Transcriptomics Analysis of Primordium Formation in Pleurotus eryngii

Ye, Dou; Du, Fang; Zou, Yonggen; Hu, Qingxiu

doi:10.3390/genes12121863

Cited by 4 publications

(5 citation statements)

References 42 publications

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“…Similar to common edible mushrooms, the nutritional and reproductive growth of morels show very different features and dynamics (Figures 1a and 6). Primordium formation is considered to be a critical "intermediate" or "transitional" phase for successful differentiation into ascocarps [58][59][60]. Our results support that primordium formation is triggered by changing comprehensive environmental information (e.g., nutrient availability, temperature, light, humidity, and oxygen) and involves a transition from nutritional mycelia to primordia, which differentiate into reproductive complex structures.…”

Section: Discussionsupporting

confidence: 78%

Integration of Metabolomes and Transcriptomes Provides Insights into Morphogenesis and Maturation in Morchella sextelata

Zhang,

Shi,

Zhang

et al. 2023

JoF

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True morels (Morchella, Pezizales) are a popular edible and medicinal fungus with great nutritional and economic value. The dynamics and regulatory mechanisms during the morphogenesis and maturation of morels are poorly understood. In this study, the metabolomes and transcriptomes of the mycelium (MY), primordium differentiation (PR), young fruiting body (YFB), and mature fruiting body (MFB) were comprehensively analyzed to reveal the mechanism of the morphogenesis and maturation of Morchella sextelata. A total of 748 differentially expressed metabolites (DEMs) and 5342 differentially expressed genes (DEGs) were detected, mainly enriched in the carbohydrate, amino acid, and lipid metabolism pathways, with the transition from the mycelium to the primordium being the most drastic stage at both the metabolic and transcriptional levels. The integrated metabolomics and transcriptomics highlighted significant correlations between the DEMs and DEGs, and specific amino acid and nucleotide metabolic pathways were significantly co-enriched, which may play key roles in morphological development and ascocarp maturation. A conceptual model of transcriptional and metabolic regulation was proposed during morphogenesis and maturation in M. sextelata for the first time, in which environmental factors activate the regulation of transcription factors, which then promote metabolic and transcriptional regulation from vegetative to reproductive growth. These results provide insights into the metabolic dynamics and transcriptional regulation during the morphogenesis and maturation of morels and valuable resources for future breeding enhancement and sustainable artificial cultivation.

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Section: Discussionsupporting

confidence: 78%

Integration of Metabolomes and Transcriptomes Provides Insights into Morphogenesis and Maturation in Morchella sextelata

Zhang,

Shi,

Zhang

et al. 2023

JoF

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“…The growth and development of edible fungi are generally divided into two stages, vegetative growth and reproductive growth, among which the formation of primordium is an important and sensitive stage in the transition from vegetative growth to reproductive growth [ 23 ]. In this stage, those mycelia twisted together to form a mycelial community, forming a mass of undifferentiated protoplasm tissue called the primordium or fruiting body [ 5 ]. In the mushroom-forming basidiomycete Schizophyllum commune , a regulator of G-protein signaling, Thn1, was involved in the development via pheromone signaling that affects physiological interactions [ 43 ].…”

Section: Discussionmentioning

confidence: 99%

“…P. eryngii is one of the most valuable edible mushroom species in China and other Asian countries because of its thick flesh, delicious taste, and medicinal value [ 2 ]. Previous studies have demonstrated that P. eryngii exhibited various biological activities, including antioxidant, immunomodulatory, bacteriostatic, and tumor-inhibitory effects [ 3 , 4 , 5 ]. Mushroom development of P. eryngii can be divided into three stages: hyphal knot, primordium, and fruiting body [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis Reveals the Function of a G-Protein α Subunit Gene in the Growth and Development of Pleurotus eryngii

Cao

Sun

et al. 2023

JoF

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Pleurotus eryngii is a commercially important edible fungus with high nutritional and economic value. However, few functional studies have examined key genes affecting the growth and development of P. eryngii. In this study, transformed strains, including over-expression (PeGNAI-OE) and RNA interference (PeGNAI-RNAi) lines, were constructed to elucidate the role of GNAI in P. eryngii growth. GNAI expression was found to affect the mycelial growth and the number of clamp connections. Moreover, the transformed strains were shown to have higher endogenous cAMP levels, thus affecting amylase and laccase activity. Fruiting experiments showed that GNAI expression revealed the formation of P. eryngii primordia and the number of buttons, while transcription analysis identified GNAI gene involvement in the growth and development of P. eryngii. Seven downstream genes regulated by GNAI were differentially expressed in PeGNAI-OE and PeGNAI-RNAi compared to wild type (WT). These genes may be related to mycelial growth and enzyme activity. They were involved in the MAPK signaling pathway, inositol phosphate metabolism, ascorbate, aldarate metabolism, and starch and sucrose metabolism. In summary, GNAI performs different physiological functions in regulating the growth and development of P. eryngii. Importantly, the molecular mechanisms of GNAI regulatory function are relatively complex and need further study.

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“…A recent transcriptome analysis is also available for Pleurotus eryngii (kings trumpet), a close relative of P. ostreatus [ 20 ]. A comparison of transcriptomics of mature mycelia and primordia showed a total of 10,718 upregulated and 8937 downregulated genes in the primordium library.…”

Section: From Mycelium To Fruiting Body: Differential Gene Expressionmentioning

confidence: 99%

Mycelium vs. Fruiting Bodies of Edible Fungi—A Comparison of Metabolites

et al. 2022

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Edible mushrooms are widely appreciated for their appealing flavours, low caloric values and high content of presumably health-protecting metabolites. Their long history of safe use together with the looming worldwide food crisis have revived the idea of generating meat analogues and protein isolates by the controlled fermentation of mycelia of these edible fungi as a dietary option. The occurrence of proteins, polysaccharides, smaller metabolites, metal ions and toxins in mycelia and fruiting bodies is compared among the three most popular species, Agaricus bisporus (button mushroom), Pleurotus ostreatus (oyster mushroom), Lentinus edodes (shiitake) and some closely related species. Large effects of substrate chemistry, strain, developmental stage and ecological interactions result in a wide variation of the concentrations of some metabolites in both mycelial cells and fruiting bodies. This is obviously a result of the high adaptation abilities required to survive in natural habitats. Fungal bioprocesses are decoupled from agricultural production and can be operated anytime, anywhere, and on any scale according to demand. It is concluded that fungal biomass, if produced under food-grade conditions and on an industrial scale, could provide a safe and nutritious meat substitute and protein isolates with a high biological value for future vegan foods.

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Transcriptomics Analysis of Primordium Formation in Pleurotus eryngii

Cited by 4 publications

References 42 publications

Integration of Metabolomes and Transcriptomes Provides Insights into Morphogenesis and Maturation in Morchella sextelata

Integration of Metabolomes and Transcriptomes Provides Insights into Morphogenesis and Maturation in Morchella sextelata

Transcriptome Analysis Reveals the Function of a G-Protein α Subunit Gene in the Growth and Development of Pleurotus eryngii

Mycelium vs. Fruiting Bodies of Edible Fungi—A Comparison of Metabolites

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