Transfection and intracellular trafficking properties of carbon dot-gold nanoparticle molecular assembly conjugated with PEI-pDNA

Kim, Jinhwan; Park, Juhee; Kim, Hyun Woo; Singha, Kaushik; Kim, Won Jong

doi:10.1016/j.biomaterials.2013.05.072

Cited by 150 publications

(92 citation statements)

References 48 publications

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“…As shown in Fig. 1e, when applied on 293t cells, DMP micelle were much less toxic with its IC 50 exceeded the concentration of 1.2 mg mL À1 , while that of PEI25K (''gold standard'' transfection agent 33,34 ) was estimated to be smaller than 0.1 mg mL À1 . This indicated that DMP micelles were much safer that conventional used PEI25K transfection agent which showed a tremendous toxicity.…”

Section: Resultsmentioning

confidence: 98%

Delivery of interleukin-22 binding protein (IL-22BP) gene by cationic micelle for colon cancer gene therapy

et al. 2018

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Section: Resultsmentioning

confidence: 98%

Delivery of interleukin-22 binding protein (IL-22BP) gene by cationic micelle for colon cancer gene therapy

et al. 2018

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“…111 Other delivery systems based upon gold nanoparticles include gold nanoparticles passivated with BSA-SV40 large T antigen conjugates, 114 layer-by-layer gold nanoparticles with polymers for the delivery of siRNA 115, 116 and plasmid DNA delivery using carbon dot-gold nanoparticles conjugated with PEI. 117 These exciting techniques and technologies all may have the potential to be applied to mRNA delivery, demonstrating that the field of gold nanoparticle-mediated mRNA transport is rich with new possibilities.…”

Section: Gold Nanoparticlesmentioning

confidence: 99%

Biomaterials for mRNA delivery

et al. 2015

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Messenger RNA (mRNA) has recently emerged with remarkable potential as an effective alternative to DNA-based therapies because of several unique advantages. mRNA does not require nuclear entry for transfection activity and has a negligible chance of integrating into the host genome which excludes the possibility of potentially detrimental genomic alternations. Chemical modification of mRNA has further enhanced its stability and decreased its activation of innate immune responses. Additionally, mRNA has been found to have rapid expression and predictable kinetics. Nevertheless, the ubiquitous application of mRNA remains challenging given its unfavorable attributes, such as large size, negative charge and susceptibility to enzymatic degradation. Further refinement of mRNA delivery modalities is therefore essential for its development as a therapeutic tool. This review provides an exclusive overview of current state-of-the-art biomaterials and nanotechnology platforms for mRNA delivery, and discusses future prospects to bring these exciting technologies into clinical practice.

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“…17 Nevertheless, little research on CD-based gene delivery has been reported thus far. 19,20 We believe the combination of fluorescent CDs with gene delivery function is promising for real-time tracking of gene transfection and shedding light on transfection mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Polycation-b-Polyzwitterion Copolymer Grafted Luminescent Carbon Dots As a Multifunctional Platform for Serum-Resistant Gene Delivery and Bioimaging

Liu

Zhai

et al. 2014

ACS Appl. Mater. Interfaces

114

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Nanomaterials that integrate functions of imaging and gene delivery have been of great interest due to their potential use in simultaneous diagnosis and therapy. Herein, polycation-b-polysulfobetaine block copolymer, poly[2-(dimethylamino) ethyl methacrylate]-b-poly[N-(3-(methacryloylamino) propyl)-N,N-dimethyl-N-(3-sulfopropyl) ammonium hydroxide] (PDMAEMA-b-PMPDSAH) grafted luminescent carbon dots (CDs) were prepared via surface-initiated atom transfer radical polymerization (ATRP) and investigated as a multifunctional gene delivery system (denoted as CD-PDMA-PMPD) in which the CD cores acted as good multicolor cell imaging probes, the cationic PDMAEMA acted as a DNA condensing agent, and the outer shell of zwitterionic PMPDSAH block protected the vector against nonspecific interactions with serum components. As revealed by the fluorescent spectrum study, the photoluminescent attributes, especially the tunable emission property, were well inherited from the parent CDs. The CD-PDMA-PMPD could condense plasmid DNA into nanospheres with sizes of approximate 50 nm at a proper complex ratio, posing little cytotoxicity at higher ratios. It was shown that the hybrid vector exhibited significantly suppressed BSA protein adsorption and superior hemocompatibility compared to those of the widely used PEI25k. In the in vitro transfection assay, an increased serum concentration from 10 to 50% caused a dramatic drop in PEI25k transfection performance, whereas the transfection efficiency of CD-PDMA-PMPD was well maintained; CD-PDMA80-PMPD40 showed 13 and 28 times higher transfection efficiencies than PEI25k at 30 and 50% serum concentration, respectively. Intriguingly, the carbon dots in the transfected cells displayed excitation-dependent fluorescent emissions, portending that this polycation-polyzwitterion modified CD will be a promising theranostic vector with excellent stealth performance.

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Transfection and intracellular trafficking properties of carbon dot-gold nanoparticle molecular assembly conjugated with PEI-pDNA

Cited by 150 publications

References 48 publications

Delivery of interleukin-22 binding protein (IL-22BP) gene by cationic micelle for colon cancer gene therapy

Delivery of interleukin-22 binding protein (IL-22BP) gene by cationic micelle for colon cancer gene therapy

Biomaterials for mRNA delivery

Polycation-b-Polyzwitterion Copolymer Grafted Luminescent Carbon Dots As a Multifunctional Platform for Serum-Resistant Gene Delivery and Bioimaging

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