Transduction of Auxotrophic Markers in a Chloramphenicol-producing Strain of Streptomyces

Stuttard, C.

doi:10.1099/00221287-110-2-479

Cited by 50 publications

(33 citation statements)

References 15 publications

(10 reference statements)

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“…As the 1970s came to an end, generalized transduction was added to the other two modes of gene transfer -by plasmid-mediated conjugation and by transformation (albeit artificial) -when Colin Stuttard described a phage, SV1, that could transduce markers in Streptomyces venezuelae (Stuttard, 1979). Unfortunately, SV1 did not work in any other species ; and the second example of general transduction, by SF1 in Streptomyces fradiae (Chung, 1982), was also taxonomically circumscribed.…”

Section: Figmentioning

confidence: 99%

“…An intriguing feature of the chromosome first observed in the mid-1960s, but still awaiting evaluation, is the indication of an ancestral Braendle & Szybalski (1957), Hopwood (1957), Saito (1957Saito ( ) 1958 Rudimentary linkage analysis Hopwood (1958Hopwood ( , 1959Hopwood ( ) 1960 Heteroclones discovered Sermonti et al (1960), Hopwood et al (1963Hopwood et al ( ) 1965 Single (circular) linkage group Hopwood (1965bHopwood ( ) 1967 Ancestral genome duplication ? Hopwood (1967a) Discovery of A-factor (in S. griseus) Khokhlov et al (1967) 1969-73 The SCP1 plasmid and fertility Hopwood et al (1969), Vivian & Hopwood (1971, 1973, Vivian (1971), Hopwood & Wright (1973b Discovery of φC31 and S. lividans 66 Lomovskaya et al (1970) 1970-72 Morphological mutants mapped Hopwood et al (1970), Chater (1972Chater ( ) 1974 Protoplast formation and regeneration Okanishi et al (1974) 1975 First CCC plasmid (SCP2) isolated Schrempf et al (1975) Methylenomycin genes are on SCP1 Kirby et al (1975), Kirby & Hopwood (1977) 1976 Antibiotic-pathway mutants mapped to the chromosome Wright & Hopwood (1976b) 1977 Recombination via protoplast fusion Hopwood et al (1977), Baltz (1978) 1978 Protoplast transformation Bibb et al (1978) Plasmid-induced ' pocks ' Bibb et al (1978' Bibb et al ( ) 1979 Transduction discovered (in S. venezuelae) Stuttard (1979) Linear plasmids discovered (in S. rochei) Hayakawa et al (1979) 1981 Integrating plasmids discovered Bibb et al (1981) duplication of the genome. I noticed a t...…”

Section: The In Vivo Yearsmentioning

confidence: 99%

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Forty years of genetics with Streptomyces: from in vivo through in vitro to in silico

1999

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Section: Figmentioning

confidence: 99%

Section: The In Vivo Yearsmentioning

confidence: 99%

Forty years of genetics with Streptomyces: from in vivo through in vitro to in silico

1999

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“…Four of these (R4, Hau3, TG1, and SV1) had been isolated previously for use as genetic tools for different Streptomyces spp. (13)(14)(15)(16). The remaining four, ELB20, Zemlya, Lika, and Sujidade, were isolated for this study.…”

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confidence: 99%

Evolutionary Relationships among Actinophages and a Putative Adaptation for Growth in Streptomyces spp

et al. 2013

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eThe genome sequences of eight Streptomyces phages are presented, four of which were isolated for this study. Phages R4, TG1, Hau3, and SV1 were isolated previously and have been exploited as tools for understanding and genetically manipulating Streptomyces spp. We also extracted five apparently intact prophages from recent Streptomyces spp. genome projects and, together with six phage genomes in the database, we analyzed all 19 Streptomyces phage genomes with a view to understanding their relationships to each other and to other actinophages, particularly the mycobacteriophages. Fifteen of the Streptomyces phages group into four clusters of related genomes. Although the R4-like phages do not share nucleotide sequence similarity with other phages, they clearly have common ancestry with cluster A mycobacteriophages, sharing many protein homologues, common gene syntenies, and similar repressor-stoperator regulatory systems. The R4-like phage Hau3 and the prophage StrepC.1 (from Streptomyces sp. strain C) appear to have hijacked a unique adaptation of the streptomycetes, i.e., use of the rare UUA codon, to control translation of the essential phage protein, the terminase. The Streptomyces venezuelae generalized transducing phage SV1 was used to predict the presence of other generalized transducing phages for different Streptomyces species. Bacteriophages are the most abundant and diverse genetic entities on planet Earth. Despite this, it has been proposed that all double-stranded DNA (dsDNA) phages share the same gene pool and that there are clear examples where common ancestry is evident between orthologous gene pairs from very different phages (for example, the capsid-encoding genes from coliphage HK97 and Streptomyces phage C31) (1, 2). Understanding the mechanisms through which phages generate such vast diversity is still a challenge, but the drivers of selection and adaptation to growth in different bacterial hosts are clearly strong influences. We are interested in the adaptations of phages that infect the Actinobacteria, a class of high-GC, Gram-positive bacteria that have very diverse morphology, physiology, and growth properties.Actinobacteria, particularly those in the genus Streptomyces, have a remarkable capacity to synthesize a diverse array of secondary metabolites of all the major classes. Indeed, 70% of clinically useful antibiotics come from Streptomyces spp. Genetic tools derived from Streptomyces phages have been invaluable in investigations aimed at understanding the fundamental biology of these bacteria and in manipulating antibiotic pathways (3, 4). Phages were used initially as cloning vectors, but since the early 1990s, integrating plasmid vectors based on phage-encoded site-specific recombination systems have been widely used, enabling the stable insertion of gene constructs into a defined site (attB) in the chromosome (5, 6). Moreover, as streptomycetes are mycelial and undergo a developmental cycle, phages that infect this group of bacteria are likely to have undergone host-specific evolu...

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“…lividans TK24 (Hopwood et al, 1985). High-titre lysates of KC301 were prepared by the method of Stuttard (1979) and stored at 4 "C.…”

Section: Methodsmentioning

confidence: 99%

The fate of introduced streptomycetes, plasmid and phage populations in a dynamic soil system

Cresswell¹,

Herron²,

Saunders³

et al. 1992

Journal of General Microbiology

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Populations of Sfrepfomyces l i v i h s and S. violuceolufus were monitored in natural soil amended with nutrients. The fates of a multicopy plasmid pIJ673, and an actinophage, KC301, were determined and the extent of gene transfer was estimated. The soil was incubated for 60 d during which time 'spent' soil was periodically removed followed by addition of fresh, uninoculated soil. Maximum numbers of bacteria and phage inoculants occurred at 15 d; this correlated with a peak in the amount of plasmid DNA detected and total numbers of transconjugants recovered. A KC301 lysogen of S . l i v i h s was also recovered at this time. Plasmid DNA was monitored by two methods, bead-beating and SDS/heat lysis; the latter was specific for mycelium while the former released DNA from spores and mycelium. Southern blots of soil DNA only showed the presence of plasmid DNA in SDS/heat lysis extracts from 15 d and 17 d samples, whereas positive signals were obtained throughout the experiment from bead-beaten extracts. The results confirmed that a well-developed mycelium was necessary for conjugation, phage infection, multiplication and lysogeny in soil.

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Transduction of Auxotrophic Markers in a Chloramphenicol-producing Strain of Streptomyces

Cited by 50 publications

References 15 publications

Forty years of genetics with Streptomyces: from in vivo through in vitro to in silico

Forty years of genetics with Streptomyces: from in vivo through in vitro to in silico

Evolutionary Relationships among Actinophages and a Putative Adaptation for Growth in Streptomyces spp

The fate of introduced streptomycetes, plasmid and phage populations in a dynamic soil system

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