Evaluation of genetically modified sugarcane lines carryingCry1AC gene using molecular marker techniques

Ismail, Roba M.

doi:10.4161/gmcr.24299

Cited by 8 publications

(6 citation statements)

References 28 publications

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“…The exogenous cry1Ac gene was randomly inserted into various loci in the sugarcane nuclear genome. These results are also in agreement with Ismail’s [1] findings whereby independent transgenic sugarcane lines exhibited different copies of the cry1Ac gene, but harbored the same cassette of the foreign gene sequences and exhibited insect resistance [1]. …”

Section: Discussionsupporting

confidence: 92%

“…hybrids) is considered the most important crop for sugar production globally and is a valued raw material for the biofuel industry [1, 2]. Insect attack is a major issue in sugarcane cultivation, resulting in yield losses and sucrose content reduction [2].…”

Section: Introductionmentioning

confidence: 99%

“…There is an urgent need for borer resistance traits, and a series of studies that introduced the cry gene into sugarcane successfully obtained insect-resistant sugarcane lines [1, 4, 17–19]. However, only one case involving insect-resistant transgenic sugarcane has been approved for commercial planting in Brazil.…”

Section: Introductionmentioning

confidence: 99%

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Foreign cry1Ac gene integration and endogenous borer stress-related genes synergistically improve insect resistance in sugarcane

et al. 2018

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BackgroundSugarcane (Saccharum spp. hybrids) is considered the most globally important sugar-producing crop and raw material for biofuel. Insect attack is a major issue in sugarcane cultivation, resulting in yield losses and sucrose content reductions. Stem borer (Diatraea saccharalis F.) causes serious yield losses in sugarcane worldwide. However, insect-resistant germplasms for sugarcane are not available in any collections all over the world, and the molecular mechanism of insect resistance has not been elucidated. In this study, cry1Ac transgenic sugarcane lines were obtained and the biological characteristics and transgene dosage effect were investigated and a global exploration of gene expression by transcriptome analysis was performed.ResultsThe transgene copies of foreign cry1Ac were variable and random. The correlation between the cry1Ac protein and cry1Ac gene copies differed between the transgenic lines from FN15 and ROC22. The medium copy lines from FN15 showed a significant linear relationship, while ROC22 showed no definite dosage effect. The transgenic lines with medium copies of cry1Ac showed an elite phenotype. Transcriptome analysis by RNA sequencing indicated that up/down regulated differentially expressed genes were abundant among the cry1Ac sugarcane lines and the receptor variety. Foreign cry1Ac gene and endogenous borer stress-related genes may have a synergistic effect. Three lines, namely, A1, A5, and A6, were selected for their excellent stem borer resistance and phenotypic traits and are expected to be used directly as cultivars or crossing parents for sugarcane borer resistance breeding.ConclusionsCry1Ac gene integration dramatically improved sugarcane insect resistance. The elite transgenic offspring contained medium transgene copies. Foreign cry1Ac gene integration and endogenous borer stress-related genes may have a synergistic effect on sugarcane insect resistance improvement.Electronic supplementary materialThe online version of this article (10.1186/s12870-018-1536-6) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

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Foreign cry1Ac gene integration and endogenous borer stress-related genes synergistically improve insect resistance in sugarcane

et al. 2018

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“…Muhammad and Othman [19] studied banana somaclones with Random Amplified Polymorphic DNA (RAPD) and IRAP markers. They observed that RAPD markers were more polymorphic than IRAP markers [15]. IRAP was also recently used to study genetic diversity of cultivated flax (Linum usitatissimum L.) germplasm [20].…”

Section: Resultsmentioning

confidence: 99%

“…There are very limited reports on transgenic plants in terms of genomic variations [15]. In the present research, we aimed to analyse transgenic tobacco plants carrying herbicide-tolerance gene, dehE gene in terms of Tto1 retrotransposition using IRAP technique.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Transgenic Nicotiana tabacum with dehE Gene Using Transposon Based IRAP Markers

Kaya¹,

Yılmaz²,

Gözükırmızı³

et al. 2013

AJPS

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In the present study, five genetically modified herbicide tolerant Nicotiana tabacum cv. TAPM24 plants with a constructed vector pCAMBIA1301a carrying dehalogenase E (dehE) gene were compared with three non-transgenic controls using Tto1 retrotransposon specific IRAP markers. dehE gene was originally characterized in Rhizobium sp. and it produced an enzyme which degraded the Dalapon herbicide. IRAP protocol was applied on transgenic and non-transgenic plants to investigate the retrotransposon based genetic variation which may appear during transformation. Polymorphism rates were calculated as 0% -20% from IRAP-PCR products among all plant samples. These results show that transformation of tobacco plant with the dehE gene may cause Tto1 retrotransposon alterations appearing as different band profiles. The findings are expected to contribute to genetic engineering studies to obtain better results and also to understand how transposons contribute to features such as transgenesis. In our knowledge, this is one of the first experimental data of transgenic N. tabacum engineered with dehE gene originated Rhizobium sp. in terms of retrotranposon based variation.

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Sugarcane as a Novel Biofactory: Potentialities and Challenges

Gómez-Meriño

Trejo-Téllez

Sentíes-Herrera

2014

Biosystems Engineering: Biofactories for Food Production in the Century XXI

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Evaluation of genetically modified sugarcane lines carryingCry1AC gene using molecular marker techniques

Cited by 8 publications

References 28 publications

Foreign cry1Ac gene integration and endogenous borer stress-related genes synergistically improve insect resistance in sugarcane

Foreign cry1Ac gene integration and endogenous borer stress-related genes synergistically improve insect resistance in sugarcane

Evaluation of Transgenic <i>Nicotiana tabacum</i> with <i>deh</i>E Gene Using Transposon Based IRAP Markers

Sugarcane as a Novel Biofactory: Potentialities and Challenges

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Evaluation of genetically modified sugarcane lines carryingCry1AC gene using molecular marker techniques

Cited by 8 publications

References 28 publications

Foreign cry1Ac gene integration and endogenous borer stress-related genes synergistically improve insect resistance in sugarcane

Foreign cry1Ac gene integration and endogenous borer stress-related genes synergistically improve insect resistance in sugarcane

Evaluation of Transgenic &lt;i&gt;Nicotiana tabacum&lt;/i&gt; with &lt;i&gt;deh&lt;/i&gt;E Gene Using Transposon Based IRAP Markers

Sugarcane as a Novel Biofactory: Potentialities and Challenges

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Evaluation of Transgenic <i>Nicotiana tabacum</i> with <i>deh</i>E Gene Using Transposon Based IRAP Markers