Transformation of carnation by microprojectile bombardment

Zuker, Amir; Chang, Pi-Fang Linda; Ahroni, A.; Cheah, Kheng T.; Woodson, William R.; Bressan, Ray A.; Watad, A.A.; Hasegawa, Paul M.; Vainstein, Alexander

doi:10.1016/0304-4238(95)00817-9

Cited by 32 publications

(6 citation statements)

References 16 publications

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“…In some plant species decreasing shoot regeneration occurs with increasing donor plant age (Zuker et al, 1995). Kalanchoe¨follows this pattern: Even high level of TDZ could not induce shoot In some plant species juvenile leaves are morphologically and physiologically different from mature leaves.…”

Section: Other Conditions Influencing Shoot Regenerationmentioning

confidence: 97%

TDZ induces shoot regeneration in various Kalanchoë blossfeldiana Poelln. cultivars in the absence of auxin

Sanikhani¹,

Frello²,

Serek

2006

Plant Cell Tiss Organ Cult

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In order to optimize shoot regeneration in Kalanchoe¨blossfeldiana, leaf and internode explants of seven cultivars including one inter-specific were studied. The effects of various combinations of a-naphthalene acetic acid (NAA) (0, 0.57 M) and thidiazuron (TDZ) (0, 0.45, 4.5, 22.5, 67.5 lM) on MS medium were examined. In all cultivars shoot regeneration frequency and number of shoots per explant were enhanced by increasing TDZ concentration. Supplementing the media with NAA did not improve shoot regeneration. Maximum regeneration frequency and optimum concentration of TDZ for shoot regeneration depended significantly on the cultivar. Internode explants, but not leaf explants, of some cultivars, were able to produce adventitious shoots without treatment with growth regulator.

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Section: Other Conditions Influencing Shoot Regenerationmentioning

confidence: 97%

TDZ induces shoot regeneration in various Kalanchoë blossfeldiana Poelln. cultivars in the absence of auxin

Sanikhani¹,

Frello²,

Serek

2006

Plant Cell Tiss Organ Cult

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“…In the last 20 years, Agrobacterium tumefaciens -mediated transformation systems have been developed using different plant explants like leaves, petals, and stems Van Altvorst et al 1995, 1996Yantcheva et al 1997b ;Nontaswatsri et al 2004 ). Direct gene transfer by using microprojectile bombardment or with Agrobacterium was also successfully conducted (Zuker et al 1995(Zuker et al , 1999 in creating transgenic carnations with novel traits. The Moon Series composed of eight carnation varieties possessing soft to dark violet fl ower color was produced by Florigene.…”

Section: Genetic Transformation Of Carnationmentioning

confidence: 97%

Somatic Embryogenesis and Genetic Transformation of Carnation (Dianthus caryophyllus L.)

Iantcheva

2015

Somatic Embryogenesis in Ornamentals and Its Applications

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The chapter summarizes the progress of somatic embryogenesis and genetic transformation of carnation obtained during the last 40 years. Factors that determine the processes of indirect and direct somatic embryogenesis of this ornamental plant are described and discussed. The present chapter outlines the current results in the fi eld and focuses on the primary guidelines of genetic transformation of carnation. Future applications and intentions identifi ed by Bulgarian research group in this direction have also been highlighted.

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“…Compared to tobacco leaf discs, where target cells and responsive cells are limited to the edges around the leaf disc and therefore the frequency of transformation events is low, the subepidermal layers of thin cell layer explants have a high ability to regenerate buds (up to 800 buds per explant) or flowers (up to 50 flowers per explant) in a short space of time (10±12 d), and is a more suitable experimental model for the systematic study of (1) transformation events (via Agrobacterium, DNA uptake, or microprojectile-coated DNA), (2) the inheritance of the inserted trait, and (3) the expression of organspecific genes. Hypothetically, microprojectile bombardment may be used to target specific morphogenically competent cells that are located within tissues (Fromm et al, 1990;Reggiardo et al, 1991;Vasil et al, 1991;Zuker et al, 1995), and the TCL system provides a target tissue of specified cellular origin.…”

Section: Transformation Prospectsmentioning

confidence: 99%

Thin cell layer culture system in Lilium: Regeneration and transformation perspectives

Nhựt

Lệ

Silva

et al. 2001

In Vitro Cell.Dev.Biol.-Plant

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Lilies are one of the most valuable bulbous ornamental cut flower crops and are commercially propagated by conventional scaling, which results in varied bulb quality. This article demonstrates the effectiveness of using specific organ sources in conjunction with the thin cell layer (TCL) system to establish successful organogenic and somatic embryogenic pathways in Lilium longiflorum. TCLs derived from different explant sources, such as receptacle, leaf, young stem, stem node, bulblets, pseudo-bulblets, shoot and somatic embryos, respectively, can be manipulated to form different organs. Furthermore, the choice of plant growth regulator and medium additives such as activated charcoal and sucrose strongly affect the success of the process. This article further highlights that transient transgene expression derived from either particle bombardment or Agro-infection is both strong and repeatable, as a result of the direct exposure of competent cells to the gene introduction method, resulting in a reduction of escapes. The ability to manipulate and control organogenesis through a TCL system, coupled with repeatable, efficient transformation opens up the possibility of micropropagating members of this genus with new and available characteristics, producing superior plants of high quality.

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Transformation of carnation by microprojectile bombardment

Cited by 32 publications

References 16 publications

TDZ induces shoot regeneration in various Kalanchoë blossfeldiana Poelln. cultivars in the absence of auxin

TDZ induces shoot regeneration in various Kalanchoë blossfeldiana Poelln. cultivars in the absence of auxin

Somatic Embryogenesis and Genetic Transformation of Carnation (Dianthus caryophyllus L.)

Thin cell layer culture system in Lilium: Regeneration and transformation perspectives

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