Transformation of the oilseed crop Camelina sativa by Agrobacterium-mediated floral dip and simple large-scale screening of transformants

Liu, Xunjia; Brost, Jennifer M.; Hutcheon, Carolyn; Guilfoil, Robin; Wilson, Anna K.; Leung, Sharon Shui Yee; Shewmaker, Christine K.; Rooke, Suzanne; Nguyen, Thu Trang; Kiser, Jack; Rocher, Jay De

doi:10.1007/s11627-012-9459-7

Cited by 79 publications

(57 citation statements)

References 18 publications

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“…1a). The generated binary vector was introduced to C. sativa plants using the Agrobacterium-mediated floral dip method (Lu and Kang 2008;Liu et al 2012). After sowing the T1 transgenic C. sativa seeds, herbicide-resistant C. sativa seedlings were selected by spraying with BASTA solution (0.03 % v/v).…”

Section: Resultsmentioning

confidence: 99%

“…The cultivated Agrobacterium was centrifuged at 600 rpm for 8 min and then resuspended in transformation solution (5 % sucrose and 0.05 % silwet L-77) to a final concentration of OD 600 = 0.8. After bloomed flowers and siliques from 4-to-5-week-grown C. sativa plants were removed, the plants were transformed using the modified floral dip method (Lu and Kang 2008;Liu et al 2012). The buds were immersed into a transformation solution containing Agrobacterium harboring the SiW6:AtWRI1 three times with 1 week intervals.…”

Section: Construction Of a Binary Vectormentioning

confidence: 99%

“…C. sativa seeds contain 35-45 % storage oils per seed dry weight and its seed oils with high levels of unsaturated fatty acids are suitable for the production of jet fuels and biodiesel due to their physical properties such as lower melting point (Moser 2010(Moser , 2012Waraich et al 2013;Zubr 1997). In addition, it is known that C. sativa has a short life cycle (100-120 days) and can be relatively easily transformed via the Agrobacterium-mediated floral dip method (Lu and Kang 2008;Liu et al 2012;Zubr 1997). Therefore, while it is desirable to generate transgenic C. sativa with high levels of seed oil content, no evidence of this has yet been reported.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Overexpression of Arabidopsis WRI1 enhanced seed mass and storage oil content in Camelina sativa

Suh

2015

Plant Biotechnol Rep

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Section: Resultsmentioning

confidence: 99%

Section: Construction Of a Binary Vectormentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Overexpression of Arabidopsis WRI1 enhanced seed mass and storage oil content in Camelina sativa

Suh

2015

Plant Biotechnol Rep

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“…The pORE-O3 plasmids were introduced separately by electroporation into A. tumefaciens strain GV3101 with helper plasmid pMP90 (Koncz and Schell, 1986) and selected on LB agar medium containing 50 μg rifampicin ml -1 and 50 μg kanamycin ml -1 . A. tumefaciens GV3101 was grown in LB medium and used to transform C. sativa DH55 using the floral dip method (Liu et al, 2012). Transformed plants were selected by applying phosphinothricin (glufosinate) to 4 day-old seedlings.…”

Section: Methodsmentioning

confidence: 99%

Inoculation of Soil with Plant Growth Promoting Bacteria Producing 1-Aminocyclopropane-1-Carboxylate Deaminase or Expression of the Corresponding acdS Gene in Transgenic Plants Increases Salinity Tolerance in Camelina sativa

Heydarian

Gruber

et al. 2016

Front. Microbiol.

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Camelina sativa (camelina) is an oilseed crop touted for use on marginal lands; however, it is no more tolerant of soil salinity than traditional crops, such as canola. Plant growth-promoting bacteria (PGPB) that produce 1-aminocyclopropane-1-carboxylate deaminase (ACC deaminase) facilitate plant growth in the presence of abiotic stresses by reducing stress ethylene. Rhizospheric and endophytic PGPB and the corresponding acdS- mutants of the latter were examined for their ability to enhance tolerance to salt in camelina. Stimulation of growth and tolerance to salt was correlated with ACC deaminase production. Inoculation of soil with wild-type PGPB led to increased shoot length in the absence of salt, and increased seed production by approximately 30–50% under moderately saline conditions. The effect of ACC deaminase was further examined in transgenic camelina expressing a bacterial gene encoding ACC deaminase (acdS) under the regulation of the CaMV 35S promoter or the root-specific rolD promoter. Lines expressing acdS, in particular those using the rolD promoter, showed less decline in root length and weight, increased seed production, better seed quality and higher levels of seed oil production under salt stress. This study clearly demonstrates the potential benefit of using either PGPB that produce ACC deaminase or transgenic plants expressing the acdS gene under the control of a root-specific promoter to facilitate plant growth, seed production and seed quality on land that is not normally suitable for the majority of crops due to high salt content.

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“…Importantly, Camelina can be rapidly and easily transformed using Agrobacterium-mediated floral-dip methods [26,27]. The development of these methods makes Camelina transformation much simpler and quicker compared to the tissue-culture based approaches required for the transformation of other oilseed crops.…”

Section: Manipulating Lipid Metabolism In Camelinamentioning

confidence: 99%

Camelina sativa: An ideal platform for the metabolic engineering and field production of industrial lipids

Bansal

Durrett

2016

Biochimie

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Triacylglycerols (TAG) containing modified fatty acids with functionality beyond those found in commercially grown oil seed crops can be used as feedstocks for biofuels and bio-based materials. Over the years, advances have been made in transgenically engineering the production of various modified fatty acids in the model plant Arabidopsis thaliana. However, the inability to produce large quantities of transgenic seed has limited the functional testing of the modified oil. In contrast, the emerging oil seed crop Camelina sativa possesses important agronomic traits that recommend it as an ideal production platform for biofuels and industrial feedstocks. Camelina possesses low water and fertilizer requirements and is capable of yields comparable to other oil seed crops, particularly under stress conditions. Importantly, its relatively short growing season enables it to be grown as part of a double cropping system. In addition to these valuable agronomic features, Camelina is amenable to rapid metabolic engineering. The development of a simple and effective transformation method, combined with the availability of abundant transcriptomic and genomic data, has allowed the generation of transgenic Camelina lines capable of synthesizing high levels of unusual lipids. In some cases these levels have surpassed what was achieved in Arabidopsis. Further, the ability to use Camelina as a crop production system has allowed for the large scale growth of transgenic oil seed crops, enabling subsequent physical property testing. The application of new techniques such as genome editing will further increase the suitability of Camelina as an ideal platform for the production of biofuels and bio-materials.

show abstract

Transformation of the oilseed crop Camelina sativa by Agrobacterium-mediated floral dip and simple large-scale screening of transformants

Cited by 79 publications

References 18 publications

Overexpression of Arabidopsis WRI1 enhanced seed mass and storage oil content in Camelina sativa

Overexpression of Arabidopsis WRI1 enhanced seed mass and storage oil content in Camelina sativa

Inoculation of Soil with Plant Growth Promoting Bacteria Producing 1-Aminocyclopropane-1-Carboxylate Deaminase or Expression of the Corresponding acdS Gene in Transgenic Plants Increases Salinity Tolerance in Camelina sativa

Camelina sativa: An ideal platform for the metabolic engineering and field production of industrial lipids

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