<i>Rhf1</i> gene is involved in the fruiting body production of <i>Cordyceps militaris</i> fungus

Jiang, Keqing; Han, Richou

doi:10.1007/s10295-015-1637-2

Cited by 8 publications

(8 citation statements)

References 63 publications

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“…The fruiting bodies of all C. militaris strains (CM10, ∆Cmfhp, and ∆Cmfhp-c) were cultured on rice medium according to a previously described method [14].…”

Section: Cultivation Of C Militaris Fruiting Bodiesmentioning

confidence: 99%

Cmfhp Gene Mediates Fruiting Body Development and Carotenoid Production in Cordyceps militaris

et al. 2020

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Cordyceps militaris fruiting bodies contain a variety of bioactive components that are beneficial to the human body. However, the low yield of fruiting bodies and the low carotenoid content in C. militaris have seriously hindered the development of the C. militaris industry. To elucidate the developmental mechanism of the fruiting bodies of C. militaris and the biosynthesis mechanism of carotenoids, the function of the flavohemoprotein-like Cmfhp gene of C. militaris was identified for the first time. The Cmfhp gene was knocked out by the split-marker method, and the targeted gene deletion mutant ΔCmfhp was obtained. An increased nitric oxide (NO) content, no fruiting body production, decreased carotenoid content, and reduced conidial production were found in the mutant ΔCmfhp. These characteristics were restored when the Cmfhp gene expression cassette was complemented into the ΔCmfhp strain by the Agrobacterium tumefaciens-mediated transformation method. Nonetheless, the Cmfhp gene had no significant effect on the mycelial growth rate of C. militaris. These results indicated that the Cmfhp gene regulated the biosynthesis of NO and carotenoids, the development of fruiting bodies, and the formation of conidia. These findings potentially pave the way to reveal the developmental mechanism of fruiting bodies and the biosynthesis mechanism of carotenoids in C. militaris.

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“…The fruiting bodies of all C. militaris strains (CM10, ∆Cmfhp, and ∆Cmfhp-c) were cultured on rice medium according to a previously described method [14].…”

Section: Cultivation Of C Militaris Fruiting Bodiesmentioning

confidence: 99%

Cmfhp Gene Mediates Fruiting Body Development and Carotenoid Production in Cordyceps militaris

et al. 2020

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“…But for the insertional mutant g38 in Rhf1 gene, the expression pattern of CmGpx was down-regulated. The Rhf1 gene was involved in the fruiting body production of C. militaris fungus and that silencing the Rhf1 gene could improve the formation and yields of fruiting bodies during the commercialization of this medicinal fungus [22]. The fruiting ability of degenerate C. militaris strain could be restored by overexpression an antioxidant glutathione peroxidase ( Gpx ) gene from Aspergillus nidulans to increase oxidative stress tolerance [28].…”

Section: Discussionmentioning

confidence: 99%

“…Two degenerate strains YN1-14 and YN2-7 were characterized by fluffy mycelia and reduced ability of stromata formation. Strain YN2-11 was a strain with normal fruiting body production, whereas strain g38 was an insertional mutant in Rhf1 gene with faster stromata development and higher yields [22]. All the strains were preserved in Guangdong Institute of Applied Biological Resources, Guangzhou, China.…”

Section: Methodsmentioning

confidence: 99%

Scratching Stimuli of Mycelia Influence Fruiting Body Production and ROS-Scavenging Gene Expression of Cordyceps militaris

Liu¹,

Qiu²,

Cao³

et al. 2018

Mycobiology

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The entomopathogenic fungus Cordyceps militaris is a valuable medicinal ascomycete, which degenerates frequently during subsequent culture. To avoid economic losses during industrialized production, scratching stimuli of mycelia was introduced to improve the fruiting body production. The present results indicated that higher yields and biological efficiency were obtained from two degenerate strains (YN1-14 and YN2-7) but not from g38 (an insertional mutant in Rhf1 gene with higher yields and shorter growth periods). Furthermore, the growth periods of the fruiting bodies were at least 5 days earlier when the mycelia were scratched before stromata differentiation. Three ROS-scavenging genes including Cu/Zn superoxide dismutase (CmSod1), Glutathione peroxidase (CmGpx), and Catalase A (CmCat A) were isolated and their expression profiles against scratching were determined in degenerate strain YN1-14 and mutant strain g38. At day 5 after scratching, the expression level of CmGpx significantly decreased for strain g38, but that of CmSod1 significantly increased for YN1-14. These results indicated that scratching is an effective way to promote fruiting body production of degenerate strain, which may be related at least with Rhf1 and active oxygen scavenging genes.

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“…The fruiting bodies of all strains (wild-type strain CM10, Δ Cmtns strain, and Δ Cmtns-c strain) were cultured on rice medium according to the method described in previous report (Jiang and Han, 2015). The fruiting bodies of C. militaris were vacuum freeze-dried, and then dried fruiting bodies were used for the determination of carotenoid content according to previously reported methods (Yang et al, 2014; Zhang et al, 2018).…”

Section: Methodsmentioning

confidence: 99%

Transcriptome Analysis of Cordyceps militaris Reveals Genes Associated With Carotenoid Synthesis and Identification of the Function of the Cmtns Gene

et al. 2019

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Cordyceps militaris, a valuable edible and medicinal fungus, has attracted increasing attention because of its various bioactive ingredients. However, the biosynthetic pathway of C. militaris carotenoids is still unknown due to lack of transcriptome information. To uncover genes related to the biosynthesis of C. militaris carotenoids, the transcriptomes of mycelia CM10_D cultured under dark conditions and mycelia CM10_L cultured under light exposure conditions were sequenced. Compared with mycelia CM10_D, 866 up-regulated genes and 856 down-regulated genes were found in mycelia CM10_L. Gene ontology (GO) analysis of differentially expressed genes (DEGs) indicated that DEGs were mainly classified into the “metabolic process,” “membrane,” and “catalytic activity” terms. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of DEGs suggested that DEGs were mainly enriched in “metabolic pathways,” “MAPK signaling pathway-yeast,” and “biosynthesis of secondary metabolites.” In addition, the carotenoid content of the Cmtns gene deletion mutant (ΔCmtns) was significantly lower than that of the wild-type C. militaris CM10, while the carotenoid content of the complementary strain (ΔCmtns-c) of the Cmtns gene was not significantly different from that of C. militaris CM10, suggesting that the Cmtns gene significantly affected the biosynthesis of carotenoids in C. militaris. These results potentially pave the way for revealing the biosynthetic pathway of carotenoids and improving carotenoids production in C. militaris.

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Rhf1 gene is involved in the fruiting body production of Cordyceps militaris fungus

Cited by 8 publications

References 63 publications

Cmfhp Gene Mediates Fruiting Body Development and Carotenoid Production in Cordyceps militaris

Cmfhp Gene Mediates Fruiting Body Development and Carotenoid Production in Cordyceps militaris

Scratching Stimuli of Mycelia Influence Fruiting Body Production and ROS-Scavenging Gene Expression of Cordyceps militaris

Transcriptome Analysis of Cordyceps militaris Reveals Genes Associated With Carotenoid Synthesis and Identification of the Function of the Cmtns Gene

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