromosome-mediated transfectants.

Porteous¹,

Morten²,

Cranston³

et al. 1986

Mol. Cell. Biol.

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We used mitotic chromosomes isolated from a human EJ bladder carcinoma cell line for morphological transformation of mouse C127 cells. These chromosome-mediated transformants were analyzed for cotransfer of markers syntenic with c-Ha-ras-1 on human chromosome 11. We also used cloned, dispersed human DNA repeats, in a general mapping strategy, to quantitate the amounts and molecular state of human DNA transferred along with the activated c-Ha-ras-1 gene. In situ hybridization was used to visualize the physical state of the transfected human chromatin. The combined use of these various techniques revealed the occurrence of both chromosomal and DNA rearrangements. However, our analysis also demonstrated that, in general, very substantial lengths of DNA are transferred intact. Closely linked markers are likely to cosegregate. Therefore, these transformants should be invaluable sources for the complete molecular cloning of isolated fragments of the short arm of human chromosome 11.CMGT is a long-established technique which enables variable subchromosomal lengths of DNA to be transferred efficiently to appropriate recipient cells (reviewed by McBride and Peterson [23]). Exploitation of this and other somatic cell techniques has been compromised by the limited availability of dominantly acting selection systems (23,31,39). We can now describe the use of a new class of dominant selectable markers in CMGT, the human oncogenes. Mitotic chromosomes derived from the human EJ bladder carcinoma cell line induced morphological transformation, anchorage-independent growth, and tumorigenicity in mouse C127 cells

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Cellular heterogeneity in a tissue culture cell line derived from a human bladder carcinoma

Cited by 38 publications

References 23 publications

Clonal analysis of a bladder cancer cell line: an experimental model of tumour heterogeneity

Clonal analysis of a bladder cancer cell line: an experimental model of tumour heterogeneity

Impaired ΔNp63 expression associates with reduced β-catenin and aggressive phenotypes of urothelial neoplasms

Molecular and physical arrangements of human DNA in HRAS1-selected, chromosome-mediated transfectants.

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