Thin cell layer culture system in Lilium: Regeneration and transformation perspectives

Abstract

Lilies are one of the most valuable bulbous ornamental cut flower crops and are commercially propagated by conventional scaling, which results in varied bulb quality. This article demonstrates the effectiveness of using specific organ sources in conjunction with the thin cell layer (TCL) system to establish successful organogenic and somatic embryogenic pathways in Lilium longiflorum. TCLs derived from different explant sources, such as receptacle, leaf, young stem, stem node, bulblets, pseudo-bulblets, shoot and somatic embryos, respectively, can be manipulated to form different organs. Furthermore, the choice of plant growth regulator and medium additives such as activated charcoal and sucrose strongly affect the success of the process. This article further highlights that transient transgene expression derived from either particle bombardment or Agro-infection is both strong and repeatable, as a result of the direct exposure of competent cells to the gene introduction method, resulting in a reduction of escapes. The ability to manipulate and control organogenesis through a TCL system, coupled with repeatable, efficient transformation opens up the possibility of micropropagating members of this genus with new and available characteristics, producing superior plants of high quality.

Somatic embryogenesis and plant regeneration of Lilium ledebourii (Baker) Boiss., an endangered species

“…However, in terms of a perennial endangered plant species, a major disadvantage of using (pieces of) bulb scales as explant source is the destruction of the source plant. The lack of an efficient protocol for traditional asexual propagation of lily has prompted extensive studies on using the thin cell layer (TCL) method as both a tool and a solution to the use of bulb scales (Nhut et al 2001a). TCLs have been excised from different plant organs and successfully used as explants for SE and plant regeneration processes in L. longiflorum (Van Le et al 1999;Nhut et al 2001aNhut et al , b, 2002.…”

Standardization of an Effective Protocol for in vitro Culture of Lilium longiflorum Thunb cv. Pavia

“…Slow multiplication rate in conventional method is the main constraints in commercial expansion of these species. In vitro micropropagation provides an alternative for the rapid propagation of lilies (Nhut et al, 2001). In recent years, the tissue culture has proven to be most efficient for the mass propagation and multiplication of Lilium genus, as it helps in producing high quality plants (Kanchanapoom et al, 2011), and in maintaining germplasm of endangered and endemic species (Skoric et al, 2014).…”

The Effect of Naphthaleneacetic Acid and Two Cytokinins on Callus and Shoot Induction From Hypocotyls Thin Cell Layer Explants in Tomato (Lycopersicon Esculentum Mill)

“…The advantage of TCL culture is that thin cells layers are similar in physiological terms and can begin all morphogenesis patterns; however, only one pattern can be observed in each treatment (Kiem and Tran, 1981). In addition, direct organogenesis in most plants like Begonia tuberous (Nhut et al 2006), Lilium longiflorum (Nhut et al 2001), Citrus (Carimi et al 1999), Brassica napus (Ghnaya et al 2008) and Digitaria sanguinalis (Le et al 1997) has been reported using TCL. Since morphogenesis from hypocotyls TCL has not been reported in Lycopersicon esculentum Mill, in this research, morphogenesis (callus, shoot, root or somatic embryogenesis induction) containing various concentrations of NAA and two types of cytokinins (BAP and kin) were studied in the hypocotyl TCL of Lycopersicon esculentum Mill seeding.…”