Transformation of microflora during degradation of gaseous toluene in a biofilter detected using PCR-DGGE

Okunishi, Suguru; Morita, Yasutaka; Higuchi, Takashi; Maeda, Hiroto; Nishi, Katsuji

doi:10.1080/10962247.2012.672396

Cited by 16 publications

(3 citation statements)

References 38 publications

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“…The third anaerobic bacterium detected was the denitrifying Alicyclicphilus sp. This species can utilize aromatic hydrocarbons as carbon sources, which leads to a reduction in organic pollutant levels 42 . In addition to the anaerobically favorable conditions for Alicyclicphilus sp., the elevated nitrate and nitrite concentrations in fish ponds might explain its significantly high-representation in P-source samples.…”

Section: Discussionmentioning

confidence: 99%

Metabarcoding profiling of microbial diversity associated with trout fish farming

Mahmoud

Magdy

2021

Sci Rep

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Earthy and musty off-flavors are routinely observed in farmed trout worldwide. The microbial association to the production of those off-flavors was previously reported. The current manuscript aimed to catalog the microbial enrichment (eukaryotes and prokaryotes) in semi-intensive aquaculture freshwater sources that might influence the trout aquaculture quality production. The 16S rRNA and ITS metabarcoding analyses were applied on the inflow- and pond-water samples from trout farms previously recorded a malodor fish products and located alongside Moosach and Sempt Rivers in Bavaria province, Germany. The results showed that more than 99% of the detected prokaryotic OTUs (Operational Taxonomic Unit identification) were bacteria as of ~ 75.57% were Proteobacteria, and ~ 14.4% were Bacteroidetes. Meanwhile, 118 out of 233 of the eukaryotic OTUs were known species. Of these, ~ 45% were plant pathogens, and ~ 28% were mushroom/yeasts. Based on the comparative analysis between inflow- and pond-water samples, several pro- and eukaryotic microorganisms that affect the trout aquaculture water quality and industry have been detected, including the malodor-producing microorganisms, e.g., Cyanobacteria and Actinobacteria, along with fish infectious microorganisms, e.g., Chilodonella cyprinid, Metschnikowia bicuspidate. Additionally, the effect of the human- and industrial-related activities around the sampling area on the microbiota of the investigated farms were highlighted.

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Section: Discussionmentioning

confidence: 99%

Metabarcoding profiling of microbial diversity associated with trout fish farming

Mahmoud

Magdy

2021

Sci Rep

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“…Generally, biofiltration shows a good response when restarting from a period of shutdown (Singh, Rai, and Upadhhyay 2010); these data show that a higher supply rate of phosphorus provides the advantage of maintaining microbial activity during shutdown and of improving the efficiency of gaseous toluene removal during the restarting period. For example, a type of bacteria, Pseudomonas putida, a well-known toluene decomposer, is frequently observed in the biofiltration reactors used to treat gaseous VOC mixtures containing toluene (Okunishi et al 2012). A strain of P. putida is known to have a high capacity of accumulating phosphorus in the form of inorganic polyphosphates (poly-P) (Morohoshi et al 2003).…”

Section: Resultsmentioning

confidence: 99%

Influence of phosphorus and trace metals in biofilters treating gaseous VOCs using a novel irrigation system

Mansoori

Ando

Higuchi

2019

Journal of the Air & Waste Management Association

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Although the appropriate supply of nutrients has been extensively researched, more information is required on the effects of nutrients in treating gaseous volatile organic compounds (VOCs) in biofiltration. In this study, the effects of phosphorous and trace metals on gaseous toluene and methyl ethyl ketone (MEK) removal were investigated. The transfer of nutrients from the irrigation liquid to the packed bed, and the consumption and holding amount of nutrients in the packing material were observed during biofiltration. Under conditions of 20-24 s of empty bed residence time, MEK removal was 95% or more in all conditions of the biofiltration reactors, whereas toluene removal was affected by the operating conditions of the reactors. Consumption ratio of phosphorus to carbon was from 1.7 × 10 −4 to 1.1 × 10 −3 in the steady state of VOC removal under the conditions of this study. When gaseous VOC treatment was restarted after nine days of shutdown, a significant decline in toluene removal was observed by the reactor in which phosphorus supply was approximately one fifth of the amount in another reactor. Two types of irrigation systems, soaking and spraying, were compared and soaking irrigation achieved a more even distribution of nutrients held inside the packed bed. Soaking irrigation was expected to lead to higher VOC removal capacity by this distribution effect of nutrients, but toluene removal in the reactor with this irrigation was lower than that in the reactor with spraying irrigation. One of the possible reasons for this was the inhibition of nutrients transfer in the bottom part of the reactor. The trend of transfer in all ingredients from the irrigation liquid to the packed bed was synchronized on the whole; however, this transfer relatively tended to be high in nitrate and sodium and low in ammonium and phosphate.Implications: A major concern about using biofiltration systems to treat VOCs is the uncertainty regarding the appropriate nutrient supply to the filter bed to preserve microbial activity. This study showed that all the elements, except nitrogen, were retained sufficiently in the filter bed when a proper composition of nutrient solution was used for irrigation; however, phosphate addition may be needed when restarting a reactor from a prolonged period of shutdown. Distinct differences in the amount of transfer to the filter bed for different ingredients are probable, and may have to be taken into account when operating biofiltration reactors.

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“…the hydroxylase component of the T4-MO complex. tmoA has been widely used as a marker to evaluate bacterial community dynamics during bioremediation of BTEX-polluted soils and aquifers (Hendrickx et al 2006;Lee et al 2011;Okunishi et al 2012;Rakoczy et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Identification of novel toluene monooxygenase genes in a hydrocarbon-polluted sediment using sequence- and function-based screening of metagenomic libraries

Bouhajja

McGuire

Liles

et al. 2016

Appl Microbiol Biotechnol

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The microbial potential for toluene degradation within sediments from a tar oil-contaminated site in Flingern, Germany, was assessed using a metagenomic approach. High molecular weight environmental DNA from contaminated sediments was extracted, purified, and cloned into fosmid and BAC vectors and transformed into Escherichia coli. The fosmid library was screened by hybridization with a PCR amplicon of the α-subunit of the toluene 4-monooxygenase gene to identify genes and pathways encoding toluene degradation. Fourteen clones were recovered from the fosmid library, among which 13 were highly divergent from known tmoA genes and several had the closest relatives among Acinetobacter species. The BAC library was transferred to the heterologous hosts Cupriavidus metallidurans (phylum Proteobacteria) and Edaphobacter aggregans (phylum Acidobacteria). The resulting libraries were screened for expression of toluene degradation in the non-degradative hosts. From expression in C. metallidurans, three novel toluene monooxygenase-encoding operons were identified that were located on IncP1 plasmids. The E. aggregans-hosted BAC library led to the isolation of a cloned genetic locus putatively derived from an Acidobacteria taxon that contained genes involved in aerobic and anaerobic toluene degradation. These data suggest the important role of plasmids in the spread of toluene degradative capacity and indicate putative novel tmoA genes present in this hydrocarbon-polluted environment.

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Transformation of microflora during degradation of gaseous toluene in a biofilter detected using PCR-DGGE

Cited by 16 publications

References 38 publications

Metabarcoding profiling of microbial diversity associated with trout fish farming

Metabarcoding profiling of microbial diversity associated with trout fish farming

Influence of phosphorus and trace metals in biofilters treating gaseous VOCs using a novel irrigation system

Identification of novel toluene monooxygenase genes in a hydrocarbon-polluted sediment using sequence- and function-based screening of metagenomic libraries

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