Clonal analysis of a bladder cancer cell line: an experimental model of tumour heterogeneity

Brown, Joanne; Russell, Pamela J.; Philips, Jeanette; Wotherspoon, John S.; Raghavan, Derek

doi:10.1038/bjc.1990.81

Cited by 56 publications

(39 citation statements)

References 16 publications

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“…and in vivo invasive abilities have been determined (Table II) (12,21). All lines examined possessed similar levels of KITENIN (Fig.…”

Section: ------------------------------------------------------------mentioning

confidence: 99%

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Expression of KITENIN, a KAI1/CD82 binding protein and metastasis enhancer, in bladder cancer cell lines: Relationship to KAI1/CD82 levels and invasive behaviour

Rowe

Jackson²

2006

Oncol Rep

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Abstract. KITENIN is a newly identified binding partner of the KAI1/CD82 metastasis suppressor. Recent studies using a mouse model of colon cancer, have suggested that KITENIN might be a metastasis enhancer whose functions are modulated by an interaction with KAI1/CD82. To begin exploration of the possible importance of KITENIN to human cancer, we examined KITENIN mRNA (by RT-PCR) and protein expression (by Western blotting) in a large series of bladder cancer cell lines, and then compared these levels to the expression of KAI1/CD82 and of previously determined in vitro invasive behaviour of these same cancer cell lines. We report that KITENIN was uniformly expressed in all cancer cell lines, but those lines in which KAI1/CD82 was not detected, had a higher in vitro invasive ability and altered actin organisation (as determined by fluorescence microscopy), than those lines in which KAI1/CD82 was present. Our data suggest that the relationship between KITENIN and KAI1/CD82 may be an important determinant of tumour cell behaviour.

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“…and in vivo invasive abilities have been determined (Table II) (12,21). All lines examined possessed similar levels of KITENIN (Fig.…”

Section: ------------------------------------------------------------mentioning

confidence: 99%

“…Invasive ability Relative in vitro KITENIN KAI1/CD82 (21) in nude mice in vivo (20) invasive ability (12) (±)…”

Section: Loss Of Actin Filaments In Representative Kitenin-positive Bmentioning

confidence: 99%

Expression of KITENIN, a KAI1/CD82 binding protein and metastasis enhancer, in bladder cancer cell lines: Relationship to KAI1/CD82 levels and invasive behaviour

Rowe

Jackson²

2006

Oncol Rep

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“…The DNA content and lectin binding profiles of the clones also reflects the heterogeneity of the line (Brown et al, 1990). Cytogenetic heterogeneity has also been demonstrated by comparative genomic hybridization (Voorter et al, 1995) and, in radiationinduced tumors, the presence of cytogenetically abnormal and unrelated clones has been assumed the result of heterogeneity of the tumor cell population (Fadl-Elmula et al, 1998).…”

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confidence: 92%

Molecular Evolution and Intratumor Heterogeneity by Topographic Compartments in Muscle-Invasive Transitional Cell Carcinoma of the Urinary Bladder

Díaz‐Cano

Blanes²,

Rubio³

et al. 2000

Laboratory Investigation

122

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SUMMARY:Superficial transitional cell carcinomas (TCC) of the urinary bladder have been shown to be monoclonal. However, no combined study of clonality and tumor suppressor genes (TSG) is available to date for muscle-invasive TCC. Forty-four muscle-invasive TCC of the urinary bladder selected from women were included in this study. Tumor cells located above and below the muscularis mucosa zone were systematically microdissected and used for DNA extraction. Hha-I digested and undigested samples were used to study the methylation pattern of androgen receptor alleles and undigested samples were used for microsatellite analysis of TSG (TP53, RB1, WT1, and NF1). Both loss of heterozygosity (LOH) and single nucleotide polymorphism (SNP) analyses were performed using optimized denaturing gradient gel electrophoresis. The expression of p53, pRB, and p21 WAF1 was assessed by immunohistochemistry. Appropriate controls were run in every case. All except two TCC showed a monoclonal pattern with the same allele inactivated in both compartments. Microsatellite analysis of TSG revealed the same LOH/SNP pattern in both tumor compartments in 30 cases (involving more than 1 TSG locus in 8) and genetic heterogeneity in 14 cases. From the latter group, 9 cases expressed more genetic changes in the deep compartment (involving TP53 gene in all cases, WT1 gene in 2, and NF1 in 1), whereas in 4 cases the superficial compartment showed more genetic changes (three involving NF1 and one involving both RB and TP53). No statistical difference in the immunoexpression was detected, although it tended to be higher in the superficial compartment than in the deep compartment. These concordant data in polymorphic DNA regions indicate that bladder-muscle-invasive TCC are monoclonal proliferations with homogeneous tumor cell selection. Heterogeneous tumor cell selection by topography defined two different genetic compartments: superficial, NF1-defective, and deep, TP53-defective. No differences in the immunohistochemical expression were observed, precluding a more extensive clinical application. (Lab Invest 2000, 80:279-289).

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“…We have derived a series of cloned lines from a recently established human bladder cancer cell line, which exhibits heterogeneity at several levels [34]. Of particular importance for the present study, the clones vary in their behaviour from nontumorigenic (NT), through tumorigenic (T) to invasive (I), when implanted in nude mice, and one of the invasive clones produces metastases when implanted orthotopically in nude rats [35].…”

Section: Introductionmentioning

confidence: 99%

Heterogeneity of in vitro radiosensitivity in human bladder cancer cells

Barnetson¹,

Banasiak²,

Fisher³

et al. 1999

Radiat. Oncol. Investig.

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Clonal analysis of a bladder cancer cell line: an experimental model of tumour heterogeneity

Cited by 56 publications

References 16 publications

Expression of KITENIN, a KAI1/CD82 binding protein and metastasis enhancer, in bladder cancer cell lines: Relationship to KAI1/CD82 levels and invasive behaviour

Expression of KITENIN, a KAI1/CD82 binding protein and metastasis enhancer, in bladder cancer cell lines: Relationship to KAI1/CD82 levels and invasive behaviour

Molecular Evolution and Intratumor Heterogeneity by Topographic Compartments in Muscle-Invasive Transitional Cell Carcinoma of the Urinary Bladder

Heterogeneity of in vitro radiosensitivity in human bladder cancer cells

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