Transfer RNA‑derived fragment tRF‑28‑QSZ34KRQ590K in plasma exosomes may be a potential biomarker for atopic dermatitis in pediatric patients

Li, Meng; Jiang, Long; Chen, Jian; Ren, Hongjin; Gao, Zhiqin; Wu, Fei; Wen, Yiyang; Yang, Lianjuan

doi:10.3892/etm.2021.9920

Cited by 11 publications

(7 citation statements)

References 23 publications

(25 reference statements)

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“…32 The effect size between preeclampsia and normal is consistent with other studies of differential 5ʹ-tRF expression in disease. 33 We corroborated discoveries in perfusion data by finding increased STB-EV 5ʹ-tRF-Glu-CTC in preeclampsia plasma compared with normotensive controls.…”

Section: Discussionsupporting

confidence: 86%

Differential 5′-tRNA Fragment Expression in Circulating Preeclampsia Syncytiotrophoblast Vesicles Drives Macrophage Inflammation

Cooke,

Jiang,

et al. 2024

Hypertension

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BACKGROUND: The relationship between placental pathology and the maternal syndrome of preeclampsia is incompletely characterized. Mismatch between placental nutrient supply and fetal demands induces stress in the syncytiotrophoblast, the layer of placenta in direct contact with maternal blood. Such stress alters the content and increases the release of syncytiotrophoblast extracellular vesicles (STB-EVs) into the maternal circulation. We have previously shown 5′-tRNA fragments (5′-tRFs) constitute the majority of small RNA in STB-EVs in healthy pregnancy. 5′-tRFs are produced in response to stress. We hypothesized STB-EV 5′-tRF release might change in preeclampsia. METHODS: We perfused placentas from 8 women with early-onset preeclampsia and 6 controls, comparing small RNA expression in STB-EVs. We used membrane-affinity columns to isolate maternal plasma vesicles and investigate placental 5′-tRFs in vivo. We quantified 5′-tRFs from circulating STB-EVs using a placental alkaline phosphatase immunoassay. 5′-tRFs and scrambled RNA controls were added to monocyte, macrophage and endothelial cells in culture to investigate transcriptional responses. RESULTS: 5′-tRFs constitute the majority of small RNA in STB-EVs from both preeclampsia and normal pregnancies. More than 900 small RNA fragments are differentially expressed in preeclampsia STB-EVs. Preeclampsia-dysregulated 5′-tRFs are detectable in maternal plasma, where we identified a placentally derived load. 5′-tRF-Glu-CTC, the most abundant preeclampsia-upregulated 5′-tRF in perfusion STB-EVs, is also increased in preeclampsia STB-EVs from maternal plasma. 5′-tRF-Glu-CTC induced inflammation in macrophages but not monocytes. The conditioned media from 5′-tRF-Glu-CTC-activated macrophages reduced eNOS (endothelial NO synthase) expression in endothelial cells. CONCLUSIONS: Increased release of syncytiotrophoblast-derived vesicle-bound 5′-tRF-Glu-CTC contributes to preeclampsia pathophysiology.

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Section: Discussionsupporting

confidence: 86%

Differential 5′-tRNA Fragment Expression in Circulating Preeclampsia Syncytiotrophoblast Vesicles Drives Macrophage Inflammation

Cooke,

Jiang,

et al. 2024

Hypertension

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“…Meng et al [ 73 ] for the first time attempted to elucidate the role of exosomal tRFs in paediatric patients with atopic dermatitis. Using plasma samples from three atopic dermatitis patients and three healthy controls, the authors revealed an impressive 135 exosomal tRFs which are differentially expressed.…”

Section: Resultsmentioning

confidence: 99%

The Clinical Significance of Transfer RNAs Present in Extracellular Vesicles

Liu

Yang

Asim

et al. 2022

IJMS

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Extracellular vesicles (EVs) are important for intercellular signalling in multi-cellular organisms. However, the role of mature transfer RNAs (tRNAs) and tRNA fragments in EVs has yet to be characterised. This systematic review aimed to identify up-to-date literature on tRNAs present within human EVs and explores their potential clinical significance in health and disease. A comprehensive and systematic literature search was performed, and the study was conducted in accordance with PRISMA guidelines. Electronic databases MEDLINE and EMBASE were searched up until 1 January 2022. From 685 papers, 60 studies were identified for analysis. The majority of papers reviewed focussed on the role of EV tRNAs in cancers (31.7%), with numerous other conditions represented. Blood and cell lines were the most common EV sources, representing 85.9% of protocols used. EV isolation methods included most known methods, precipitation being the most common (49.3%). The proportion of EV tRNAs was highly variable, ranging between 0.04% to >95% depending on tissue source. EV tRNAs are present in a multitude of sources and show promise as disease markers in breast cancer, gastrointestinal cancers, and other diseases. EV tRNA research is an emerging field, with increasing numbers of papers highlighting novel methodologies for tRNA and tRNA fragment discovery.

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“…Yang et al firstly showed that exosomal tRF-1 was significantly up-regulated in serum from systemic lupus erythematosus (SLE) patients, which could serve as a non-invasive biomarker for the prediction and diagnosis of nephritis in SLE patients ( Yang et al, 2021 ). Furthermore, Meng et al reported the tRFs profile from atopic dermatitis (AD) patients, and suggested that plasma derived exosomal tRF-28 could serve as a potential biomarker for pediatric patients with AD ( Meng et al, 2021 ). In conclusion, different types of exosomal tRFs have been reported as potential diagnostic and predictive biomarkers for various diseases, however, so far, no study has investigated the potential biological functions of tRFs/tiRNAs in Exo-LT or Exo-AT.…”

Section: Discussionmentioning

confidence: 99%

Expression profiles of exosomal tRNA-derived fragments and their biological functions in lipomas

Zhou

Tao

Shao

et al. 2022

Front. Cell Dev. Biol.

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There is evidence that exosomes derived from the lipoma tissue (Exo-LT) have a stronger capacity to promote the proliferation and migration of adipose-derived stem cells (ADSCs) than those from the adipose tissue (Exo-AT). But the Exo-LT do not have a significant effect on the adipogenic differentiation of the ADSCs. Recently, certain exosomal tRNA-derived fragments (tRFs) have been shown to play a crucial role in the pathogenesis of certain tumors. Therefore, it is necessary to identify the differently expressed tRFs in Exo-LT to further elucidate their molecular functions in lipomas. High-throughput sequencing was performed to examine the tRFs and mRNAs from the all samples belonging to the Exo-LT and Exo-AT groups. Target prediction and bioinformatics analysis were performed to explore their downstream mRNAs and biological functions. In total, 456 differently expressed tRFs and tiRNAs were identified in the Exo-LT group, 12 of which were up-regulated and 12 were down-regulated, respectively. Notably, tRF-1001 was most obviously down-regulated and tRF-3004a was most obviously up-regulated in the Exo-LT group. Moreover, among the target genes of tRF-1001 and tRF-3004a, both JAG2 and VSIG4 were significantly down-regulated in the Exo-LT group, while WNT5A, COL1A1, and PPARGC1A were highly expressed in both the Exo-LT and Exo-AT groups. The significant down-regulation of JAG2 and VSIG4 in the Exo-LT group could be due to the fact that Exo-LT had a stronger capacity to promote the proliferation and migration of ADSCs compared to the Exo-AT. The high expression of WNT5A, COL1A1, and PPARGC1A in both the Exo-LT and Exo-AT groups could be due to the similar ability of Exo-LT and Exo-AT to promote the adipogenic differentiation of ADSCs.

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Transfer RNA‑derived fragment tRF‑28‑QSZ34KRQ590K in plasma exosomes may be a potential biomarker for atopic dermatitis in pediatric patients

Cited by 11 publications

References 23 publications

Differential 5′-tRNA Fragment Expression in Circulating Preeclampsia Syncytiotrophoblast Vesicles Drives Macrophage Inflammation

Differential 5′-tRNA Fragment Expression in Circulating Preeclampsia Syncytiotrophoblast Vesicles Drives Macrophage Inflammation

The Clinical Significance of Transfer RNAs Present in Extracellular Vesicles

Expression profiles of exosomal tRNA-derived fragments and their biological functions in lipomas

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