Transformation in recurrent ovarian granulosa cell tumors: Ki67 (MIB-1) and p53 immunohistochemistry demonstrates a possible molecular basis for the poor histopathologic prediction of clinical behavior

Costa, Michael J.; Walls, Judy; Ames, Peter; Roth, Lawrence M.

doi:10.1016/s0046-8177(96)90069-6

Cited by 57 publications

(60 citation statements)

References 27 publications

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“…The clinical outcome was not found to be related to any statistical differences in immunoreactivity with any of the antibodies applied, and strong overexpression of p53 was not associated with a poor prognosis. Costa et al [17] also reported a curious lack of histopathologic predictors of recurrence and metastasis of ovarian GCT, particularly with regard to indicators of proliferative activity and overexpression of p53.…”

Section: Discussionmentioning

confidence: 99%

Granulosa Cell Tumor of the Ovary

Horny

Marx²,

Kröber³

et al. 1999

Gynecol Obstet Invest

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Background and Methods: Because the use of immunohistochemistry in the diagnosis of granulosa cell tumor (GCT) has not been fully explored, routinely processed (formalin-fixed, paraffin-embedded) tissue from 11 GCT, adult type, was investigated immunohistochemically (ABC method) with a broad spectrum of antibodies against various markers, including p53 and Ki-67. All of the tumors exhibited typical morphology, were limited to the ovary (stage I), and 7 cases followed a benign clinical course. Results: All the tumors exhibited strong expression of vimentin, but most other antigens (including smooth muscle actin) were expressed infrequently by a minority of tumor cells or not at all. Tumor cells in 9 GCT expressed inhibin A. All the tumors exhibited very low proliferative activity, fewer than 10% of the tumor cell nuclei being stained by the antibody MIB-1 (Ki-67 antigen). The antibody D07 revealed marked overexpression of p53 protein in only one tumor. Clinical outcome was not found to be related to immunophenotypic differences. Conclusions: The diagnosis of GCT should be based primarily on the typical morphology revealed by conventional stains, but additional immunohistochemical staining with a small panel of selected antibodies (for example, against keratin, vimentin, and inhibin A) may be helpful in a few cases. The very low proliferative activity and the lack of overexpression of p53 protein are consistent with the benign clinical behavior of the majority of GCT.

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Section: Discussionmentioning

confidence: 99%

Granulosa Cell Tumor of the Ovary

Horny

Marx²,

Kröber³

et al. 1999

Gynecol Obstet Invest

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“…Twenty-four hours later, cells terstained with hematoxylin for nuclear detection. 38 For were harvested, stained with propidium iodide and prepositive controls, neoplastic tissues known to be positive pared for flow cytometry by using CycleTest plus DNA for p53 were used. For negative controls, tumors injected reagent kit (Becton Dickinson, San Jose, CA, USA) followwith PBS or rAAV control were used.…”

Section: Methodsmentioning

confidence: 99%

Cancer gene therapy using a novel adeno-associated virus vector expressing human wild-type p53

Qazilbash

Xiao²,

Seth

et al. 1997

Gene Ther

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“…Il semble que le stade et la taille soient deux facteurs pronostiques indépendants. Le nombre de mitoses est également un facteur pronostique reconnu et il existe une relation inversement proportionnelle entre son taux élevé et la survie, quel que soit le stade considéré [28,31], ou seulement pour les stades II à IV [4,26]. Ainsi, dans la littérature, le nombre de mitoses est plus important dans les récidives précoces [31].…”

Section: Facteurs Pronostiquesunclassified

“…Le nombre de mitoses est également un facteur pronostique reconnu et il existe une relation inversement proportionnelle entre son taux élevé et la survie, quel que soit le stade considéré [28,31], ou seulement pour les stades II à IV [4,26]. Ainsi, dans la littérature, le nombre de mitoses est plus important dans les récidives précoces [31]. D'autres facteurs pronostiques sont retenus dans la littérature, même s'ils ne font pas l'objet d'un consensus, comme la rupture tumorale [4,26], qu'elle soit primitive ou secondaire à un geste chirurgical, les atypies nucléaires, corrélées [4,10,26] ou non [31] à une diminution de la survie selon les auteurs.…”

Section: Facteurs Pronostiquesunclassified