Transformation of the Mesonephros into Epididymis in the Cock: Fate of Collecting and Secretory Tubule Antigens

Croisille, Y; Gumpel-Pinot, M; Gasc, Jean‐Marie

doi:10.1111/j.1432-0436.1978.tb00968.x

Cited by 6 publications

(2 citation statements)

References 14 publications

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“…During development of the metanephric kidney, the wolffian duct undergoes transformation into the efferent duct and proximal kidney tubule, but not the loop of Henle and distal tubule [32][33][34][35]. More than 95% of the testicular fluid is reabsorbed by the efferent duct [36].…”

Section: Discussionmentioning

confidence: 98%

Expression of Multiple Na+/H+ Exchanger Isoforms in Cultured Epithelial Cells from Rat Efferent Duct and Cauda Epididymidis1

Leung

Tse

Chew

et al. 2001

Biology of Reproduction

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Although earlier work has pointed to the presence of Na+/H+ exchangers (NHEs) in the rat epididymis, little is known about the regional distribution of various NHE isoforms and their functions. In the present work, expression of different isoforms of NHE in cultured epithelia of the efferent duct and cauda epdidymidis were studied. Reverse transcription-polymerase chain reaction revealed the presence of NHE1, NHE2, and NHE3, but not NHE4, message in both cultured epithelia. Western blot analysis detected the presence of NHE1 and NHE2 proteins in both cultured epithelia, but NHE3 protein was only detected in the cultured epithelial cells from the efferent duct. Immunohistochemical studies demonstrated that NHE2 was localized in the cytoplasm of the ciliated cells, whereas NHE3 was localized at the apical membrane of the principal cells of the efferent duct. The NHE activities in both cultured epithelia were inhibited by 10 microM HOE-694 (3-methylsulphonyl-4-piperidinobenzoyl guanidine methanesulphonate), a NHE1 inhibitor, by approximately 76%. The HOE-694-resistant NHE activities in the cultured epithelia of efferent duct and cauda epididymidis were completely inhibited by 20 microM S3226 (3-[2-(3-guanidino-2-methyl-3-oxo-propenyl)-5-methyl-phenyl]-N:-isopropylidene-2-methyl-acrylamide dihydrochloride), a NHE3 inhibitor, and 300 microM HOE-694 (a dose that can completely block NHE2), respectively. These results indicated that NHE1, NHE2, and NHE3 were expressed in the cultured epithelial cells of the efferent duct, whereas only NHE1 and NHE2 were expressed in the cultured epithelial cells of the cauda epididymidis. It is suggested that NHE1 may provide "housekeeping" functions in both epithelia, whereas NHE2 in the cauda epididymidis and NHE3 in the efferent duct may be involved in Na+ reabsorption and regulation of pH of the luminal fluid.

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Section: Discussionmentioning

confidence: 98%

Expression of Multiple Na+/H+ Exchanger Isoforms in Cultured Epithelial Cells from Rat Efferent Duct and Cauda Epididymidis1

Leung

Tse

Chew

et al. 2001

Biology of Reproduction

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show abstract

“…The terminus (T) is a narrow zone that enters the connective tissue capsule covering the caput epididymidis where the single ductule changes into the initial segment (IS) of the epididymis. Croisille et al, 1977Croisille et al, , 1978Linder, 1971). Furthermore, Marshall et al (1979) have found in rats exhibiting congenital renal agenesis and mesonephric anomaly (ACI rats) that a portion of the caput epididymis and the ductuli efferentes were present while all other Wolffian duct structures were missing.…”

Section: Developmentmentioning

confidence: 99%