Transformation CodA Gene to Lily Plants by Agrobacterium tumefaciences Mediated

Abstract

Glycinebetaine (GB), an osmotic substance, could improve some stress tolerance in plants. CodA gene, originating from bacteria, could translate choline oxidase which stimulates the synthesis of GB in plants. To create lily lines resistant to heat, Belladonna lily and Yelloween lily had been transferred CodA gene through Agrobacterium tumefaciens. The bacteria harbored a binary vector carrying the hygromycin phosphotransferase, choline oxidase (CodA) and intron-containing β-glucuronidase (Gus) genes were co-cultivated with lily bulb scales slides. The result showed that most the bulb scales had developed into bulblets in a regulator-free growth medium, while some expressed the hygromycin-resistance, heat tolerance and Gus gene expression. Among them, one line demonstrated primarily the transcription level expression through reverse transcription polymerase chain reaction (RT-PCR). Moreover, they were tested with the accumulation of GB which was evident that the transferred line had four times of GB volumes higher than that of wild type. The original evidence could open a right approach to enhance stress tolerance in lily plants.