TRANSFORMASI GEN SUCROSE PHOSPHATE SYNTHASE (SoSPS1) MENGGUNAKAN Agrobacterium tumefaciens UNTUK MENINGKATKAN SINTESIS SUKROSA PADA TANAMAN TEBU (Saccharum officinarum L.)

Abstract

Sucrose phosphate synthase (SPS EC 2.3.1.14) plays an important role in partition of assimilated carbon in most plants. SPS catalyses the penultimate reaction in the pathway of sucrose synthesis, in which sucrose-6-phosphate (Suc6P) is synthesized from UDPglucose (UDPG) and fructose-6-P (Fru6P). To increase the capacity of sugarcane in sucrose synthesis, spindle leaves of sugarcane cv R579 were transformed with cDNA SoSPS1 from sugarcane under the control of constitutive promoter (35S CaMV) that constructed in pBI 121 (pKYS) using Agrobacterium tumefaciens. Based on PCR analysis, we have detected the existence of SPS transgene in some lines of transformed sugarcane, called line 4, 5, 6, and 7. The SPS transgene in transformed sugarcane could be expressed into translation level and increased the amount of leaves SPS protein, so the activity of leaves SPS was higher than wild type sugarcane as control. The transformed sugarcane line 4, 5, 6, and 7 showed 1.4–2.9 fold increases in SPS activity and 1,76–2,2 fold increases in leaves sucrose content. Increasing in SPS activity in transgenic sugarcane was coupled by the increase in invertase activity and ratio between sucrose and starch content.

The Effect of Edta-Se With Different Concentrations on Photosynthesis of Fragrant Rice (Oryza Sativa L.)

“…Normally, the accumulation of sucrose in leaves is controlled by synthase and decomposition enzyme related to the activity of key enzymes of sucrose metabolism including SPS, SS and sucrose invertase. SPS catalyzes sucrose synthesis reaction whilst SS had the duality of synthesizing and decomposing sucrose (Baxter et al, 2003;Tobias et al, 2008;Miswar et al, 2008). For example, SS mainly catalyzes sucrose synthesis in leaves; however, in grains it catalyzes sucrose degradation.…”

Transformasi Gen SoSPS1 dengan Menggunakan enhancer OsADH dan Eksplan Kalus Somatik Embriogenis pada Tanaman Tebu

“…Suc-6-P akan dihidrolisis oleh enzim Sucrose Phosphate Phosphatase (SPP) menjadi sukrosa. Semakin besar aktivitas enzim SPS, maka semakin besar substrat Suc-6-P yang dihasilkan, sehingga meningkatkan aktivitas enzim SPP dan dihasilkan sukrosa dalam jumlah besar (Miswar et al, 2007;Sawitri and Sugiharto, 2018).…”

Transformasi Genetik Tembakau dengan Gen Cold Shock Protein melalui Perantara Agrobacterium tumefaciens

“…N. tabacum (Mayo et al, 2006;Bhatti dan He, 2009) dan N. benthamiana (Anggraito, 2012) merupakan jenis tembakau yang sering digunakan dalam transformasi genetik. Pada jenis N. tabaccum seperti Samsun (Stanic et al, 1999), SRI (Bhatti dan He, 2009), Bright yellow (An, 1985), Xanthi (Su, 2012), dan Kasturi (Miswar, 2005), telah berhasil dilakukan transformasi melalui perantara A. tumefaciens. Daun muda (Jones, 1996;Su et al, 2012) dan suspensi sel (An, 1985;Mayo et al, 2006) merupakan eksplan yang telah berhasil diintroduksi gen asing.…”