Transcriptomic information from Pacific white shrimp ( Litopenaeus vannamei ) ovary and eyestalk, and expression patterns for genes putatively involved in the reproductive process

Ventura-López, Claudia; Galindo-Torres, Pavel; Arcos, Fabiola G.; Galindo-Sánchez, Clara E.; Racotta, Ilie S.; Escobedo‐Fregoso, Cristina; Llera-Herrera, Raúl; Ibarra, Ana M.

doi:10.1016/j.ygcen.2016.12.005

Cited by 18 publications

(12 citation statements)

References 128 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A recent study pointed out the importance of endogenous Vg/Vn in L . vannamei ovarian development [ 52 ], and our current study showed that exogenous Vg/Vn play the same role in this process. Our study also provided new evidence that hepatopancreatic primary cell culture is an applicable method for testing the regulators of Vg/Vn synthesis in L .…”

Section: Discussionsupporting

confidence: 57%

Mechanisms for type-II vitellogenesis-inhibiting hormone suppression of vitellogenin transcription in shrimp hepatopancreas: Crosstalk of GC/cGMP pathway with different MAPK-dependent cascades

et al. 2018

View full text Add to dashboard Cite

Vitellogenesis is the process of yolk formation via accumulating vitellin (Vn) with nutrients in the oocytes. Expression of vitellogenin (Vg), the precursor of Vn, is one of the indicators for the start of vitellogenesis. In Pacific white shrimp (Litopenaeus vannamei), the type-II vitellogenesis-inhibiting hormone (VIH-2) effectively suppresses hepatopancreatic Vg mRNA expression. In this study, we demonstrate the increasing transcript levels of hepatopancreatic Vg during L. vannamei ovarian development, suggesting that the hepatopancreas-derived Vg/Vn may also contribute to vitellogenesis in this species. Using a combination of in vivo injections and in vitro primary cell cultures, we provide evidences that the inhibition of VIH-2 on hepatopancreatic Vg gene expression is mediated through a functional coupling of the GC/cGMP pathway with different MAPK-dependent cascades in female shrimp. In VIH-2 signaling, the NO-independent GC/cGMP/PKG cascades were upstream of the MAPKs. Activations of the MAPK signal by VIH-2 include the phosphorylation of JNK and the mRNA/protein expression of P38MAPK. Additionally, the cAMP/PKA pathway is another positive intracellular signal for hepatopancreatic Vg mRNA expression but is independent of its VIH-2 regulation. Our findings establish a model for the signal transduction mechanism of Vg regulation by VIH and shed light on the biological functions and signaling of the CHH family in crustaceans.

show abstract

Section: Discussionsupporting

confidence: 57%

Mechanisms for type-II vitellogenesis-inhibiting hormone suppression of vitellogenin transcription in shrimp hepatopancreas: Crosstalk of GC/cGMP pathway with different MAPK-dependent cascades

et al. 2018

View full text Add to dashboard Cite

show abstract

“…IGFBP-4 might play an essential role in the differentiation of hemocytes from Hem 1 to Hem 2 in shrimp. The high expression of IGFBP is determined in the brain and gonads of L. vannamei ( Ventura-López et al, 2017 ). This fact also suggests that IGFBP plays a possible role in organ growth and maturation in shrimp.…”

Section: Resultsmentioning

confidence: 99%

Single-cell RNA-seq analysis reveals penaeid shrimp hemocyte subpopulations and cell differentiation process

Koiwai

Koyama

Tsuda³

et al. 2021

eLife

View full text Add to dashboard Cite

Crustacean aquaculture is expected to be a major source of fishery commodities in the near future. Hemocytes are key players of the immune system in shrimps; however, their classification, maturation, and differentiation are still under debate. To date, only discrete and inconsistent information on the classification of shrimp hemocytes has been reported, showing that the morphological characteristics are not sufficient to resolve their actual roles. Our present study using single-cell RNA sequencing, revealed six types of hemocytes of Marsupenaeus japonicus based on their transcriptional profiles. We identified markers of each subpopulation and predicted the differentiation pathways involved in their maturation. We also predicted cell growth factors that might play crucial roles in hemocyte differentiation. Different immune roles among these subpopulations were suggested from the analysis of differentially expressed immune-related genes. These results provide a unified classification of shrimp hemocytes, which improves the understanding of its immune system.

show abstract

“…The synthesis of MF is inhibited by serotonin; therefore, this neurotransmitter was proposed as a neuroregulator of MF synthesis (Homola et al, 1989;Fingerman, 1997, for review). In L. vannamei, the key enzyme for MF synthesis: farnesoic acid O-methyl transferase was expressed in different tissues, and the highest expression was measured in the brain (Ventura-López et al, 2017).…”

Section: Reproductive Endocrinologymentioning

confidence: 99%

“…New candidate genes putatively involved in the control and regulation of gonad maturation have been identified in L. vannamei using a transcriptomic approach (Ventura-López et al, 2017), and in P. monodon using oligonucleotide microarray analysis (Brady et al, 2013). In L. vannamei, the authors reported a vitellogenin receptor gene (VTGR) with the highest expression in previtellogenic gonads.…”

Section: Reproductive Endocrinologymentioning

confidence: 99%

“…: vitellogenin receptor (vtgr), minichromosome maintenance (mcm) family members and membrane progestin receptor gamma (mprg) steroid receptor. Ventura-López et al, 2017 Penaeus monodon Transcriptome of ovaries and testes revealed transcripts related with important reproductive pathways, such as: gonadotropin-releasing hormone (GnRH) and progesterone-mediated oocyte maturation. These findings are helping to define strategies to minimize the P. monodon low capacity of reproduction in captivity.…”

Section: Litopenaeus Vannameimentioning

confidence: 99%

See 1 more Smart Citation

Research frontiers in penaeid shrimp reproduction: Future trends to improve commercial production

2019

View full text Add to dashboard Cite

Shrimp farming worldwide is based on a similar technological package, characterized by three phases: Controlled Reproduction, Larvae Culture, and Grow-out Culture. This basic aquaculture package uses broodstock animals, with different levels of fundamental genetic selection, induced to mature and reproduce based on unilateral eyestalk ablation. This review identified ten reproduction-related research subjects that can improve the shrimp industry based on basic scientific knowledge and four levels of application: eyestalk ablation alternatives, larvae production, product protection, and grow-out yield improvement. Species-specificity must be considered in developing biotechnology solutions. Alternatives to eyestalk ablation for controlling ovarian maturation are based on neurotransmitter regulation in Litopenaeus; however, the environmental impact of this approach has to be evaluated. Maturation by RNAi requires further evaluation, and maturation pheromones have not been explored. Sex reversal, hybridization, in vitro fertilization, and seedstock cryopreservation of penaeids require fundamental research. Triploid culture and genetic selection can be applied to some species for product protection; however, for L. vannamei, triploidization is not practical yet.

show abstract

Transcriptomic information from Pacific white shrimp ( Litopenaeus vannamei ) ovary and eyestalk, and expression patterns for genes putatively involved in the reproductive process

Cited by 18 publications

References 128 publications

Mechanisms for type-II vitellogenesis-inhibiting hormone suppression of vitellogenin transcription in shrimp hepatopancreas: Crosstalk of GC/cGMP pathway with different MAPK-dependent cascades

Mechanisms for type-II vitellogenesis-inhibiting hormone suppression of vitellogenin transcription in shrimp hepatopancreas: Crosstalk of GC/cGMP pathway with different MAPK-dependent cascades

Single-cell RNA-seq analysis reveals penaeid shrimp hemocyte subpopulations and cell differentiation process

Research frontiers in penaeid shrimp reproduction: Future trends to improve commercial production

Contact Info

Product

Resources

About