Transformation of Solanum brevidens using Agrobacterium tumefaciens

Liu, T.-H. A.; Stephens, Loren C.; Hannapel, David J.

doi:10.1007/bf00193719

Cited by 20 publications

(8 citation statements)

References 22 publications

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“…andigena line 7540 as described by Liu et al (1995). These autotetraploid andigena plants strictly photoperiodic for tuberization were obtained from the Institut fü r Pflanzenbau und Pflanzenzü chtung (Braunchsweig, Germany).…”

Section: Plant Transformationmentioning

confidence: 99%

Interacting Transcription Factors from the Three-Amino Acid Loop Extension Superclass Regulate Tuber Formation

Chen

Rosin²,

Prat³

et al. 2003

Plant Physiology

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Using the yeast (Saccharomyces cerevisiae) two-hybrid system and a potato (Solanum tuberosum) KNOX protein, designated POTH1, as bait, we have identified seven distinct interacting proteins from a stolon library of potato. All seven cDNAs are members of the BEL1-like family of transcription factors. Among these proteins, there are at least four regions of high sequence conservation including the homeodomain, the proline-tyrosine-proline three-amino acid loop extension, the SKY box, and a 120-amino acid region upstream from the homeodomain. Through deletion analysis, we identified a proteinbinding domain present in the carboxy end of the KNOX domain of POTH1. The protein-binding domain in the BEL1 protein is located in the amino-terminal one-half of the 120-residue conserved region of the BELs. RNA-blot analysis showed differential patterns of RNA accumulation for the BELs in various potato organs. The level of StBEL5 mRNA increased in response to a short-day photoperiod in both leaves and stolons. Similar to sense mutants of POTH1, transgenic lines that overexpressed StBEL5 exhibited enhanced tuber formation even under noninductive conditions. Unlike POTH1 sense lines, however, these BEL lines did not exhibit the extreme leaf and stem morphology characteristic of KNOX overexpressers and displayed a more rapid rate of growth than control plants. Both StBEL5 and POTH1 sense lines exhibited an increase in cytokinin levels in shoot tips. StBEL5 lines also exhibited a decrease in the levels of GA 20-oxidase1 mRNA in stolon tips from long-day plants. Our results demonstrate an interaction between KNOX and BEL1-like transcription factors of potato that may potentially regulate processes of development.The primary developmental events of plants originate from apical meristems (Clark, 1997; Kerstetter and Hake, 1997). Many of these events are controlled at the molecular level by transcription factors (TFs). TFs are proteins that act as developmental switches by binding to the DNA (or to other proteins that bind to the DNA) of specific target genes to modulate their expression. An important family of TFs involved in regulating the developmental events in apical meristems is the knox (knotted-like homeobox) gene family (Reiser et al., 2000). Knox genes belong to the group of TFs known as the three-amino acid loop extension (TALE) superclass (Bü rglin, 1997). These TFs are distinguished by a very high level of sequence conservation in the DNA-binding region, designated the homeodomain, and consisting of three ␣-helices similar to the bacterial helix-loop-helix motif (Kerstetter et al., 1994). The third helix, the recognition helix, is involved in DNA binding (Mann and Chan, 1996). TALE TFs contain a TALE (Pro-Tyr-Pro) between helices I and II in the homeodomain that has been implicated in protein interactions (Passner et al., 1999). There are numerous TFs from plants and animals in the TALE superclass, and the two main groups in plants are the KNOX and BEL types (Bü rglin, 1997). Related genes in animal systems play an im...

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Section: Plant Transformationmentioning

confidence: 99%

Interacting Transcription Factors from the Three-Amino Acid Loop Extension Superclass Regulate Tuber Formation

Chen

Rosin²,

Prat³

et al. 2003

Plant Physiology

Self Cite

159

240

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“…Two potato cultivars, subsp. andigena and cv FL-1607, were transformed by the Agrobacterium tumefaciens (strain GV2260)-mediated leaf-disc transformation method (Liu et al, 1995). A total of 30 independent transgenic lines from potato subsp.…”

Section: S-poth1 Transformation Of Potato Plantsmentioning

confidence: 99%

Overexpression of a Knotted-Like Homeobox Gene of Potato Alters Vegetative Development by Decreasing Gibberellin Accumulation

Rosin¹,

Hart²,

et al. 2003

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Potato (Solanum tuberosum) homeobox 1 (POTH1) is a class I homeobox gene isolated from an early-stage tuber cDNA library. The RNA expression pattern of POTH1, unlike that of most other class I knotted-like homeobox genes, is widespread in the cells of both indeterminate and differentiated tissues. Using in situ hybridization, POTH1 transcripts were detected in meristematic cells, leaf primordia, and the vascular procambium of the young stem. Overexpression of POTH1 produced dwarf plants with altered leaf morphology. Leaves were reduced in size and displayed a "mouse-ear" phenotype. The mid-vein was less prominent, resulting in a palmate venation pattern. The overall plant height of overexpression lines was reduced due to a decrease in internode length. Levels of intermediates in the gibberellin (GA) biosynthetic pathway were altered, and the bioactive GA, GA 1 , was reduced by one-half in sense mutants. Accumulation of mRNA for GA 20-oxidase1, a key biosynthetic enzyme, decreased in overexpression lines. In vitro tuberization was enhanced under both short-and long-day photoperiods in several POTH1 overexpression lines. Sense lines produced more tubers at a faster rate than controls. These results imply that POTH1 mediates the development of potato by acting as a negative regulator of GA biosynthesis.Homeobox genes, a family of transcription factors highly conserved in animals, plants, and yeast (Chan et al., 1998), are implicated in the control of cell fate. The Antennapedia homeobox gene in fruitfly (Drosophila melanogaster), for example, specifies leg identity while inhibiting the formation of an antenna (Mann and Chan, 1996). Ectopic expression of the eyeless gene in the wing imaginal disc tissue of fruitfly embryos causes a normal eye to form on the wings (Halder et al., 1995). The first plant homeobox gene to be discovered was knotted1 (kn1) from maize (Zea mays; Vollbrecht et al., 1991). Dominant gain-offunction mutations of Kn1 formed knot-like structures along lateral veins. These knots were composed of cells that continued to divide rather than differentiate normally (Vollbrecht et al., 1991;Smith et al., 1992), indicating that kn1 is involved in regulating cell fate (Clark et al., 1996; Kerstetter et al., 1997; Chan et al., 1998).Knotted-like homeobox (knox) genes have been isolated from several plant species (for review, see Reiser et al., 2000) and can be divided into two classes based on expression patterns and sequence similarity (Kerstetter et al., 1994). Class I knox genes have high similarity to the kn1 homeodomain and generally have a meristem-specific mRNA expression pattern. Class II knox genes usually have a more widespread expression pattern. Knox genes are members of the three amino acid loop extension (TALE) superclass of homeobox genes (Bü rglin, 1997). The TALE superclass includes members from plants, animals, and fungi and is characterized by the addition of three amino acids, Pro-Tyr-Pro (PYP), between helices 1 and 2 of the homeodomain. Knox genes share conserved regions outside of...

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“…using Agrobacterium-mediated transformation (McCormick et al, 1986;Chyi and Phillips, 1987;Filatti et al, 1987;Davis et al, 1991;Joao and Brown, 1993;Hamza and Chupeau, 1993;Liu et al, 1995) and microprojectile bombardment (Van Eck et al, 1995) have been reported. In these transformation studies, most constructs contained a uidA reporter gene (Jefferson et al, 1986) driven by a CaMV 35S promoter allowing detection of transient and eventually stable expression of the uidA gene in transgenic plants, regardless of the stage of plant development or tissue localization.…”

Section: Introductionmentioning

confidence: 96%

Effect of an enhanced CaMV 35S promoter and a fruit-specific promoter on uida gene expression in transgenic tomato plants

Krasnyanski

Sandhu

Domier

et al. 2001

In Vitro Cell.Dev.Biol.-Plant

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Two different promoters, a cauliflower mosaic virus (CaMV) 35S promoter with a 5 H -untranslated leader sequence from alfalfa mosaic virus RNA4 (designated as CaMV 35S/AMV) and an E-8 fruit-ripening-specific promoter, were compared to evaluate their effects on expression of the uidA reporter gene in transgenic tomato plants. In order to generate sufficient numbers of transgenic tomato plants, both a reliable regeneration system and an efficient Agrobacterium transformation protocol were developed using 8-d-old cotyledons of tomato (Lycopersicon ecsulentum Mill. cv. Swifty Belle). Two sets of constructs, both derivatives of the binary vector pBI121, were used in transformation of tomato whereby the uidA gene was driven either by the CaMV 35S/AMV or the E-8 fruit-ripening-specific promoter. Southern blot hybridization confirmed the stable integration of the chimeric uidA gene into the tomato genome. Fruit and leaf tissues were collected from T 0 and T 1 plants, and assayed for b-glucuronidase (GUS) enzyme activity. As expected, both vegetative and fruit tissues of transgenic plants carrying the uidA gene under the control of CaMV 35S/AMV showed varying levels of GUS activity, while no expression was observed in vegetative tissues of transgenic plants carrying the uidA gene driven by the E-8 promoter. All fruits from transgenic plants produced with both sets of constructs displayed expression of the uidA gene. However, when this reporter gene was driven by the CaMV 35S/AMV, GUS activity levels were significantly higher than when it was driven by the E-8 fruit-specific promoter. The presence/absence of the uidA gene in T 1 plants segregated in a 3:1 Mendelian ratio.

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Transformation of Solanum brevidens using Agrobacterium tumefaciens

Cited by 20 publications

References 22 publications

Interacting Transcription Factors from the Three-Amino Acid Loop Extension Superclass Regulate Tuber Formation

Interacting Transcription Factors from the Three-Amino Acid Loop Extension Superclass Regulate Tuber Formation

Overexpression of a Knotted-Like Homeobox Gene of Potato Alters Vegetative Development by Decreasing Gibberellin Accumulation

Effect of an enhanced CaMV 35S promoter and a fruit-specific promoter on uida gene expression in transgenic tomato plants

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