Transferable plasmid-mediated antibiotic resistance in Listeria monocytogenes

Poyart, Claire; Carlier, C; Trieu-Cuot, Patrick; Courtieu, A.L.; Courvalin, Patrice

doi:10.1016/0140-6736(90)91447-i

Cited by 259 publications

(179 citation statements)

References 29 publications

Supporting

Mentioning

165

Contrasting

Unclassified

Order By: Relevance

“…In nine (41%) of these strains, tet(S) was associated with tet(M) and int-Tn, while in three (14%) strains it was also associated with tet(L). The finding of the tet(S) gene in strains of E. faecalis constitutes additional support for the notion that plasmid pIP811 from L. monocytogenes BM4210 originates in enterococci (22). None of the strains of Enterococcus and Streptococcus studied displayed homology with the tet(K), tetA(P), tetB(P), and tet(Q) probes.…”

Section: Methodsmentioning

confidence: 58%

“…Bacteriophage X DNA digested with PstI (A) and Raoul DNA molecular weight marker (R) served as internal standards. confers resistance to chloramphenicol, erythromycin, and streptomycin (22), and these isolates can be assigned to five groups according to their resistance to these antibiotics in addition to tetracycline-minocycline (Table 2). One group consisted of 10 strains that were resistant to chloramphenicol, erythromycin, and streptomycin and contained tet(S) alone or associated with tet(M) and int-Tn or with tet(M), int-Tn, and tet(L).…”

Section: Methodsmentioning

confidence: 99%

“…Genetic techniques. Mating on filters was performed as described earlier (22), and transfer frequencies were expressed as the number of transconjugants per donor CFU after the mating period. The antibiotics and concentrations used for selection of transcipients were as follows: fusidic acid, 10 ,ug/ml; rifampin, 20 ,ug/ml; and tetracycline, 5 ,ug/ml.…”

Section: Methodsmentioning

confidence: 99%

“…The Tet(S) protein shares 79 and 72% amino acid identities with Tet(M) and Tet(O), respectively, and is more distantly related to Tet(Q) (40% identity). In previous studies, it has been proposed that emergence of multiple-antibiotic resistance in L. monocytogenes BM4210 was secondary to acquisition of a replicon originating in the Enterococcus-Streptococcus genera (22). Demonstration of a direct exchange of genetic information between Enterococcusfaecalis and L. monocytogenes in the digestive tract of gnotobiotic mice (8) and detection in L. monocytogenes of tet(M) associated with int-Tn, the gene encoding the protein required for the movements of enterococcal conjugative transposons, constitute additional support for this suggestion (25).…”

mentioning

confidence: 99%

See 3 more Smart Citations

Presence of the Listeria tetracycline resistance gene tet(S) in Enterococcus faecalis

Charpentier

Gerbaud

Courvalin

1994

Antimicrob Agents Chemother

View full text Add to dashboard Cite

Two hundred thirty-eight tetracycline-and minocycline-resistant clinical isolates of Enterococcus and Streptococcus spp. were investigated by dot blot hybridization for the presence of nucleotide sequences related to tet(S) (first detected in Listeria monocytogenes BM4210), tet(K), tet(L), tet(M), tet(O), tet(P), and tet(Q) genes. The tet(S) determinant was found in 22 strains of Enterococcus faecalis, associated with tet(M) in 9 of these isolates and further associated with tet(L) in 3 of these strains. tet (M)

show abstract

Section: Methodsmentioning

confidence: 58%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Presence of the Listeria tetracycline resistance gene tet(S) in Enterococcus faecalis

Charpentier

Gerbaud

Courvalin

1994

Antimicrob Agents Chemother

View full text Add to dashboard Cite

show abstract

“…To overcome these problems, a wide range of synthetic antimicrobial agents (sodium benzoate, calcium benzoate, sorbate) has been used as food preservatives (Sherwin, 1990). However, antibiotic resistance has been described among Listeria spp., particularly L. monocytogenes strains isolated from food, the environment, or sporadic human listeriosis cases (Poyart-Salmeron, et al, 1990;Facinelli et al, 1991;Charpentier et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Synergistic Antibacterial Activity of Ecklonia cava (Phaeophyceae: Laminariales) against Listeria monocytogenes (Bacillales: Listeriaceae)

Nshimiyumukiza¹,

Kang²,

Kim³

et al. 2015

Fisheries and aquatic sciences

View full text Add to dashboard Cite

In an effort to discover alternative antimicrobials against Listeria monocytogenes, several marine algae were screened. The methanolic extract of Ecklonia cava exhibited the highest antibacterial activity against L. monocytogenes, with the ethyl acetate (EtOAc) soluble fraction of E. cava methanolic extract having a MIC value of 256 µg/mL and a MBC value of 512 µg/mL. The MIC values of streptomycin in combination with the EtOAc fraction were markedly reduced up to 64-fold, suggesting that the antibacterial activity of the antibiotic was restored when combined with the EtOAc fraction. The interaction between streptomycin and the EtOAc fraction was assessed by fractional inhibitory concentration (FIC) indices. The combination of streptomycin and the EtOAc fraction against L. monocytogenes resulted in ∑FIC min range of 0.141 to 0.266 and ∑FIC max of 0.531 for all strains. The median ∑FIC against L. monocytogenes strains ranged from 0.172 to 0.344. Thus, synergistic ranges of FIC <1 were observed for all combinations of streptomycin and the EtOAc fraction against L. monocytogenes strains. Indeed, the median ∑FIC of streptomycin-EtOAc fraction ranged from 0.172 to 0.344, suggesting a marked synergy.

show abstract

Listeria

McLauchlin¹,

Rees²

2015

Bergey's Manual of Systematics of Archaea and Bacteria

View full text Add to dashboard Cite

Lis.te'ri.a. N.L. fem. n. Listeria named after Lord Lister, English surgeon and pioneer of antisepsis. Firmicutes / “Bacilli” / Bacillales / “Listeriaceae” / Listeria Regular, short rods , 0.4–0.5 × 1–2 µm with parallel sides and blunt ends. Usually occur singly or in short chains. In older or rough cultures, filaments of ≥6 µm in length may develop. Gram‐positive with even staining, but some cells, especially in older cultures, lose their ability to retain the Gram strain. Not acid‐fast. Capsules not formed . Do not form spores. All species motile with peritrichous flagella when cultured <30°C. Aerobic and facultative anaerobic . Colonies (24–48 h) are 0.5–1.5 mm in diameter, round, translucent, low convex with a smooth surface and entire margin, non‐pigmented with a crystalline central appearance. May be sticky when removed from agar surfaces, usually emulsify easily, and may leave a slight impression on the agar surface after removal. Older cultures (3–7 d) are larger, 3–5 mm in diameter, have a more opaque appearance, sometimes with a sunken center: rough colonial forms may develop. In 0.25% (w/v) agar, 8% (w/v) gelatin and 1.0% (w/v) glucose semi‐solid medium, growth along the stab after 24 h at 37°C is followed by irregular, cloudy extensions into the medium. Growth spreads slowly through the entire medium. An umbrella‐like zone of maximal growth occurs 3–5 mm below the surface. Temperature limits of growth <0 to 45°C; optimal growth at 30–37°C. Do not survive heating at 60°C for 30 min . Growth occurs between pH 6 and pH 9. Growth occurs in nutrient broth supplemented with up to 10% (w/v) NaCl. Catalase‐positive, oxidase‐negative. Cytochromes produced. Homofermentative anaerobic catabolism of glucose results in production of L (+)‐ lactic acid, acetic acid plus other end products. Acid but no gas produced from other sugars. Methyl‐red‐positive, Voges–Proskauer‐positive . Exogenous citrate not utilized. Organic growth factors are required . Indole is not produced. Esculin and sodium hippurate are hydrolyzed. Urea is not hydrolyzed. Gelatin, casein, and milk are not hydrolyzed. DNA G + C content ( mol %): 36–42.5 ( T m ). Type species : Listeria monocytogenes Pirie 1940a.

show abstract

Transferable plasmid-mediated antibiotic resistance in Listeria monocytogenes

Cited by 259 publications

References 29 publications

Presence of the Listeria tetracycline resistance gene tet(S) in Enterococcus faecalis

Presence of the Listeria tetracycline resistance gene tet(S) in Enterococcus faecalis

Synergistic Antibacterial Activity of Ecklonia cava (Phaeophyceae: Laminariales) against Listeria monocytogenes (Bacillales: Listeriaceae)

Listeria

Contact Info

Product

Resources

About