Transfer of condensed viral DNA into eukaryotic cells using proteoliposomes

Tikchonenko, T.I.; Glushakova, Svetlana; Kislina, O S; Grodnitskaya, N. A.; Маныкин, А. А.; Naroditsky, Boris S.

doi:10.1016/0378-1119(88)90535-5

Cited by 25 publications

(14 citation statements)

References 26 publications

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“…Encapsulation of spermine pre-condensed linear DNA into liposomes was previously reported. 49 However, the DNA concentration was 10-to 100-fold lower than we have used. Maximal loading efficiency of these liposomes appeared to be in the range of 0.02-0.2 g/ mol DNA/lipid.…”

Section: Figure 11 Fluorescent Photomicrographs Of Ovcar-3 Cells Tranmentioning

confidence: 74%

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A novel N-acyl phosphatidylethanolamine-containing delivery vehicle for spermine-condensed plasmid DNA

Shangguan¹,

Cabral-Lilly²,

Purandare³

et al. 2000

Gene Ther

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A unique method for formulation of plasmid DNA with phospholipids has been devised for the purpose of producing vehicles that can mediate gene delivery and transfection of living cells. The polycation, spermine, was used to condense plasmid DNA within a water-in-chloroform emulsion stabilized by phospholipids. After organic solvent removal, the particles formed could be extruded to a number average size of about 200 nm and retained DNA that was protected from nuclease digestion. This resulted in a relatively high protected DNA-to-lipid ratio of approximately 1 g DNA/ mol lipid. The size distribution of the preparation was relatively homogeneous as judged by light microscopy and quasi-elastic light scattering. Electron microscopic studies showed structural heterogeneity, but suggested that at least some of the plasmid DNA in this preparation was in the form of the previously observed spermine-condensed bent rods and toroids and was encapsulated within liposomal membranes.

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Section: Figure 11 Fluorescent Photomicrographs Of Ovcar-3 Cells Tranmentioning

confidence: 74%

“…Since condensation can reduce the size of plasmid DNA, there have been attempts to encapsulate DNA condensed with polylysine 6 or polyamines [47][48][49] into liposomes. Encapsulation of spermine pre-condensed linear DNA into liposomes was previously reported.…”

Section: Figure 11 Fluorescent Photomicrographs Of Ovcar-3 Cells Tranmentioning

confidence: 99%

A novel N-acyl phosphatidylethanolamine-containing delivery vehicle for spermine-condensed plasmid DNA

Shangguan¹,

Cabral-Lilly²,

Purandare³

et al. 2000

Gene Ther

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“…In keeping with this idea, we were able to obtain the polyamine putrescine by thermal decarboxylation of the amino acid ornithine under possible prebiotic conditions (Wong et al, 1991); then, it might have been possible that in precellular conditions polyamines caused nucleic acid condensation before that of protein formation. In addition, nucleic acid-polyamine complexes have some important nucleic acid protective properties, and DNA-spermidine-complexes are transcriptionally more active than DNA without polyamine condensation (Bocian et al, 1978;Johnson and Bach, 1966;Tikchonenko et al, 1988;Tabor and Tabor, 1984;Baeza et al, 1987b). Furthermore, an increase in the catalytic properties of RNA was shown in RNA-spermidine complexes (Guerrier et al, 1983).…”

Section: Introductionmentioning

confidence: 94%

“…DNA condensea by potyamines and proteins has been used for efficient incorporation of long linear or circular forms of DNA into vesicles (Jay and Gilbert, 1987;Tikchonenko et aL, 1988;Szelei and Duda, 1989) because compacted forms of DNA are more appropriate in size to fit into liposomes. In this study we compared the efficiency of incorporation of compacted forms of (3H)SV40 DNA obtained with spermidine and Co3+(NH3)6 into REP liposomes from egg-yolk phosphatidylcholine.…”

Section: Encapsulation Of Condensed Forms Of Pbr322 Dnamentioning

confidence: 99%

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Possible prebiotic significance of polyamines in the condensation, protection, encapsulation, and biological properties of DNA

Baeza¹,

Ibáñez²,

Wong³

et al. 1992

Origins Life Evol Biosphere

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Some properties of DNA condensed with spermidine have been compared with the properties of DNA condensed with Co3+(NH3)6 to determine whether condensation of DNA with these trivalent cations protects DNA against the action of DNase I and increases transcription and encapsulation of DNA into liposomes. It was shown that DNA condensed with Co3+(NH3)6 was resistant to the action of the endonuclease DNase I such as DNA condensed with spermidine was. However, DNA condensed with Co3+(NH3)6 was significantly less active in transcription with the E. coli RNA polymerase than DNA-spermidine condensed forms. In addition, it was demonstrated that both compacted forms of DNA were more efficiently encapsulated into neutral liposomes; however, negatively, charged liposomes were scarcely formed in the presence of DNA condensed with Co3+(NH3)6. These experiments and the well documented properties of polyamines increasing the resistance to radiations and hydrolysis of nucleic acids, as well as their biological activities, such as replication, transcription, and translation, together with the low concentration of Co3+ in the environment, lead us to propose spermidine as a plausible prebiotic DNA condensing agent rather than Co3+ and the basic proteins proposed by other authors. Then, we consider the possible role and relevance of the polyamine-nucleic acids complexes in the evolution of life.

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Nonviral Methods for Gene Transfer

Djeha

Lashford

1999

Blood Cell Biochemistry

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Transfer of condensed viral DNA into eukaryotic cells using proteoliposomes

Cited by 25 publications

References 26 publications

A novel N-acyl phosphatidylethanolamine-containing delivery vehicle for spermine-condensed plasmid DNA

A novel N-acyl phosphatidylethanolamine-containing delivery vehicle for spermine-condensed plasmid DNA

Possible prebiotic significance of polyamines in the condensation, protection, encapsulation, and biological properties of DNA

Nonviral Methods for Gene Transfer

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