Transformation of Metarhizium anisopliae with benomyl resistance and green fluorescent protein genes provides a tag for genetically engineered strains

Abstract

A plasmid, pBGFP, carrying green fluorescent protein (gfp) and benomyl-resistance genes was constructed and transformed into Metarhizium anisopliae. The transformants grew normally and GFP fluorescence was detected. No change was found in virulence for the transformants. Fluorescence was detected in hyphae from the haemolymph of the infected locust, and the benomyl-resistance was maintained. Results suggested that the two markers provided a useful tool for screening and monitoring the engineered strains even after infection.

Rhf1 gene is involved in the fruiting body production of Cordyceps militaris fungus

“…GFP protein was introduced into fungi, including M. anisopliae [1], Penicillium nordicum [40], and B. bassiana [24], as a selection marker. After ligating into the T-DNA, this protein was expressed in the hyphae of C. militaris transformants (Fig.…”

“…Higher yields of exopolysaccharide from C. militaris were obtained by UV mutagenesis [33]. The other factors, such as static culture or shake culture [8], light wave length [11], heavy metals and surfactants [9], salinity [55], culture temperature, humidity, air exchange, and plant hormones [1,9,11], were also important for C. militaris production and bioactive compound synthesis.…”

Mmc, a gene involved in microcycle conidiation of the entomopathogenic fungus Metarhizium anisopliae

“…1A) based on pBTM which was reported previously (Cao et al, 2007). pDPB contained a selectable marker, the bar gene (phosphinothricin acetyltransferase gene), polylinker, and two promotors in opposite direction (pgpd and ptrpc from A. nidulans).…”

Differentially-expressed glycoproteins in Locusta migratoria hemolymph infected with Metarhizium anisopliae

“…Conidia suspensions of CQMa102 were prepared in cottonseed oil at 1 · 10 7 spores/ml as previously described (Cao et al, 2007), and 5 ll were inoculated into the headthorax junction of locusts. Control locusts were inoculated with 5 ll cottonseed oil.…”