Transfer of IVF-contaminated blastocysts with removal of the zona pellucida resulted in live births

Shu, Yaqing; Prokai, David; Berga, Sarah L.; Taylor, Robert B.; Johnston-MacAnanny, Erika B.

doi:10.1007/s10815-016-0776-3

Cited by 14 publications

(14 citation statements)

References 13 publications

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“…The viability of IVF embryos, in turn, depends on the composition of embryo culture media and physical environmental factors applied in embryo culture. At the same time bacterial contamination of gamete samples used in ART may impair the embryo culture environment, causing damage to or even loss of cultured oocytes and embryos [1].…”

Section: Introductionmentioning

confidence: 99%

The complex microbiome from native semen to embryo culture environment in human in vitro fertilization procedure

Štšepetova

Baranova

Simm

et al. 2020

Reprod Biol Endocrinol

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Background: Only a few microbial studies have conducted in IVF (in vitro fertilization), showing the high-variety bacterial contamination of IVF culture media to cause damage to or even loss of cultured oocytes and embryos. We aimed to determine the prevalence and counts of bacteria in IVF samples, and to associate them with clinical outcome. Methods: The studied samples from 50 infertile couples included: raw (n = 48), processed (n = 49) and incubated (n = 50) sperm samples, and IVF culture media (n = 50). The full microbiome was analyzed by 454 pyrosequencing and quantitative analysis by real-time quantitative PCR. Descriptive statistics, t-, Mann-Whitney tests and Spearman's correlation were used for comparison of studied groups. Results: The study involved normozoospermic men. Normal vaginal microbiota was present in 72.0% of female partners, while intermediate microbiota and bacterial vaginosis were diagnosed in 12.0 and 16.0%, respectively. The decreasing bacterial loads were found in raw (35.5%), processed (12.0%) and sperm samples used for oocyte insemination (4.0%), and in 8.0% of IVF culture media. The most abundant genera of bacteria in native semen and IVF culture media were Lactobacillus, while in other samples Alphaproteobacteria prevailed. Staphylococcus sp. was found only in semen from patients with inflammation. Phylum Bacteroidetes was in negative correlation with sperm motility and Alphaproteobacteria with high-quality IVF embryos. Conclusion: Our study demonstrates that IVF does not occur in a sterile environment. The prevalent bacteria include classes Bacilli in raw semen and IVF culture media, Clostridia in processed and Bacteroidia in sperm samples used for insemination. The presence of Staphylococcus sp. and Alphaproteobacteria associated with clinical outcomes, like sperm and embryo quality.

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Section: Introductionmentioning

confidence: 99%

The complex microbiome from native semen to embryo culture environment in human in vitro fertilization procedure

Štšepetova

Baranova

Simm

et al. 2020

Reprod Biol Endocrinol

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“…This study showed that when the environmental contamination of the IVF culture room leads to contamination of the embryo culture medium, it is feasible to take measures such as strengthening the daily rapid flushing of the culture medium and reducing blastocyst culture as remedial treatment. In addition, there are other methods, such as the removal of the zona pellucida of contaminated frozen blastocysts [24]. However, the present study has some limitations.…”

Section: Discussionmentioning

confidence: 86%

Analysis of the clinical outcomes of microbial contamination caused by environmental contamination of the embryology laboratory during IVF-ET treatment cycles

Zheng

Chen

et al. 2023

BMC Pregnancy Childbirth

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Background Bacterial contamination may cause loss of or damage to cultured oocytes or embryos, resulting in the lack of transplantable embryos during IVF embryo culture. However, there are few reports about IVF embryo contamination caused by embryology laboratories. In this work, we evaluated clinical pregnancy outcomes and the risk of maternal and infant complications after embryo contamination caused by environmental pollution during IVF. Methods The authors retrospectively analyzed 2490 IVF-ET ovulation induction therapy cycles in the Reproductive Center of Yichang Central People's Hospital from January 2015 to May 2022. According to the presence or absence of embryo culture medium contamination, the two groups were divided into an embryo contamination cycle and a nonembryo contamination cycle. The primary outcome parameters were the characteristics and progress of embryo culture medium contamination. Embryo laboratory outcomes, pregnancy outcomes, and maternal and infant complications were secondary outcome parameters. Results One case of embryo contamination originated from semen contamination. The remaining 15 cases involved environmental contamination outbreaks in embryo culture chambers, caused by Staphylococcus pasteuri. Compared with conventional uncontaminated IVF cycles, the 15 cases of contaminated embryo cycles showed no significant difference in embryo laboratory outcomes, pregnancy outcomes, or maternal and infant complications except for a slightly higher rate of fetal growth retardation. Ultimately, 11 live-born infants were successfully delivered, of which 2 were premature. The remaining 4 patients did not become pregnant after 1–2 transfers due to a lack of transferable embryos. Conclusion When the embryo culture medium is contaminated due to the environmental contamination of the IVF culture room, it is feasible to perform daily rapid rinsing of the culture medium and avoid blastocyst culture as remedial treatment. However, the long-term impact on offspring needs further prospective research.

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“…In 1 other report, Shu et al. ( 10 ) removed the ZP of contaminated frozen blastocysts, which led to successful clinical pregnancies after the transfer of ZP-free blastocysts that were previously contaminated during IVF culture. However, the removal of the ZP in that study was not done on the day the contamination was discovered but right before embryo transfer.…”

Section: Discussionmentioning

confidence: 99%

A new method to rescue embryos contaminated by bacteria

et al. 2022

F&S Reports

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Transfer of IVF-contaminated blastocysts with removal of the zona pellucida resulted in live births

Cited by 14 publications

References 13 publications

The complex microbiome from native semen to embryo culture environment in human in vitro fertilization procedure

The complex microbiome from native semen to embryo culture environment in human in vitro fertilization procedure

Analysis of the clinical outcomes of microbial contamination caused by environmental contamination of the embryology laboratory during IVF-ET treatment cycles

A new method to rescue embryos contaminated by bacteria

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