Transcriptomic and proteomic profiles of II YOU 838 (Oryza sativa) provide insights into heat stress tolerance in hybrid rice

Abstract

Heat stress is an increasing threat to rice production worldwide. To investigate the mechanisms of heat tolerance in hybrid rice and their contributions to rice heterosis, we compared the transcriptome of the hybrid rice II YOU 838 (II8) with the transcriptomes of its parents Fu Hui 838 (F8) and II-32A (II3) after heat stress at 42 °C for 0 h, 24 h, 72 h and 120 h. We also performed a proteomic analysis in II8 after heat stress at 42 °C for 24 h. The transcriptome data revealed time-dependent gene expression patterns under the heat stress conditions, and the heat stress response of II8 was greatly different from those of its parents. Gene ontology analysis of the differentially expressed genes that were clustered using k-means clustering showed that most of the up-regulated genes were involved in responses to stimuli, cell communication, and metabolic and transcription factor activities, whereas the down-regulated genes were enriched in photosynthesis and signal transduction. Moreover, 35 unique differentially abundant proteins, including a basic helix-loop-helix transcription factor (bHLH96), calmodulin-binding transcription activator, heat shock protein (Hsp70), and chaperonin 60 (CPN60), were detected in the proteomic analysis of II8 under heat stress. The co-regulatory analysis revealed novel genes and pathways involved in heat tolerance, namely, ferredoxin-NADP reductase, peroxidases, mitogen-activated protein kinase kinase kinase, and heat shock factor (HSF)–Hsp network. Members of the Hsp and HSF families had over-dominant expression patterns in the hybrid compared with its parents, to help maintain the higher photosynthesis and antioxidant defense systems in the hybrid. Our study suggests that the complex HSF–Hsp regulatory network contribute to the heat tolerance of the hybrid rice.

Jasmonates alleviate spikelet‐opening impairment caused by high temperature stress during anthesis of photo‐thermo‐sensitive genic male sterile rice lines

“…The finding that JAs could alleviate spikelet‐opening impairment caused by HT stress during anthesis of PTSGMS lines would have great significance in the production of “two‐line‐method” hybrid rice. Firstly, it is an important strategy to breed and select a heat tolerant rice cultivar or a line to cope with the increasing hazard of HT stress to rice production (Karwa et al., 2020; Luo & Lau, 2019; Wahab, Akkareddy, Shanthi, & Latha, 2020; Wang et al., 2020). A higher level of JA or MeJA in the lodicules under HT stress could be used as a criterion, at least as a physiological trait, for breeding and selecting a heat tolerant rice cultivar especially a PTSGMS rice line.…”

Can omics deliver temperature resilient ready-to-grow crops?

“…Transcriptomics includes the functional genome of living organisms dealing with the total number of transcripts, their abundance in a specific cell, and posttranscriptional modifications [90,136]. Transcriptomic studies have been conducted by several technologies, including RNA sequencing, a hybridization-based approach, and other sequencing applications (Table 2).…”

“…Transcriptome analysis of hybrid rice was conducted under HS (42 C) and revealed 4016, 3073, and 3596 deferentially expressed genes (DEGs) that are involved in metabolic and TF activities, regulate signal transduction and photosynthesis [90]. Clusters of genes were exhibited according to their expression, which mainly involved thermotolerance against HS.…”

“…Clusters of genes were exhibited according to their expression, which mainly involved thermotolerance against HS. Some genes were found to participate in acetylation and methylation in anthers at HS [90]. In another study, transcriptome analysis was conducted between a susceptible and resistant rice genotype under HS for its impact on panicle development [78].…”

Reading between the Lines: RNA-seq Data Mining Reveals the Alternative Message of the Rice Leaf Transcriptome in Response to Heat Stress

“…Essentially, leaves play an important role in adaptive strategies to overcome heat stress. For example, several studies in rice leaves observed differential expression of transcripts and proteins of dozens of genes upon heat shock [ 19 , 20 , 21 , 22 , 23 ]. Shi et al [ 23 ] also demonstrated that high night temperature treatment did not cause any loss in rice spikelet fertility, whereas it caused a significant decline in overall plant biomass that ultimately resulted in yield and grain quality losses.…”