A new method to rescue embryos contaminated by bacteria

Li, Ruiqi; Du, Fengjiao; Ou, Songbang; Ouyang, Nengyong; Wang, Wenjun

doi:10.1016/j.xfre.2022.05.002

Cited by 8 publications

(9 citation statements)

References 13 publications

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“…However, it may not be the most effective approach, as demonstrated by the patient in our study who experienced failed pregnancies despite careful washing of the embryos during the second and third transfers. This could be attributed to the high bacterial load in the culture medium and the qualitative nature of the washing procedure, leading to recontamination 4 …”

Section: Discussionmentioning

confidence: 99%

“…This could be attributed to the high bacterial load in the culture medium and the qualitative nature of the washing procedure, leading to recontamination. 4 The zona pellucida is a critical structure that surrounds the oocyte and later, the developing embryo in humans. The ultrastructure of zona pellucida has been clearly described in a related study.…”

Section: Discussionmentioning

confidence: 99%

“…However, a study has demonstrated that washing alone is not effective in eliminating bacteria, as some still remain in the zona pellucida 3 . In a recent case report by Li R et al., 4 a new method was proposed to rescue contaminated embryos, involving the removal of the contaminated zona pellucida using Tyrode acid. It has been observed that the porous structure of the zona pellucida makes it difficult to completely eliminate microorganisms 5 .…”

Section: Introductionmentioning

confidence: 99%

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Zona pellucida removal resulted in a successful live birth: Report on a case with recurrent implantation failure due to embryonic bacteria contamination

Dang,

Tran,

et al. 2024

Clinical Case Reports

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Key clinical messageIn in vitro fertilization (IVF), laser offers several advantages. In this study, we employed laser to eliminate the zona pellucida of a contaminated embryo. This approach helps to rescue embryo with bacterial contamination, and improve embryo‐endometrium interaction.AbstractTo present a case report on the removal of a contaminated zona pellucida from an embryo of patient with a history of recurrent implantation failure (RIF), which was followed by a successful live birth. We present the case of a 34‐year‐old patient with a history of 3 years of infertility who underwent in vitro fertilization. During the culture process, the embryos became contaminated, leading to three failed implantations. Despite the aneuploidy of the embryo and the implementation of a washing technique, the contamination persisted. In the final attempt, the contaminated zona pellucida was successfully removed using laser, followed by embryo transfer, resulting in a live birth. We provided detailed clinical information, including patient demographics, infertility history, ovarian response, evidence of bacterial contamination, embryo development, treatment protocols, and outcomes. Laser excision of the zona pellucida is a safe and effective method for addressing bacterial infection in embryos.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Zona pellucida removal resulted in a successful live birth: Report on a case with recurrent implantation failure due to embryonic bacteria contamination

Dang,

Tran,

et al. 2024

Clinical Case Reports

View full text Add to dashboard Cite

show abstract

“…It has the potential to replace traditional clean energy strategies based on solar energy conversion [1,2]. The combination of incredible benefits, such as superb tolerance for perovskite structure defects [3], as well as the availability of outstanding efficient carriers mobility, light (photon) absorption efficiency, and sufficient carrier diffusion lifetimes, made this a strong contender for exceptional solar cell photovoltaic applications and performance [4,5]. Since 2009, when the organic metal halide perovskite was first incorporated into the titanium dioxide (TiO 2 ) structure with a moderate efficiency of 3.8 percent, perovskite materials device efficiency has 24.2 percent or surpassed this percentage, with an overall average of 24 percent certified by the Fraunhofer Institute for solar energy system [6].…”

Section: Introductionmentioning

confidence: 99%

Lithium-based perovskites materials for photovoltaic solar cell and protective rays window applications: a first-principle calculations

et al. 2023

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Perovskites are the key enabler materials for the solar cell applications in the achievement of high performance and low production costs. In this article, the structural, mechanical, electronic, and optical properties of rubidium-based cubic nature perovskite LiHfO3 and LiZnO3 are investigated. These properties are investigated using density-functional theory with the aid of CASTEP software by introducing ultrasoft pseudo-potential plane-wave (USPPPW) and GG-approximation-PB-Ernzerhof exchange–correlation functionals. It is investigated that the proposed compounds exhibit stable cubic phase and meet the criteria of mechanical stability by the estimated elastic properties. Also, according to Pugh's criterion, it is noted that LiHfO3 is ductile and LiZnO3 is brittle. Furthermore, the electronic band structure investigation of LiHfO3 and LiZnO3 shows that they have indirect bandgap (BG). Moreover, the BG analysis of the proposed materials shows that these are easily accessible. Also, the results for partial density of states (DOS) and total DOS confirm the degree of a localized electron in the distinct band. In addition, the optical transitions in the compounds are examined by fitting the damping ratio for the notional dielectric functions scaling to the appropriate peaks. At absolute zero temperature, the materials are observed as semiconductors. Therefore, it is evident from the analysis that the proposed compounds are excellent candidates for solar cells and protective rays applications.

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“…To solve the problem of composite insulator defect detection in operation, various detection methods have been studied at home and abroad [8][9][10][11]. In [12], the phase resolved partial discharge (PRPD) spectrum is used to determine the operating state of an insulator using the pulse current method. In [13], the 3D electric field of an insulator was simulated using finite element software, and the effect of surrounding air conductivity on the electric field was evaluated.…”

Section: Introductionmentioning

confidence: 99%

Composite Insulator Defect Identification Method Based on Acoustic–Electric Feature Fusion and MMSAE Network

et al. 2023

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Aiming to solve the partial discharge problem caused by defects in composite insulators, most existing live detection methods are limited by the subjectivity of human judgment, the difficulty of effective quantification, and the use of a single detection method. Therefore, a composite insulator defect diagnosis model based on acoustic–electric feature fusion and a multi-scale perception multi-input of stacked auto-encoder (MMSAE) network is proposed in this paper. Initially, during the withstanding voltage experiment, the electromagnetic wave spectrometer and ultrasonic detector were used to collect and process the data of six types of composite insulator samples with artificial defects. The electromagnetic wave spectrum, ultrasonic power spectral density, and n-S map were then obtained. Then, the network architecture of MMSAE was built by integrating a stacked auto-encoder and multi-scale perception module; the feature extraction and fusion methods of the electromagnetic wave spectrum and ultrasonic signal were investigated. The proposed method was used to diagnose test samples, and the diagnostic results were compared to those obtained using a single input source and the artificial neural network (ANN) method. The results demonstrate that the detection accuracy of acoustic–electric feature fusion is greater than that of a single feature; the accuracy of the proposed method is 99.17%, which is significantly higher than the accuracy of the conventional ANN method. Finally, composite insulator defect diagnosis software based on PYQT5 and Keras was developed. Ten 500 kV aging composite insulators were used to validate the effectiveness of the proposed method and design software.

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A new method to rescue embryos contaminated by bacteria

Cited by 8 publications

References 13 publications

Zona pellucida removal resulted in a successful live birth: Report on a case with recurrent implantation failure due to embryonic bacteria contamination

Zona pellucida removal resulted in a successful live birth: Report on a case with recurrent implantation failure due to embryonic bacteria contamination

Lithium-based perovskites materials for photovoltaic solar cell and protective rays window applications: a first-principle calculations

Composite Insulator Defect Identification Method Based on Acoustic–Electric Feature Fusion and MMSAE Network

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