Transcriptomic Profile of Canine Mammary Ductal Carcinoma

Abstract

Dogs can be excellent models for spontaneous studies about breast cancers, presenting similarities in clinical behavior and molecular pathways of the disease. Thus, analyses of the canine transcriptome can identify deregulated genes and pathways, contributing to the identification of biomarkers and new therapeutic targets, benefiting humans and animals. In this context, this study aimed to determine the transcriptional profile of canine mammary ductal carcinoma and contribute to the clarification of the importance of deregulated molecules in the molecular pathways involved in the disease. Therefore, we used mammary ductal carcinoma tissue samples and non-tumor mammary tissue from the radical mastectomy of six female dogs. Sequencing was performed on the NextSeq-500 System platform. A comparison of carcinoma tissue and normal tissue revealed 633 downregulated and 573 upregulated genes, which were able to differentiate the groups by principal component analysis. Gene ontology analysis indicated that inflammatory, cell differentiation and adhesion, and extracellular matrix maintenance pathways were mainly deregulated in this series. The main differentially expressed genes observed in this research can indicate greater disease aggressiveness and worse prognosis. Finally, the study of the canine transcriptome indicates that it is an excellent model to generate information relevant to oncology in both species.

Halofuginone-guided nano-local therapy: Nano-thermosensitive hydrogels for postoperative metastatic canine mammary carcinoma with scar removal

“…The most common tumors in middle-age and elderly female dogs are canine mammary gland tumors ( Santos et al, 2023 ). The malignancy conversion rate of these tumors is approximately 50%.…”

Studies on the association of malondialdehyde as a biomarker for oxidative stress and degree of malignancy in dogs with mammary adenocarcinomas

“…According to previous literature, at least 40–50% of these tumours appear to be malignant (Sorenmo, 2003 ). There is a growing interest in the investigation of pathogenesis and prognostic biomarkers for the assessment of malignancy (Burrai et al., 2022 ; Estaller et al., 2022 ; Michishita et al., 2011 ; Montalli et al., 2017 ; Rasotto et al., 2017 ; Santos et al., 2023 ). The aetiopathogenesis of tumours of the mammary gland in dogs is not yet fully understood, although genetic, nutritional and hormonal factors are discussed (Sarli et al., 2002 ; Sorenmo, 2003 ).…”