Transfection of Neuroprogenitor Cells with Iron Nanoparticles for Magnetic Resonance Imaging Tracking: Cell Viability, Differentiation, and Intracellular Localization

Miyoshi, Sosuke; Flexman, Jennifer A.; Cross, Donna; Maravilla, Kenneth R.; Kim, Yongmin; Anzai, Yoshimi; Oshima, Junko; Minoshima, Shinsei

doi:10.1007/s11307-005-0008-1

Cited by 54 publications

(41 citation statements)

References 18 publications

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“…22 MIRB labeling can occur without use of transfection reagents that might induce cell damage. 11,23,24 Once labeled, MIRB resides in LAMP1+ lysosomes.…”

Section: Resultsmentioning

confidence: 99%

Human neural progenitor cells retain viability, phenotype, proliferation, and lineage differentiation when labeled with a novel iron oxide nanoparticle, Molday ION Rhodamine B

Shen¹,

Plachez²,

Chan³

et al. 2013

IJN

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Ultrasmall superparamagnetic iron-oxide particles (USPIOs) loaded into stem cells have been suggested as a way to track stem cell transplantation with magnetic resonance imaging, but the labeling, and post-labeling proliferation, viability, differentiation, and retention of USPIOs within the stem cells have yet to be determined for each type of stem cell and for each type of USPIO. Molday ION Rhodamine B™ (BioPAL, Worcester, MA, USA) (MIRB) has been shown to be a USPIO labeling agent for mesenchymal stem cells, glial progenitor cells, and stem cell lines. In this study, we have evaluated MIRB labeling in human neuroprogenitor cells and found that human neuroprogenitor cells are effectively labeled with MIRB without use of transfection reagents. Viability, proliferation, and differentiation properties are unchanged between MIRB-labeled neuroprogenitors cells and unlabeled cells. Moreover, MIRB-labeled human neuroprogenitor cells can be frozen, thawed, and replated without loss of MIRB or even without loss of their intrinsic biology. Overall, those results show that MIRB has advantageous properties that can be used for cell-based therapy.

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“…22 MIRB labeling can occur without use of transfection reagents that might induce cell damage. 11,23,24 Once labeled, MIRB resides in LAMP1+ lysosomes.…”

Section: Resultsmentioning

confidence: 99%

Human neural progenitor cells retain viability, phenotype, proliferation, and lineage differentiation when labeled with a novel iron oxide nanoparticle, Molday ION Rhodamine B

Shen¹,

Plachez²,

Chan³

et al. 2013

IJN

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show abstract

“…116 Second, coupling of transfection agents like Superfect ® , poly-L-lysine, protamine, and Lipofectamine ® with SPIONs can strongly increase the efficiency of stem cell labeling. [117][118][119] Third, a magnetic field can be used to increase membrane permeability transiently, resulting in accumulation of SPIONs within the cells. 120 However, stem cells can also be labeled by conjugating SPIONs to their cellular surface.…”

Section: Stem Cell Therapymentioning

confidence: 99%

Superparamagnetic iron oxide nanoparticles: magnetic nanoplatforms as drug carriers

Wahajuddin¹,

Arora²

2012

IJN

884

574

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“…MR of cells labeled with an MR contrast agent may be the most promising means for achieving the goal. MR of cells labeled with SPIO has been shown to be a sensitive method of noninvasively tracking various cell populations in the brain, [12][13][14][15][16][17] bone marrow, 18-20 kidneys, 21,22 and myocardial tissue. [23][24][25] However, relatively few in vivo studies have assessed quantifying the recruitment of EPCs by intravenous injection after liver injury using a clinical 3.0T MR system.…”

Section: Discussionmentioning

confidence: 99%

R2* and R2 mapping for quantifying recruitment of superparamagnetic iron oxide-tagged endothelial progenitor cells to injured liver: tracking in vitro and in vivo

Wang¹,

Li²,

Quan³

et al. 2014

IJN

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Objective To evaluate clinical 3.0T magnetic resonance for tracking and quantifying superparamagnetic iron oxide (SPIO)–labeled endothelial progenitor cells (EPCs) in vitro and homing to liver with acute injury in vivo. Methods The bone marrow-derived EPCs were isolated and cultured for 4 days and examined in vitro for lineage markers. Then the cultured cells were labeled with a ferumoxides-protamine sulfate complex. Iron uptake was analyzed with an electron microscope and Prussian blue staining. Agarose gel phantoms containing different amounts of EPCs (0–2.5 × 10 6 cells per milliliter of 1.0% agarose gel) were analyzed with 3.0T R2 and R2* relaxometry. For in vivo tracking, liver injury was induced in healthy C57 mice (female, 6 weeks old, weight 19–20 g) by administration of carbon tetrachloride by single intraperitoneal injection. The R2* and R2 mapping of injured and normal livers of C57 mice were conducted by using 3.0T magnetic resonance on Days 0, 1, 4, and 8 after intravenous SPIO-tagged cells transplantation. Results Electron microscope and Perls Prussian blue stain revealed the efficiency of SPIO particles uptake was more than 95% and no structural changes of labeled cells were found compared with control group. R2 and R2* values were linearly correlated with the number of iron-loaded cells in the agarose gel phantoms, and R2* values were significantly greater than R2 ( P <0.01). R2* values in all groups were obviously greater than R2 ( P <0.01). The R2* values of the injured livers were greater than normal on Days 1 and 4 ( P <0.01). No significant difference of R2 values could be found among the three groups. Conclusion Quantitative R2* mapping provides a useful method for quantifying intravascular administered SPIO-tagged EPCs homing to injured livers.

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Transfection of Neuroprogenitor Cells with Iron Nanoparticles for Magnetic Resonance Imaging Tracking: Cell Viability, Differentiation, and Intracellular Localization

Abstract: These findings indicate that HVJ-Es are an effective vehicle for SPIO transfection of NPCs. The intracellular localization after differentiation raises the question as to the capability of MRI to distinguish cell migration from axonal or dendritic growth in vivo.

Cited by 54 publications

References 18 publications

Human neural progenitor cells retain viability, phenotype, proliferation, and lineage differentiation when labeled with a novel iron oxide nanoparticle, Molday ION Rhodamine B

Human neural progenitor cells retain viability, phenotype, proliferation, and lineage differentiation when labeled with a novel iron oxide nanoparticle, Molday ION Rhodamine B

Superparamagnetic iron oxide nanoparticles: magnetic nanoplatforms as drug carriers

R2* and R2 mapping for quantifying recruitment of superparamagnetic iron oxide-tagged endothelial progenitor cells to injured liver: tracking in vitro and in vivo

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