Forty years of genetics with Streptomyces: from in vivo through in vitro to in silico

Hopwood, David A.

doi:10.1099/00221287-145-9-2183

Cited by 247 publications

(176 citation statements)

References 142 publications

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“…Housekeeping genes are mainly found in the central region of the linear chromosome, whereas nonessential functions are often located near its ends. 19 It is in this region where genes coding for secondary metabolites or transposons are located. 72 The ability to produce secondary metabolites, including antibiotics and bioactive compounds, is one of the most interesting properties of Streptomyces.…”

Section: Streptomycesmentioning

confidence: 99%

“…The possible mechanisms have been studied primarily in S. coelicolor, by far one of the better known biological models. 19,99 One of these mechanisms involves the PTS described above. 100,101 In S. coelicolor, there are at least four PTS transporters, all of which use the universal PTS energy-coupling enzymes enzyme I (EI, encoded by ptsI) and HPr (encoded by ptsH).…”

Section: Streptomycesmentioning

confidence: 99%

“…In the latter case, 50-80 kb of DNA are required for the modular poliketyde synthetases (PKSs) themselves. 19 Clusters of fungal biosynthetic genes have also been found for biosynthetic processes of sterigmatocystin formation by Aspergillus nidulans. 20 Antibiotic formation usually occurs during the late growth phase of the producing microorganism.…”

Section: Introductionmentioning

confidence: 99%

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Carbon source regulation of antibiotic production

et al. 2010

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Section: Streptomycesmentioning

confidence: 99%

Section: Streptomycesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Carbon source regulation of antibiotic production

et al. 2010

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“…Eventually, the aerial hyphae become subdivided into uninucleoid cells that develop into chains of hydrophobic spores (10). One of the striking features of streptomycetes and other actinomycetes is their ability to produce a wide variety of secondary metabolites, including many antibiotics, which are produced at about the same time as the onset of morphological differentiation in surface-grown cultures (19,31).…”

mentioning

confidence: 99%

ssgA Is Essential for Sporulation of Streptomyces coelicolor A3(2) and Affects Hyphal Development by Stimulating Septum Formation

et al. 2000

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The role of ssgA in cell division and development of streptomycetes was analyzed. An ssgA null mutant of Streptomyces coelicolor produced aerial hyphae but failed to sporulate, and ssgA can therefore be regarded as a novel whi gene. In addition to the morphological changes, antibiotic production was also disturbed, with strongly reduced actinorhodin production. These defects could be complemented by plasmid-borne ssgA. In the wild-type strain, transcription of ssgA was induced by nutritional shift-down and was shown to be linked to that of the upstream-located gene ssgR, which belongs to the family of iclR-type transcriptional regulator genes. Analysis of mycelium harvested from liquid-grown cultures by transmission electron microscopy showed that septum formation had strongly increased in ssgA-overexpressing strains in comparison to wild-type S. coelicolor and that spore-like compartments were produced at high frequency. Furthermore, the hyphae were significantly wider and contained irregular and often extremely thick septa. These data underline the important role for ssgA in Streptomyces cell division.

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“…Artificially circularized SCP1 at 621-284 635 bp by an aac (3) , 1999;Huang et al, 2003;Xu et al, 2006a;Zhang et al, 2009), while rep (hypothetical protein)/non-coding sequences (ncs) have been identified in pSLA2-L, pSCL2, pRL2, pRL4 and pFRL2 (Hiratsu et al, 2000;Wu et al, 2006;Zhang et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Three functional replication origins of the linear and artificially circularized plasmid SCP1 of Streptomyces coelicolor

et al. 2013

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Previous reports showed that the large linear plasmid SCP1 of Streptomyces coelicolor A3(2) contains a 5.4 kb centrally located replication locus. We report here that SCP1 actually contains three internal replication loci. Subcloning of the 5.4 kb sequence identified a 3.2 kb minimal locus (rep1A/repB/iteron) that determined propagation in Streptomyces lividans. The two newly identified replication genes, rep2A and rep3A, resembled the rep gene of Streptomyces circular plasmid pZL12. Transcription start points of the three replication genes were determined. The three replication loci could independently determine propagation in linear mode in S. lividans. Individual and sequential deletions of the rep1A and rep3A genes were successful. The SCP1-derived linear plasmids with deletions of the rep1A and/or rep3A genes still propagated in similar copy numbers, were inherited largely stable and were transferred efficiently by conjugation in S. coelicolor. Interestingly, SCP1 can be artificially circularized to yield a 280 kb circular plasmid, circular SCP-1 (C-SCP1), which contains the three replication loci. Strikingly, the copy numbers, inheritance and transfer of C-SCP1 resembled that of the linear SCP1 plasmids. Transcripts of the rep1A, rep2A and rep3A genes in linear or artificially circularized SCP1 were detected at all the time points of strain growth.

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Forty years of genetics with Streptomyces: from in vivo through in vitro to in silico

Cited by 247 publications

References 142 publications

Carbon source regulation of antibiotic production

Carbon source regulation of antibiotic production

ssgA Is Essential for Sporulation of Streptomyces coelicolor A3(2) and Affects Hyphal Development by Stimulating Septum Formation

Three functional replication origins of the linear and artificially circularized plasmid SCP1 of Streptomyces coelicolor

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