Transcriptomic changes in the pre-parasitic juveniles of Meloidogyne incognita induced by silencing of effectors Mi-msp-1 and Mi-msp-20

Somvanshi, Vishal Singh; Phani, Victor; Banakar, Prakash; Chatterjee, Madhurima; Budhwar, Roli; Shukla, Rohit Nandan; Rao, Uma

doi:10.1007/s13205-020-02353-8

Cited by 3 publications

(6 citation statements)

References 51 publications

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“…Advancement in the genomics can be applied to the RKN control in eggplant. In this direction, silencing Mi-msp-20, on the other side, culminated in 409 transcripts being differentially regulated in M. incognita J2s compared to 29 in Mi-msp-1 silenced J2s (Somvanshi et al, 2020). A fascinating discovery was that silenced Mi-msp-1 and Mi-msp-20 nematodes shared 5.9% of the up-regulated transcripts, suggesting that there is no crosstalk between the two effectors.…”

Section: Classical Breeding Techniques To Modern Toolsmentioning

confidence: 99%

“…Further, it has been reported that it is nearly impossible to attain total nematode resistance (Van Esse et al, 2020). In M. incognita, the Mi-msp-1 HIGS impact is thought to be universal, spreading across the nematode body after picking up dsRNA/siRNA molecules through the stylet orifice (Joshi et al, 2020;Somvanshi et al, 2020). Furthermore, the RNAi effect was sustained until nematode maturity, suggesting that M. incognita offspring with Mi-msp-1 could be formed (Nguyen, 2016).…”

Section: Classical Breeding Techniques To Modern Toolsmentioning

confidence: 99%

“…Both genes have also been shown to interfere with other cell-wall degrading enzymes (CWDEs) (Haegeman et al, 2013;Jones et al, 2013). In eggplants, Mi-msp-1 HIGS decreased root galling, the number of eggs per egg mass by 36.08-41.20%, and the nematode multiplication factor by 62.37-70.62 % (Chaudhary et al, 2019a;Somvanshi et al, 2020).…”

Section: Classical Breeding Techniques To Modern Toolsmentioning

confidence: 99%

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Breeding for Root-Knot Nematode Resistance in Eggplant: Progress and Prospects

Sharma¹,

Kaushik²

2021

Preprint

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Eggplant is a functional food owing to its anti-diabetic, anti-inflammatory, and cardio-protective properties. Root-knot nematodes (RKN) are a threat to the successful production of eggplant. RKN infestation manifests as root damage, stunted development, and structural deformations of the plant. RKN infestation can be managed using a variety of management techniques like soil amendments and chemical treatments. Breeding for nematode tolerance is critical for high yields and stable results. As a result, breeding approaches are the most efficient and cost-effective nematode management methods. Furthermore, with advances in breeding technology and genomics assistance, it is becoming more feasible and straightforward. As a result, we've compiled a list of the most recent breeding developments for Meloidogyne spp. resistance in eggplant. We hope this information will serve as an important resource for the eggplant breeders.

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Section: Classical Breeding Techniques To Modern Toolsmentioning

confidence: 99%

Section: Classical Breeding Techniques To Modern Toolsmentioning

confidence: 99%

Section: Classical Breeding Techniques To Modern Toolsmentioning

confidence: 99%

See 1 more Smart Citation

Breeding for Root-Knot Nematode Resistance in Eggplant: Progress and Prospects

Sharma¹,

Kaushik²

2021

Preprint

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“…Other than cell wall composition, in M. incognita CWDEs expression is reported to be regulated by other effectors viz., Meloidogyne secretory protein (MSP)-1, 18, and 20. Silencing of these effectors showed transcriptional oscillation of various CWDEs which suggests the presence of effector-dependent retrograde signalling in RKNs (Shivakumara et al, 2016(Shivakumara et al, , 2017Chaudhary et al, 2019a;Somvanshi et al, 2020). The lack of knowledge about CWDE isoform dynamics is one of the major bottlenecks for not achieving complete control of RKN infection via silencing of CWDEs.…”

Section: Pcwdes Mediated Entry Of Rkns In the Rootsmentioning

confidence: 99%

“…Similarly, silencing of msp-20 in M. incognita had negative effects on PCWDEs expression. It also hampered nematode development, behaviour, and cellular physiology by disturbing various developmental signalling pathways ( Shivakumara et al, 2017 ; Somvanshi et al, 2020 ). This clearly suggests the pleiotropic effects of effector silencing on RKN fitness ( Somvanshi et al, 2020 ).…”

Section: Role Of Sub-ventral Oesophageal Glands In Early Infectionmentioning

confidence: 99%

Effectors of Root-Knot Nematodes: An Arsenal for Successful Parasitism

2021

Self Cite

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Root-knot nematodes (RKNs) are notorious plant-parasitic nematodes first recorded in 1855 in cucumber plants. They are microscopic, obligate endoparasites that cause severe losses in agriculture and horticulture. They evade plant immunity, hijack the plant cell cycle, and metabolism to modify healthy cells into giant cells (GCs) – RKN feeding sites. RKNs secrete various effector molecules which suppress the plant defence and tamper with plant cellular and molecular biology. These effectors originate mainly from sub-ventral and dorsal oesophageal glands. Recently, a few non-oesophageal gland secreted effectors have been discovered. Effectors are essential for the entry of RKNs in plants, subsequently formation and maintenance of the GCs during the parasitism. In the past two decades, advanced genomic and post-genomic techniques identified many effectors, out of which only a few are well characterized. In this review, we provide molecular and functional details of RKN effectors secreted during parasitism. We list the known effectors and pinpoint their molecular functions. Moreover, we attempt to provide a comprehensive insight into RKN effectors concerning their implications on overall plant and nematode biology. Since effectors are the primary and prime molecular weapons of RKNs to invade the plant, it is imperative to understand their intriguing and complex functions to design counter-strategies against RKN infection.

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