Presence of the Listeria tetracycline resistance gene tet(S) in Enterococcus faecalis

Charpentier, Emmanuelle; Gerbaud, Guy; Courvalin, Patrice

doi:10.1128/aac.38.10.2330

Cited by 67 publications

(41 citation statements)

References 37 publications

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“…The different tet genes can have either the same mode of action (efflux or ribosomal protection), or different modes of action (efflux and ribosomal protection), just like the pathogenic and opportunistic species do (230). The carriage of multiple tet genes of different classes is commonly found in individual gram-positive isolates (37,236,266,267,307) and in Mycobacterium spp. and Streptomyces spp.…”

Section: Incidence Of Tetracycline Resistancementioning

confidence: 99%

Tetracycline Antibiotics: Mode of Action, Applications, Molecular Biology, and Epidemiology of Bacterial Resistance

Chopra

Roberts

2001

Microbiol Mol Biol Rev

3,545

2,643

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Tetracyclines were discovered in the 1940s and exhibited activity against a wide range of microorganisms including gram-positive and gram-negative bacteria, chlamydiae, mycoplasmas, rickettsiae, and protozoan parasites. They are inexpensive antibiotics, which have been used extensively in the prophlylaxis and therapy of human and animal infections and also at subtherapeutic levels in animal feed as growth promoters. The first tetracycline-resistant bacterium, Shigella dysenteriae, was isolated in 1953. Tetracycline resistance now occurs in an increasing number of pathogenic, opportunistic, and commensal bacteria. The presence of tetracycline-resistant pathogens limits the use of these agents in treatment of disease. Tetracycline resistance is often due to the acquisition of new genes, which code for energy-dependent efflux of tetracyclines or for a protein that protects bacterial ribosomes from the action of tetracyclines. Many of these genes are associated with mobile plasmids or transposons and can be distinguished from each other using molecular methods including DNA-DNA hybridization with oligonucleotide probes and DNA sequencing. A limited number of bacteria acquire resistance by mutations, which alter the permeability of the outer membrane porins and/or lipopolysaccharides in the outer membrane, change the regulation of innate efflux systems, or alter the 16S rRNA. New tetracycline derivatives are being examined, although their role in treatment is not clear. Changing the use of tetracyclines in human and animal health as well as in food production is needed if we are to continue to use this class of broad-spectrum antimicrobials through the present century

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Section: Incidence Of Tetracycline Resistancementioning

confidence: 99%

Tetracycline Antibiotics: Mode of Action, Applications, Molecular Biology, and Epidemiology of Bacterial Resistance

Chopra

Roberts

2001

Microbiol Mol Biol Rev

3,545

2,643

View full text Add to dashboard Cite

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“…The tet(S) gene is originally known from a Listeria monocytogenes plasmid (Charpentier et al, 1993) but subsequently, it has been found on the chromosome of Enterococcus faecalis (Charpentier et al, 1994;Francois et al, 1997) and on the conjugative plasmid of Lactococcus spp. (Perreten, 1997).…”

Section: Ribosomal Protection Determinantsmentioning

confidence: 99%

Tetracyclines in veterinary medicine and bacterial resistance to them

Michalova¹,

PNovotna²,

Schlegelová³

2004

Vet. Med.

117

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Since their discovery in 1945, tetracyclines have been used extensively in the therapy and prophylaxis of infectious diseases and as growth promoters. These wide applications have led to the equally fast spread of tetracycline resistant strains of gram-positive and gram-negative bacterial genera, including strains belonging to pathogenic as well as nonpathogenic species. Nonpathogenic bacteria could act as a reservoir of resistance determinants, which can be disseminated by horizontal transfer into pathogens. More than thirty different tetracycline resistance genes have been characterized. They encode two major mechanisms of resistance: 1 -active efflux of the antibiotic, and 2 -protection of ribosomes. Further mechanisms of tetracycline resistance include enzymatic inactivation of antibiotic, permeability barriers, mutations or multidrug transporter systems. Effective horizontal spread is favoured by the location of tetracycline resistance genes on mobile genetic elements such as plasmids and transposons. Their exchange, enhanced by the use of tetracyclines, is observed between bacteria of the same or different species and genera as well. Thus, questions of reevaluating and global reducing of tetracyclines in human and animal healthcare and food production are extensively discussed.

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“…Few more broad-host-range plasmids with ability of conjugative transfer of resistance to L monocytogenes such as pAMb1 of Enterococcus faecalis and pIP823 have been reported (37,75).…”

Section: Kratak Sadržajmentioning

confidence: 99%

“…Even though multi-resistant strains are not frequently isolated, evidence of their presence is available (204). Given the current wide use of antibiotics in food production and clinics, isolation of L. monocytogenes with reduced antibiotic susceptibility is expected to grow.Ever since the first antibiotic-resistant strain was described in France in1988 (176) Antibiotic resistance in L. monocytogenes is mostly due three mobile genetic elements: selftransferable plasmids, mobilizable plasmids, and conjugative transposons (37,40,97,176,177).Efflux pumps have also been reported to be present in Listeria (86). …”

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confidence: 99%

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Identification of the mechanisms of resistance and potential cross resistance of Listeria monocytogenes upon adaptation to different antimicrobials

Rakić-Martinez¹

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ACKNOWLEDGMENTSI would like to thank Dr. Vera Katić for being my mentor, not only through PhD studies, but in my undergraduate years as well. Her objectivity, articulate thinking and dedication to work inspired me to keep moving forward even after long, tiresome days in the lab. I will forever appreciate Dr. Katic's encouragement to pursue personal happiness and her support in important life decisions.I am forever grateful to Dr. Sophia Kathariou for accepting me in her lab and making me feel at home while far from it. Her incredible ability to find the best solutions when things were not looking promising and open completely new horizons along with her creativity and knowledge made science so attractive. Looking at all the great, bright and skilful young people pursuing their dreams under Dr. Kathariou's guidance makes me very proud for being a part of it.My appreciation also goes to Dr. Lepšanović and Dr. Mišić for accepting to be members of the committee. I am thankful to all my lab mates and to Ms. Robin Siletzky for her solid support throughout my work. A special thanks to Dr. Vikrant Dutta for his patience, willingness to help and his much admired teaching skills.Lastly and foremost I would like to thank my family. To my dear parents Ljubo and Zora whose unconditional love, support and presence whenever I needed them have been and will always be my motive to be better. To my big brother Zoran, who has been, and is my role model, but still owes me German lessons. Finally, I want to thank my boys, my beloved husband Sergio and my precious son Gregory, who have been an endless source of energy, support and love. Thanks for being my wingmen guys! ABSTRACTAbility to contaminate ready-to-eat, highly processed, cold-stored food products, relatively high fatality rate and environmental adaptations define .Listeria monocytogenes as a challenging foodborne pathogen of high public health significance. Listeria's adaptations and prolonged persistence in food processing facilities are a key link to contamination of foods.In recent years, bacterial multidrug resistance has been recognized as an emerging health challenge. Exposure to antimicrobials (antibiotics, disinfectants or environmentally ubiquitous heavy metals), may provide selective pressures for resistance with accompanying cross-resistance and increase in environmental fitness.In this work I addressed some of the possible mechanisms of adaptations that may contribute to persistence of L. monocytogenes in the environment as well as to survival and growth in different foods. This includes adaptations to heavy metals such as cadmium, disinfectants such as benzalkonium chloride (BC), selected antibiotics (e.g. the fluoroquinolone ciprofloxacin) and other toxic compounds (e.g. tetraphenyl-phosponium, often used as an anti-carcinogenic and efflux pump substrate). In order to characterize the impact of multidrug resistance on survival and growth of L. monocytogenes in different foods, we tested mutants adapted to higher concentrations of antibiotic ciprofloxacin and ...

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Presence of the Listeria tetracycline resistance gene tet(S) in Enterococcus faecalis

Cited by 67 publications

References 37 publications

Tetracycline Antibiotics: Mode of Action, Applications, Molecular Biology, and Epidemiology of Bacterial Resistance

Tetracycline Antibiotics: Mode of Action, Applications, Molecular Biology, and Epidemiology of Bacterial Resistance

Tetracyclines in veterinary medicine and bacterial resistance to them

Identification of the mechanisms of resistance and potential cross resistance of Listeria monocytogenes upon adaptation to different antimicrobials

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