Expression profiles of exosomal tRNA-derived fragments and their biological functions in lipomas

Abstract

There is evidence that exosomes derived from the lipoma tissue (Exo-LT) have a stronger capacity to promote the proliferation and migration of adipose-derived stem cells (ADSCs) than those from the adipose tissue (Exo-AT). But the Exo-LT do not have a significant effect on the adipogenic differentiation of the ADSCs. Recently, certain exosomal tRNA-derived fragments (tRFs) have been shown to play a crucial role in the pathogenesis of certain tumors. Therefore, it is necessary to identify the differently expressed tRFs in Exo-LT to further elucidate their molecular functions in lipomas. High-throughput sequencing was performed to examine the tRFs and mRNAs from the all samples belonging to the Exo-LT and Exo-AT groups. Target prediction and bioinformatics analysis were performed to explore their downstream mRNAs and biological functions. In total, 456 differently expressed tRFs and tiRNAs were identified in the Exo-LT group, 12 of which were up-regulated and 12 were down-regulated, respectively. Notably, tRF-1001 was most obviously down-regulated and tRF-3004a was most obviously up-regulated in the Exo-LT group. Moreover, among the target genes of tRF-1001 and tRF-3004a, both JAG2 and VSIG4 were significantly down-regulated in the Exo-LT group, while WNT5A, COL1A1, and PPARGC1A were highly expressed in both the Exo-LT and Exo-AT groups. The significant down-regulation of JAG2 and VSIG4 in the Exo-LT group could be due to the fact that Exo-LT had a stronger capacity to promote the proliferation and migration of ADSCs compared to the Exo-AT. The high expression of WNT5A, COL1A1, and PPARGC1A in both the Exo-LT and Exo-AT groups could be due to the similar ability of Exo-LT and Exo-AT to promote the adipogenic differentiation of ADSCs.

Comprehensive analysis of lncRNA-miRNA-mRNA ceRNA network in exosomes from lipoma tissue reveals differential impacts on biological functions

“…Our study is the rst giving comparative analysis of Exo-LT and Exo-AT at the lncRNA, miRNA, and mRNA levels. In our previous study, we have analyzed difference in tRNA-derived fragments (tRFs) expression from Exo-LT and Exo-AT, and by identifying the differentially expressed tRFs in Exo-LT, we have preliminarily explained the reason for Exo-LT have a stronger capacity in promoting the proliferation and migration of ADSCs than Exo-AT (22,40). Now we have comprehensively analyzed the differences of lncRNAs, miRNAs and mRNAs expression in Exo-LT and Exo-AT, and constructed three ceRNA networks in Exo-LT, which we suggest that the unique expression of genes in Exo-LT affects its roles in promoting the proliferation and adipogenesis of ADSCs.…”

Expression and biological functions of tRNA-Derived Fragments Secreted by Extracellular Vesicles in sepsis-associated ARDS

“…Currently, with the application of high-throughput sequencing and the advances in bioinformatics techniques,increasing number of tRFs in extracellular vesicles have been reported to be associated with different disease [21] .It has been reported that 5′-tRFs wer present at different levels between preeclamptic and normotensive trophoblast tissues from the placenta [22] .Zhu et al demonstrated the presence of tRFs in exosomes of liver cancer cells [23] .Koi et al con rmed that the expression levels of tRF-Lys-TTT (tRF-32-PS5P4PW3FJHP1, 5′-tRNA half) were signi cantly elevated in breast cancer [24] .Zhang et al identi ed 11 upregulated tRFs and 18 downregulated tRFs in osteoporosis compared with healthy controls,and the expression levels of tRF-25-R9ODMJ6B26 (3′-tRF), tRF-38-QB1MK8YUBS68BFD2 (3′-tRF), and tRF-18-BS68BFD2 (3′-tRF) were signi cantly higher in osteoporosis [25] .…”