Transcriptomics and Metabolomics Analysis of Sclerotium rolfsii Fermented with Differential Carbon Sources

Abstract

Scleroglucan is obtained from Sclerotium rolfsii and is widely used in many fields. In this study, transcriptomics combined with metabolomics were used to study the global metabolites and gene changes. The results of the joint analysis showed that the DEGs (differentially expressed genes) and DEMs (differentially expressed metabolites) of SEPS_48 (fermented with sucrose as a carbon source for 48 h) and GEPS_48 (fermented with glucose as a carbon source for 48 h) comparison groups were mainly related to cell metabolism, focusing on carbohydrate metabolism, amino acid metabolism, and amino sugar and nucleoside sugar metabolism. We therefore hypothesized that the significant differences in these metabolic processes were responsible for the differences in properties. Moreover, the joint analysis provides a scientific theoretical basis for fungal polysaccharides biosynthesis and provides new insights into the effects of carbon sources on the production. As an excellent bioenergy and biological product, scleroglucan can be better applied in different fields, such as the food industry.

De Novo RNA Sequencing and Transcriptome Analysis of Sclerotium rolfsii Gene Expression during Sclerotium Development

“…In this study, we successfully identified the potential genetic mechanisms underlying the pathology of S. rolfsii, which causes southern blight disease in numerous crops. Previously, Song et al [27] cultured S. rolfsii in sucrose and glucose media to investigate global…”

“…In this study, we successfully identified the potential genetic mechanisms underlying the pathology of S. rolfsii, which causes southern blight disease in numerous crops. Previously, Song et al [27] cultured S. rolfsii in sucrose and glucose media to investigate global metabolic and genetic changes using LC-MS combined with RNA-Seq. Furthermore, Schmid applied massively parallel short-read 454 pyrosequencing to identify DEGs under scleroglucan-producing and non-producing conditions [21].…”