Transcriptomic profiling of sorghum leaves and roots responsive to drought stress at the seedling stage

Zhang, Dengfeng; Zeng, Tingru; Liu, Xu-yang; Gao, Chenxi; Li, Yongxiang; Li, Chunhui; Song, Yifu; Shi, Yunsu; Wang, Tianyu; Liu, Yu

doi:10.1016/s2095-3119(18)62119-7

Cited by 26 publications

(26 citation statements)

References 41 publications

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“…Other remarkable observations in the study [ 90 ] relate to a shift in hormonal balance during drought and recovery from stress. For example, DEGs involved in ABA biosynthesis and catabolism were up-regulated in roots during drought, but down-regulated during recovery from stress, with an inverse expression pattern of DEGs involved in the auxin signal pathway.…”

Section: Sorghum Transcriptomics Studies In Response To Drought Stressmentioning

confidence: 96%

“…Transcriptomic studies of sorghum plants under drought stress have also used a broad spectrum of RNA profiling techniques, plant tissues, experimental designs and water stress treatments [ 19 , 73 , 74 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 , 94 ], as summarized in Table 2 . Earlier studies investigated a dehydrin mRNA expression pattern in drought-stressed sorghum tissues using Northern blotting and hybridization methods against a 32 P labeled maize dehydrin cDNA probe [ 85 , 86 ].…”

Section: Sorghum Transcriptomics Studies In Response To Drought Stressmentioning

confidence: 99%

“…Similarly, Zhang and co-workers [ 90 ] investigated the transcriptomic profiling of leaves and roots of a drought-tolerant sorghum variety XGL-1 in response to drought stress using RNA-seq analysis. Sorghum seedlings were exposed to either mild or severe water deprivation for 7 days before re-watering some for an additional 2 days.…”

Section: Sorghum Transcriptomics Studies In Response To Drought Stressmentioning

confidence: 99%

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Sorghum’s Whole-Plant Transcriptome and Proteome Responses to Drought Stress: A Review

Ngara

Goche

Swanevelder

et al. 2021

Life

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Sorghum is a cereal crop with key agronomic traits of drought and heat stress tolerance, making it an ideal food and industrial commodity for hotter and more arid climates. These stress tolerances also present a useful scientific resource for studying the molecular basis for environmental resilience. Here we provide an extensive review of current transcriptome and proteome works conducted with laboratory, greenhouse, or field-grown sorghum plants exposed to drought, osmotic stress, or treated with the drought stress-regulatory phytohormone, abscisic acid. Large datasets from these studies reveal changes in gene/protein expression across diverse signaling and metabolic pathways. Together, the emerging patterns from these datasets reveal that the overall functional classes of stress-responsive genes/proteins within sorghum are similar to those observed in equivalent studies of other drought-sensitive model species. This highlights a monumental challenge of distinguishing key regulatory genes/proteins, with a primary role in sorghum adaptation to drought, from genes/proteins that change in expression because of stress. Finally, we discuss possible options for taking the research forward. Successful exploitation of sorghum research for implementation in other crops may be critical in establishing climate-resilient agriculture for future food security.

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Section: Sorghum Transcriptomics Studies In Response To Drought Stressmentioning

confidence: 96%

Section: Sorghum Transcriptomics Studies In Response To Drought Stressmentioning

confidence: 99%

Section: Sorghum Transcriptomics Studies In Response To Drought Stressmentioning

confidence: 99%

See 1 more Smart Citation

Sorghum’s Whole-Plant Transcriptome and Proteome Responses to Drought Stress: A Review

Ngara

Goche

Swanevelder

et al. 2021

Life

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“…The molecular basis of plant adaptation to water scarcity conditions is complex. Transcriptomic analyses of plants under water-deficit stress have identified numerous candidate genes [ 19 , 20 , 21 , 22 ]. According to their putative functions, these drought-related genes can be divided into two groups encoding functional proteins and regulatory proteins [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Gene Expression Profiles Analysis Reveal Novel Insights into Drought Stress in Foxtail Millet (Setaria italica L.)

Qin

Chen

et al. 2020

IJMS

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Foxtail millet (Setaria italica (L.) P. Beauv) is an important food and forage crop because of its health benefits and adaptation to drought stress; however, reports of transcriptomic analysis of genes responding to re-watering after drought stress in foxtail millet are rare. The present study evaluated physiological parameters, such as proline content, p5cs enzyme activity, anti-oxidation enzyme activities, and investigated gene expression patterns using RNA sequencing of the drought-tolerant foxtail millet variety (Jigu 16) treated with drought stress and rehydration. The results indicated that drought stress-responsive genes were related to many multiple metabolic processes, such as photosynthesis, signal transduction, phenylpropanoid biosynthesis, starch and sucrose metabolism, and osmotic adjustment. Furthermore, the Δ1-pyrroline-5-carboxylate synthetase genes, SiP5CS1 and SiP5CS2, were remarkably upregulated in foxtail millet under drought stress conditions. Foxtail millet can also recover well on rehydration after drought stress through gene regulation. Our data demonstrate that recovery on rehydration primarily involves proline metabolism, sugar metabolism, hormone signal transduction, water transport, and detoxification, plus reversal of the expression direction of most drought-responsive genes. Our results provided a detailed description of the comparative transcriptome response of foxtail millet variety Jigu 16 under drought and rehydration environments. Furthermore, we identify SiP5CS2 as an important gene likely involved in the drought tolerance of foxtail millet.

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“…However, filling stage is the most important period for yield production, at which drought can lead to severe decline of yield in sorghum [28]. Sorghum can feed at least 5 million people in these areas due to its unique drought adaptability [29][30][31], and sorghum roots play an important role in its drought tolerance [32]. Wang et al reported that a higher root activity has been found in the stay green sorghum B35 as compared to non-green-stayed sorghum Sanchisan and is considered as one of the drought-resistant mechanisms under drought conditions [33].…”

Section: Introductionmentioning

confidence: 99%

The Role of Deep Roots in Sorghum Yield Production under Drought Conditions

Chen

Gao

et al. 2020

Agronomy

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Root function plays a vital role in maintaining crop production. However, the role of deep roots in yield production and their effects on photosynthetic performance in sorghum remain unclear. This study aimed to provide theoretical supports for establishing highly efficient root systems of sorghum to achieve more yield under certain conditions. In this study, two sorghum (Sorghum bicolor L. Moench) cultivars, Jiza127 and Jiza305, were cultivated in soil columns as experimental materials. Three treatments (no roots removed, CK; roots removed at 30 cm underground, R30; roots removed at 60 cm underground, R60) were carried out under drought conditions during the filling stage. The root bleeding intensity, endogenous substances in the root bleeding sap, photosynthetic characteristics, dry matter accumulation, and yield were measured. The results showed that R30 and R60 significantly reduced yield in both sorghum cultivars, and the effect of R30 on yield was greater than that of R60. The contributions of roots below 30 cm to the yield of both sorghum hybrids were notably higher than those below 60 cm. R30 significantly reduced the dry matter weights (DMWs) of leaves, stems, sheaths, and panicles. R60 significantly reduced the DMW of panicles but had no significant effect on the DMWs of leaves and stems. R30 significantly reduced the photosynthetic level and PSII reaction center activity; however, the effect of R60 was not significant. Although both R30 and R60 significantly reduced root activity and the soluble sugar, amino acid, gibberellin (GA3), and abscisic acid (ABA) contents of the root bleeding sap, some of the above indicators in R60 were significantly higher than those in R30 during the filling stage, indicating that the deeper roots (below 30 cm) had a critical regulatory effect on the physiological processes of the aerial parts in sorghum, which resulted in a stronger effect on yield, especially under drought conditions. In brief, the deep roots of sorghum played a key role in yield production, but the roots in different soil depths regulated yield production in different ways. Our results indicate that deep roots of sorghum deserve consideration as a potential trait for yield improvement especially under drought conditions.

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Transcriptomic profiling of sorghum leaves and roots responsive to drought stress at the seedling stage

Cited by 26 publications

References 41 publications

Sorghum’s Whole-Plant Transcriptome and Proteome Responses to Drought Stress: A Review

Sorghum’s Whole-Plant Transcriptome and Proteome Responses to Drought Stress: A Review

Genome-Wide Gene Expression Profiles Analysis Reveal Novel Insights into Drought Stress in Foxtail Millet (Setaria italica L.)

The Role of Deep Roots in Sorghum Yield Production under Drought Conditions

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