Transcriptomic and physiological analyses reveal the acquisition of somatic embryogenesis potential in Agapanthus praecox

Yue, Jianhua; Dong, Yan; Du, Changming; Shi, Yabing; Teng, Yun

doi:10.1016/j.scienta.2022.111362

Cited by 9 publications

(6 citation statements)

References 81 publications

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“…To reveal the regulatory mechanism of EC formation, 13,267 DEGs, with 3682 upregulated and 9585 down-regulated (Figures S1 and S2) genes, were further annotated to plant hormone pathways, particularly the auxin, cytokinin, and ABA pathways, by GO and KEGG analysis (Figures S3 and S4), which is consistent with previous research [48,49], indicating the requirement of plant hormones in SE in P. mongolica. Furthermore, there is no genomic data for P. mongolica, which severely hinders research into its molecular biology.…”

Section: Discussionsupporting

confidence: 84%

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Transcriptome Analysis of Embryogenic and Non-Embryogenic Callus of Picea Mongolica

Wang

Bao

et al. 2023

CIMB

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Picea mongolica is a rare tree species in China, which is of great significance in combating desertification and improving the harsh ecological environment. Due to the low rate of natural regeneration, high mortality, and susceptibility to pests and cold springs, Picea mongolica has gradually become extinct. At present, somatic embryogenesis (SE) is the most effective method of micro-proliferation in conifers, but the induction rate of embryogenic callus (EC) is low, and EC is difficult to differentiate from non-embryonic callus (NEC). Therefore, the EC and NEC of Picea mongolica were compared from the morphology, histological, physiological, and transcriptional levels, respectively. Morphological observation showed that the EC was white and transparent filamentous, while the NEC was compact and brownish-brown lumpy. Histological analyses showed that the NEC cells were large and loosely arranged; the nuclei attached to the edge of the cells were small; the cytoplasm was low; and the cell gap was large and irregular. In the EC, small cells, closely arranged cells, and a large nucleus and nucleolus were observed. Physiological studies showed significant differences in ROS-scavenging enzymes between the EC and NEC. Transcriptome profiling revealed that 13,267 differentially expressed genes (DEGs) were identified, 3682 were up-regulated, and 9585 were down-regulated. In total, 63 GO terms had significant enrichment, 32 DEGs in plant hormone signal transduction pathway were identified, and 502 different transcription factors (TFs) were characterized into 38 TF families. Meanwhile, we identified significant gene expression trends associated with somatic embryo development in plant hormones (AUX/IAA, YUCCA, LEA, etc.), stress (GST, HSP, GLP, etc.), phenylpropanoid metabolism (4CL, HCT, PAL, etc.), and transcription factors (AP2/ERF, MYB, WOX, etc.). In addition, nine genes were chosen for RT-qPCR, and the results were consistent with RNA-Seq data. This study revealed the changes in morphology, histology, physiology, and gene expression in the differentiation of NEC into EC and laid the foundation for finding the key genes to promote EC formation.

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Section: Discussionsupporting

confidence: 84%

“…Auxin plays a vital role in regulating cell division, differentiation, and the establishment of embryonic polarity [49,50]. YUC1, YUC4, YUC10, and YUC11, which are members of the YUCCAs family that encode for important enzymes in the Trp-dependent auxin production pathway, are expressed in discrete groups of cells during embryogenesis [23].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis of Embryogenic and Non-Embryogenic Callus of Picea Mongolica

Wang

Bao

et al. 2023

CIMB

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“…In vitro assays demonstrated that maltose can protect proteins, membranes, and the photosynthetic electron transport chain at physiologically relevant concentrations under abiotic stress ( Kaplan and Guy, 2004 ). In addition, sucrose and maltose are important energy carriers and can be hydrolysed into glucose for energy production ( Krasensky and Jonak, 2012 ; Yue et al., 2021 ). According to the KEGG metabolic pathway analysis, galactinol and α-D-galactose can also be directly involved with or converted into D-glucose to participate in glycolysis and the TCA cycle.…”

Section: Discussionmentioning

confidence: 99%

Exogenous leucine alleviates heat stress and improves saponin synthesis in Panax notoginseng by improving antioxidant capacity and maintaining metabolic homeostasis

Liu

Yang

et al. 2023

Front. Plant Sci.

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Panax notoginseng saponins (PNSs) are used as industrial raw materials to produce many drugs to treat cardio-cerebrovascular diseases. However, it is a heat-sensitive plant, and its large-scale artificial cultivation is impeded by high temperature stress, leading to decreases in productivity and PNSs yield. Here, we examined exogenous foliar leucine to alleviate heat stress and explored the underlying mechanism using metabolomics. The results indicated that 3 and 5 mM exogenous foliar leucine significantly alleviated heat stress in one-year- and two-year-old P. notoginseng in pots and field trials. Exogenous foliar leucine enhanced the antioxidant capacity by increasing the activities of antioxidant enzymes (POD, SOD) and the contents of antioxidant metabolites (amino acids). Moreover, exogenous foliar leucine enhanced carbohydrate metabolism, including sugars (sucrose, maltose) and TCA cycle metabolites (citric acid, aconitic acid, succinic acid and fumaric acid), in P. notoginseng leaves, stems, and fibrous roots to improve the energy supply of plants and further alleviate heat stress. Field experiments further verified that exogenous foliar leucine increased the productivity and PNSs accumulation in P. notoginseng. These results suggest that leucine application is beneficial for improving the growth and quality of P. notoginseng under heat stress. It is therefore possible to develop plant growth regulators based on leucine to improve the heat resistance of P. notoginseng and other crops.

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“…As a result, SE requires steady supply and utilization of nitrogen for transcription and translation of pertinent genes, as well as biosynthesis of phytohormones, that cooperatively and synergistically operate during the process (Yue et al 2022). However, the excess consumption of nitrogen (nitrate) from the EC culture medium accounts for low NRA and concurrent high GSA during the in vitro progress of SE.…”

Section: Discussionmentioning

confidence: 99%

Biochemical changes in embryogenic and non-embryogenic callus of Bambusa nutans Wall. during somatic embryogenesis

Rajput

Rathore

Ansari

et al. 2023

Plant Cell Tiss Organ Cult

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Bambusa nutans Wall. is a clump-forming, evergreen bamboo species that is most often found in Southeast Asian forests. Comparative activities of nitrate reductase (NR), glutamine synthetase (GS) and peroxidase (POX) as well as expression of peroxidase isozymes during somatic embryogenesis (SE) were investigated in the segregated embryogenic callus (EC) and non-embryogenic callus (NEC) from the same genotype (CPC-648) of B. nutans Wall. The EC was compact, with large prominent nuclei and dense cytoplasm, while the NEC was fragile, with rudimentary nuclei and hyaline cytoplasm. SE in EC encompassed induction, maturation and germination stages each of 30 days on MS medium supplemented with ascorbic acid 50mg/l, citric acid 25mg/l, cysteine 25mg/l and glutamine 100mg/l + 2, 4-D-2 mg/l + coconut water 10%. EC exhibited dedifferentiation and growth leading to SE, while NEC remained amorphous loose lump throughout. EC had lower NR and POX activities and less number of peroxidase isozymes but higher GS activity than NEC during three stages of SE. NR activity followed a trend as induction > germination > maturation (EC and NEC); GS activity, maturation > germination >induction (EC) and induction >germination >maturation (NEC) and POX activity, maturation > induction > germination (EC) and induction > germination > maturation (NEC). Compared to NEC, EC exhibited less peroxidase isozymes. SE is an epigenetically regulated process that leads to the expression of enzymes involved in primary metabolism in EC and secondary metabolism in NEC. GS activity corresponding to SE process may be developed as biochemical marker. A chaotic scenario, rather from being a marker of SE, is indicated by high levels of peroxidase activity and isozyme expression.

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Transcriptomic and physiological analyses reveal the acquisition of somatic embryogenesis potential in Agapanthus praecox

Cited by 9 publications

References 81 publications

Transcriptome Analysis of Embryogenic and Non-Embryogenic Callus of Picea Mongolica

Transcriptome Analysis of Embryogenic and Non-Embryogenic Callus of Picea Mongolica

Exogenous leucine alleviates heat stress and improves saponin synthesis in Panax notoginseng by improving antioxidant capacity and maintaining metabolic homeostasis

Biochemical changes in embryogenic and non-embryogenic callus of Bambusa nutans Wall. during somatic embryogenesis

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