Transfer of resistance to potato leaf roll virus from Solanum brevidens into Solanum tuberosum by somatic fusion

Austin, S.; Baer, M.; Helgeson, John P.

doi:10.1016/0168-9452(85)90195-5

Cited by 185 publications

(49 citation statements)

References 19 publications

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“…Interspecific hybridization has been used to introgress useful traits from wild and related species into cultivated varieties in many Solanaceous crops, particularly in terms of pest and disease resistance (Austin et al 1985, 1988, Cubillos and Plaisted 1976, Hogenboom 1972, Maris 1989, Rick and Chetelat 1995, Simmonds 1966. Tomato, potato and tobacco have provided classic examples of investigations into genetic barriers such as unilateral incompatibility, post-fertilization embryo and/or endosperm abortion and hybrid weakness and sterility in interspecific gene exchange (Pickersgill 1997).…”

Section: Introductionmentioning

confidence: 99%

Overcoming Two Post-fertilization Genetic Barriers in Interspecific Hybridization between Capsicum annuum and C. baccatum for Introgression of Anthracnose Resistance

et al. 2006

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Interspecific hybridization is essential to introgress resistance genes from Capsicum baccatum, a related species of cultivated pepper (C. annuum), since reliable genetic resources resistant to anthracnose have recently been identified within the C. baccatum germplasm. In conventional interspecific hybridization between the two species, hybrids could not be generated due to embryo abortion, which has been known to be a postfertilization genetic barrier. Some partially compatible cross combinations, determined through observations of embryo development after pollination, were identified using a large number of accessions of C. annuum as pistillate parents. Embryo rescue technique was successfully employed to produce hybrids in these partially compatible crosses. Immature seeds bearing torpedo or early cotyledonary embryos, developed 35-40 days after pollination, were excised and the embryos were cultured on MS medium with sucrose and plant growth regulators. Hybridity was confirmed by observation of corolla yellow spot as a dominant speciesspecific trait of C. baccatum and using random amplified polymorphic DNA (RAPD) marker analysis. All the hybrid plants displayed vigorous growth but complete pollen sterility. The hybrid sterility was overcome through intensive backcrossing using C. annuum as the pollen parent. Consequently, hundreds of interspecific BC 1 F 1 progenies were raised, and introgression of anthracnose resistance was confirmed in this segregating population.

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Section: Introductionmentioning

confidence: 99%

Overcoming Two Post-fertilization Genetic Barriers in Interspecific Hybridization between Capsicum annuum and C. baccatum for Introgression of Anthracnose Resistance

et al. 2006

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“…For all these viruses, infection is systemic and resistance takes the form of a low virus titre in infected plants (Jones, 1979;Gibson et al, 1990). This resistance has been transferred to the S. tuberosum gene pool by somatic hybridization (Austin et al, 1985;Helgeson et al, 1986;Fish et aL, 1988;Gibson et al, 1988;Pehu et al, 1989Pehu et al, , 1990. Somatic hybrids (including aneuploids) between S. brevidens and PDH40, a dihaploid S. tuberosum genotype derived from cv.…”

Section: Introductionmentioning

confidence: 99%

Resistance in Solanum brevidens to both potato virus Y and potato virus X may be associated with slow cell-to-cell spread

Valkonen¹,

Pehu²,

Jones³

et al. 1991

Journal of General Virology

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A series of experiments was carried out to investigate the nature of the resistance of the wild potato species, Solanum brevidens, to potato virus X (PVX) and potato virus Y (PVY). In vitro inoculation of leaf protoplasts of S. brevidens and the virus-susceptible dihaploid S. tuberosum genotype PDH40 with PVX or PVY using polyethylene glycol showed that protoplasts of both species were similar in susceptibility. However, examination of protoplasts prepared from the leaves of S. tuberosum and S. brevidens inoculated 2 to 5 weeks earlier showed that the percentage of PVX-and PVYinfected leaf cells of S. tuberosum were, respectively, 45-to 100-fold and about 1000-fold greater than the percentage of infected leaf cells of S. brevidens. These results suggest that resistance in S. brevidens to both PVX and PVY could be associated with slow cell-tocell spread rather than with slow virus replication.

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“…(Helgeson et al 1998) and S. circaeifolium Bitter (Mattheij et al 1992), leaf roll virus from S. brevidens Phil. (Barsby et al 1984;Austin et al 1985; Helgeson et al 1986), insects from S. berthaultii Hawkes (Serraf et al 1991) and S. chacoense Bitter (Cheng et al 1995), frost damage from S. …”

mentioning

confidence: 99%

“…(Helgeson et al 1998) and S. circaeifolium Bitter (Mattheij et al 1992), leaf roll virus from S. brevidens Phil. (Barsby et al 1984;Austin et al 1985;Helgeson et al 1986), insects from S. berthaultii Hawkes (Serraf et al 1991) and S. chacoense Bitter , frost damage from S. acaule Bitter (Rokka et al 1998) and S. commersonii Dun. (Cardi et al 1993), and herbicide tolerance from S. nigrum L. (Binding et al 1982).…”

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confidence: 99%

Somatic hybridization between Solanum tuberosum and S. cardiophyllum

Shi¹,

Chen²,

Li³

et al. 2006

Can. J. Plant Sci.

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. 2006. Somatic hybridization between Solanum tuberosum and S. cardiophyllum. Can. J. Plant Sci. 86: 539-545. The wild diploid Mexican species, Solanum cardiophyllum Lindl. (2n = 2x = 24), is resistant to important potato diseases. However, introgression of resistance to the tetraploid cultivated potato (S. tuberosum L.) (2n = 4x = 48) by conventional crossing is not feasible due to the difference in their endosperm balance number between these species. Somatic hybrids between S. cardiophyllum and S. tuberosum were produced for the first time by electrofusion of protoplasts isolated from young leaves of each parental line. The hybrid nature of the regenerated plants was confirmed based on morphology, chromosome number and DNA species-specific RAPD markers. All the somatic hybrids produced a violet pigmentation on their stems and petioles, which resembled the wild partner. Most of the hybrid plants had 2n = 72 chromosomes and exhibited a morphology intermediate between the two fusion parents, but with a tendency towards cultivated potato. These plants flowered and set fruit when backcrossed with their S. tuberosum fusion parent. Mots clés: Pomme de terre (Solanum tuberosum), S. cardiophyllum, fusion des protoplastes, marqueur RAPD Potatoes (Solanum tuberosum L.) are plagued by many kinds of diseases. Of these diseases, late blight, caused by Phytophthora infestans (Mont.) de Bary, is the most devastating one worldwide. Under cool wet conditions, the pathogen rapidly attacks the leaves, stems and tubers, causing significant reductions in yield and quality. All of the current commercial potato cultivars are highly susceptible to late blight. Surveys have shown that the disease costs potato growers $3 billion annually worldwide . Control of potato late blight is currently achieved through the extensive use of fungicides. Therefore, it is not surprising that seeking a genetic resource with resistant traits to the disease has long attracted potato breeders' attention. For example, the Mexican wild species Solanum demissum Lindl. was reported to be resistant to late blight (Reddick 1930). Unfortunately, the resistance was only effective against certain races of the pathogen (Black and Gallegly 1957). An accession of another Mexican wild species, S. cardiophyllum Lindl., was recently found to possess a high level of resistance to late blight . However, S. cardiophyllum and the cultivated potato, S. tuberosum are reproductively incompatible due to differences in their endosperm balance numbers although they can be crossed with several other diploid Mexican wild species (Chen et al. 2004). Thus, this prevents the exploitation of this valuable germplasm using conventional breeding methods.Protoplast fusion offers an alternative to conventional crossing as it provides a means of circumventing the sexual reproduction barriers and allows the incorporation of desirable traits from wild species into cultivated potatoes. Using somatic hybridization, several resistance genes have been introduced into cultivated potato from wild s...

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Transfer of resistance to potato leaf roll virus from Solanum brevidens into Solanum tuberosum by somatic fusion

Cited by 185 publications

References 19 publications

Overcoming Two Post-fertilization Genetic Barriers in Interspecific Hybridization between Capsicum annuum and C. baccatum for Introgression of Anthracnose Resistance

Overcoming Two Post-fertilization Genetic Barriers in Interspecific Hybridization between Capsicum annuum and C. baccatum for Introgression of Anthracnose Resistance

Resistance in Solanum brevidens to both potato virus Y and potato virus X may be associated with slow cell-to-cell spread

Somatic hybridization between Solanum tuberosum and S. cardiophyllum

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