Transcriptomics identifies differences between ultrapure non-dioxin-like polychlorinated biphenyls (PCBs) and dioxin-like PCB126 in cultured peripheral blood mononuclear cells

Wens, Britt; Boever, Patrick De; Maes, Margaret E.; Hollanders, Karolien; Schoeters, Greet

doi:10.1016/j.tox.2011.06.004

Cited by 12 publications

(13 citation statements)

References 52 publications

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“…The aryl hydrocarbon receptor repressor (AhRR) and CYP1A1 and CYP1B1 were significantly upregulated in our experiment (Table 1). This is in line with the results from previous in vitro experiments (Wens et al, 2011). The CYP genes are involved in the cellular response to xenobiotic exposure and are also known to modulate the estrogen metabolism (Boelsterli, 2007).…”

Section: Tablesupporting

confidence: 89%

“…When PBMCs were exposed to BPA and PFOA, no genes from the known nuclear receptor pathways were found to be significantly changed in expression. The number of DEGs that could be related to nuclear receptors was rather limited, but this has been seen in other experiments and can be partly attributed to the resting state of the peripheral mononuclear blood cells (Wens et al, 2011).…”

Section: Tablementioning

confidence: 71%

“…This study was set up as a screening experiment to find compound-specific gene expression profiles and search for obesity-related gene expression changes. We have used the Agilent two-color microarray technology and associated protocols that have been proven to be reproducible and in good agreement with other microarray platforms and real-time PCR (Verstraelen et al, 2008;Wens et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…A low reactivity compared to other in vitro experiments may be attributed to the resting state of the PBMCs. Nevertheless, recent studies have shown that relevant gene expression changes can be obtained in resting PBMCs after 18 and 24 h of chemical exposure (Hochstenbach et al, 2010;Wens et al, 2011).…”

Section: Tablementioning

confidence: 99%

“…Peripheral blood cells sentinel changes in tissues and body fluids, and alter their transcriptome after exposure to environmental pollutants (Wens et al, 2011;Umbright et al, 2010;Burczynski and Dorner, 2006;Mohr and Liew, 2007). We hypothesized that these gene expression changes could be associated with known molecular mechanisms, and potentially metabolic disorders and obesity development.…”

Section: Introductionmentioning

confidence: 99%

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Cultured human peripheral blood mononuclear cells alter their gene expression when challenged with endocrine-disrupting chemicals

et al. 2013

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Section: Tablesupporting

confidence: 89%

Section: Tablementioning

confidence: 71%

Section: Discussionmentioning

confidence: 99%

Section: Tablementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cultured human peripheral blood mononuclear cells alter their gene expression when challenged with endocrine-disrupting chemicals

et al. 2013

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The influence of sex, genotype, and dose on serum and hippocampal cytokine levels in juvenile mice developmentally exposed to a human-relevant mixture of polychlorinated biphenyls

Matelski

Stietz

Sethi

et al. 2020

Current Research in Toxicology

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Polychlorinated biphenyls (PCBs) are pervasive environmental contaminants implicated as risk factors for neurodevelopmental disorders (NDDs). Immune dysregulation is another NDD risk factor, and developmental PCB exposures are associated with early life immune dysregulation. Studies of the immunomodulatory effects of PCBs have focused on the higher-chlorinated congeners found in legacy commercial mixtures. Comparatively little is known about the immune effects of contemporary, lower-chlorinated PCBs. This is a critical data gap given recent reports that lower-chlorinated congeners comprise >70% of the total PCB burden in serum of pregnant women enrolled in the MARBLES study who are at increased risk for having a child with an NDD. To examine the influence of PCBs, sex, and genotype on cytokine levels, mice were exposed throughout gestation and lactation to a PCB mixture in the maternal diet, which was based on the 12 most abundant PCBs in sera from MARBLES subjects. Using multiplex array, cytokines were quantified in the serum and hippocampus of weanling mice expressing either a human gain-of-function mutation in ryanodine receptor 1 (T4826I mice), a human CGG premutation repeat expansion in the fragile X mental retardation gene 1 (CGG mice), or both mutations (DM mice). Congenic wildtype (WT) mice were used as controls. There were dose-dependent effects of PCB exposure on cytokine concentrations in the serum but not hippocampus. Differential effects of genotype were observed in the serum and hippocampus. Hippocampal cytokines were consistently elevated in T4826I mice and also in WT animals for some cytokines compared to CGG and DM mice, while serum cytokines were usually elevated in the mutant genotypes compared to the WT group. Males had elevated levels of 19 cytokines in the serum and 4 in the hippocampus compared to females, but there were also interactions between sex and genotype for 7 hippocampal cytokines. Only the chemokine CCL5 in the serum showed an interaction between PCB dose, genotype, and sex. Collectively, these findings indicate differential influences of PCB exposure and genotype on cytokine levels in serum and hippocampal tissue of weanling mice. These results suggest that developmental PCB exposure has chronic effects on baseline serum, but not hippocampal, cytokine levels in juvenile mice.

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Gut microbiota dysbiosis induced by polychlorinated biphenyl 126 contributes to increased brain proinflammatory cytokines: Landscapes from the gut-brain axis and fecal microbiota transplantation

Tian

et al. 2022

Ecotoxicology and Environmental Safety

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Transcriptomics identifies differences between ultrapure non-dioxin-like polychlorinated biphenyls (PCBs) and dioxin-like PCB126 in cultured peripheral blood mononuclear cells

Cited by 12 publications

References 52 publications

Cultured human peripheral blood mononuclear cells alter their gene expression when challenged with endocrine-disrupting chemicals

Cultured human peripheral blood mononuclear cells alter their gene expression when challenged with endocrine-disrupting chemicals

The influence of sex, genotype, and dose on serum and hippocampal cytokine levels in juvenile mice developmentally exposed to a human-relevant mixture of polychlorinated biphenyls

Gut microbiota dysbiosis induced by polychlorinated biphenyl 126 contributes to increased brain proinflammatory cytokines: Landscapes from the gut-brain axis and fecal microbiota transplantation

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