Transformation-induced Mutations in Transgenic Plants: Analysis and Biosafety Implications

Wilson, Allison K.; Latham, Jonathan R.; Steinbrecher, Ricarda A.

doi:10.1080/02648725.2006.10648085

Cited by 75 publications

(49 citation statements)

References 137 publications

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“…Wilson et al 33 studied the effect of the plant transformation procedure on the plant genome. They analyzed the level and frequency of transformation-induced mutations in transgenic Poplar nigra plants produced by Agrobacterium-mediated transformation using RAPD analysis.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of genetically modified sugarcane lines carryingCry1AC gene using molecular marker techniques

Ismail¹

2013

GM Crops & Food

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Section: Resultsmentioning

confidence: 99%

Evaluation of genetically modified sugarcane lines carryingCry1AC gene using molecular marker techniques

Ismail¹

2013

GM Crops & Food

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“…In several instances Plant Mol Biol the molecular characterization of commercial transgenic crops showed the presence of vector fragments and modifications of the insertion site in the host genome. (Wilson et al 2006;Fitch et al 1992). Moreover Roundup Ready Ò Soybean 40-3-2 showed an intact copy of the CP4 EPSPS transgene followed by a 254 bp CP4 EPSPS fragment (Windels et al 2001).…”

Section: Discussionmentioning

confidence: 99%

Characterisation of 3′ transgene insertion site and derived mRNAs in MON810 YieldGard® maize

et al. 2008

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The construct inserted in YieldGard Ò MON810 maize, produced by Monsanto, contains the CaMV 35S promoter, the hsp70 intron of maize, the cryI(A)b gene for resistance to lepidopterans and the NOS terminator. In a previous work a truncation event at the 3 0 end of the cryI(A)b gene leading to the complete loss of the NOS terminator was demonstrated. The 3 0 maize genome junction region was isolated in the same experiment not showing any homology with known sequences. The aim of the experiments here reported was therefore to isolate and characterize a larger portion of the 3 0 integration junction from genomic DNA of two commercial MON810 maize lines. Specific primers were designed on the 3 0 integration junction sequence for the amplification of a 476 bp fragment downstream of the sequence previously detected. In silico analysis identified the whole isolated 3 0 genomic region as a gene putatively coding for the HECT E3 ubiquitin ligase. RT-PCR performed in this region produced cDNA variants of different length. In silico translation of these transcripts identified 2 and 18 putative additional aminoacids in different variants, all derived from the adjacent host genomic sequences, added to the truncated CRY1A protein. These putative recombinant proteins did not show homology with any known protein domains. Our data gave new insights on the genomic organization of MON810 in the YieldGard Ò maize and confirmed the previous suggestion that the integration in the genome of maize caused a complex recombination event without, apparently, interfering with the activity of the partial CRY1A endotoxin and both the vigor and yield of the YieldGard Ò maize.Keywords Cry-hect recombinant mRNAs Á 3 0 insertion site Á HECT protein ligase Á YieldGard Ò MON810 maize

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“…The first is that the process of genetic engineering itself creates unpredicted alterations, irrespective of which gene is transferred. This creates mutations in and around the insertion site and elsewhere [54]. The biotech industry confidently asserted that gene transfer from GM foods was not possible; the only human feeding study on GM foods later proved that it does take place.…”

Section: Gmos Are Inherently Unsafementioning

confidence: 99%

A Review on Impacts of Genetically Modified Food on Human Health

Verma¹,

Nanda²,

Singh³

et al. 2011

TONUTRAJ

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Abstract:Biotechnology offers a variety of potential benefits and risks. It has enhanced food production by making plants less vulnerable to drought, frost, insects, and viruses and by enabling plants to compete more effectively against weeds for soil nutrients. In a few cases, it has also improved the quality and nutrition of foods by altering their composition. However, the use of biotechnology has also raised concerns about its potential risks to the environment and people. For example, some people fear that common plant pests could develop resistance to the introduced pesticides in GM crops that were supposed to combat them. Genetic engineering provides a means to introduce genes into plants via mechanisms that are different in some respects from classical breeding. A number of commercialized, genetically engineered (GE) varieties, most notably canola, cotton, maize and soybean, were created using this technology, and at present the traits introduced are herbicide and/or pest tolerance. Gene technology enables the increase of production in plants, as well as the rise of resistance to pests, viruses, frost, etc. Gene transfer is used to modify the physical and chemical composition and nutritional value of food. Gene transfer in animals will play a part in boundless possibilities of improving qualitative and quantitative traits. The yield, carcass composition and meat characteristics the use of nutritive substances ? not sure what is being said here?, and resistance to diseases can be improved. On the other hand, negative effects of gene technology on animals, human, and environment should be considered. The present review article is the compilation of various studies that present both positive and negative impacts of genetically modified food on human health.

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Transformation-induced Mutations in Transgenic Plants: Analysis and Biosafety Implications

Cited by 75 publications

References 137 publications

Evaluation of genetically modified sugarcane lines carryingCry1AC gene using molecular marker techniques

Evaluation of genetically modified sugarcane lines carryingCry1AC gene using molecular marker techniques

Characterisation of 3′ transgene insertion site and derived mRNAs in MON810 YieldGard® maize

A Review on Impacts of Genetically Modified Food on Human Health

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