Transferability and characterization of microsatellite markers from Byrsonima cydoniifolia A. Juss. (MALPIGHIACEAE) in seven related taxa from Cerrado biome reveal genetic relationships

Abstract

Byrsonima Rich. is one of the largest genera of the Malpighiaceae family, with 97 species occurrence in Brazil. In this study, 17 microsatellite markers previously developed and characterized in Byrsonima cydoniifolia A. Juss. were tested for seven related taxa. All species tested here are native to Brazil, and of these species four are endemic. Cross-ampli cation as successfully optimized with high e ciency for all species. Microsatellite markers panels ranged from 11 (64,8%) transfered markers in B. viminifolia to 6 (35.2%) in B. umbellata. All loci were genotyped for 16 individuals of each species, except for B. viminifolia (14 individuals), then the polymorphic loci were characterized. The total number of alleles per locus across tested species ranged from 5 (B. linearifolia) to 8 (B. subterranea) alleles. B. umbellata showed lower values of observed and expected heterozygosity (Ho = 0.312; He = 0.436) and B. subterranea presented the highest values (Ho = 0.687; He = 0.778). A greater number of microsatellite markers should be developed for B. umbellata, once that the markers set transferred is reasonably informative, due to the greatest genetic divergence between species. The microsatellite marker panels transferred to the species B. intermedia, B. verbascifolia, B. laxi ora, B. subterranea, B. viminifolia and B. linearifolia are very informative, with a high combined probability of exclusion of paternity (Q ≥ 0.976) and the low combined probability of identity (I ≤ 9.91x10 − 6 ), potentially suitable for future genetic-population studies, supporting strategies for maintaining the genetic diversity and for exploration of Byrsonima species as genetic resources.

Genetic Diversity Study on Geographical Populations of the Multipurpose Species Elsholtzia stauntonii Using Transferable Microsatellite Markers

“…Cross-taxa transferability enables the development of microsatellite markers, based on markers from related species, at low cost ( Kalia et al, 2011 ; Bernardes et al, 2021 ). In the present study, the transferability observed (9.05%) was similar to the average of approximately 10% reported in cross-genera transferability studies of eudicots between 1997 and mid-2006 ( Barbará et al, 2007 ).…”

Transcriptomic Insights and the Development of Microsatellite Markers to Assess Genetic Diversity in the Broodstock Management of Litopenaeus stylirostris

“…Microsatellite markers are simple sequence repeats of highly polymorphic and codominant DNA motifs (n could range from 8 to 50) that are widely distributed throughout the genomes of eukaryotes and are widely used for different population genetic studies in many different species, such as genetic diversity [ 27 , 28 , 29 ], population structure, parentage determination [ 30 , 31 , 32 ], and constructing genetic linkage maps [ 33 , 34 , 35 , 36 , 37 ], animal identification, meat traceability [ 38 , 39 ] and animal breeding [ 40 , 41 ]. Studies on related aquatic species with highly duplicated genomes have demonstrated that microsatellites derived from transcriptomes are significantly more effective than those developed from the genome using traditional methods [ 42 ].…”