Transferability of molecular markers from major legumes to Lathyrus spp. for their application in mapping and diversity studies

Almeida, Nuno Felipe; Leitão, Susana T.; Caminero, C.; Torres, A. M.; Rubiales, Diego; Patto, María Carlota Vaz

doi:10.1007/s11033-013-2860-4

Cited by 35 publications

(25 citation statements)

References 64 publications

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“…A total of 935 molecular markers (767 SNPs, 163 E-SSRs and 5 intron targeted amplified polymorphism (ITAP) markers from a previous study 11 , were screened in the parental genotypes of the F5 RIL population for mapping purposes. From these, 307 polymorphic loci were successfully mapped, namely, 189 SNPs, 113 E-SSRs and 5 ITAPs.…”

Section: Resultsmentioning

confidence: 99%

“…Previous studies on pathosystems involving L. cicera described its response to rust 11 , powdery mildew 7 , broomrape 9,21 and bacterial blight 8 at the physiological level. The present study contributes to progress in L. cicera research and breeding in several respects.…”

Section: Discussionmentioning

confidence: 99%

“…Eventually these may also be used, by cross-amplification 11 , for genetic mapping in L. sativus . The many other genic SNPs identified here may also be converted into molecular markers.…”

Section: Discussionmentioning

confidence: 99%

“…In both, L. cicera and L. sativus , resistant genotypes restrict the formation of haustoria resulting in a high percentage of early aborted fungal colonies, a decreased number of haustoria per colony and reduced intercellular growth of infection hyphae compared to susceptible genotypes from the same species 6,11,13 . Still, L. sativus is generally more resistant than L. cicera .…”

Section: Discussionmentioning

confidence: 99%

“…However, molecular tools from related species were useful in this legume. Actually, 138 molecular markers of different types previously developed for other legume species, were successfully cross-amplified in L. cicera 11 .…”

Section: Introductionmentioning

confidence: 99%

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First genetic linkage map of Lathyrus cicera based on RNA sequencing-derived markers: Key tool for genetic mapping of disease resistance

et al. 2018

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The Lathyrus cicera transcriptome was analysed in response to rust (Uromyces pisi) infection to develop novel molecular breeding tools with potential for genetic mapping of resistance in this robust orphan legume species. One RNA-seq library each was generated from control and rust-inoculated leaves from two L. cicera genotypes with contrasting quantitative resistance, de novo assembled into contigs and sequence polymorphisms were identified. In toto, 19,224 SNPs differentiate the susceptible from the partially resistant genotype’s transcriptome. In addition, we developed and tested 341 expressed E-SSR markers from the contigs, of which 60.7% varied between the two L. cicera genotypes. A first L. cicera linkage map was created using part of the developed markers in a RIL population from the cross of the two genotypes. This map contains 307 markers, covered 724.2 cM and is organised in 7 major and 2 minor linkage groups, with an average mapping interval of 2.4 cM. The genic markers also enabled us to compare their position in L. cicera map with the physical position of the same markers mapped on Medicago truncatula genome, highlighting a high macrosyntenic conservation between both species. This study provides a large new set of genic polymorphic molecular markers with potential for mapping rust resistances. It represents the first step towards genomics-assisted precision breeding in L. cicera.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…Eventually these may also be used, by cross-amplification 11 , for genetic mapping in L. sativus . The many other genic SNPs identified here may also be converted into molecular markers.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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First genetic linkage map of Lathyrus cicera based on RNA sequencing-derived markers: Key tool for genetic mapping of disease resistance

et al. 2018

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Grain Legumes

2015

Handbook of Plant Breeding

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The field of plant breeding covers a broad range of different species and categories of plants. While there are many techniques and issues that are similar across these species, many more are unique to each genus.The Handbook of Plant Breeding is organized by major crop categories and includes the most up-to-date molecular techniques being used. It will serve as a resource for plant breeding laboratories in both the university and industrial setting.More information about this series at

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