Transcriptomic and cellular response to bacterial challenge (pathogenic Vibrio parahaemolyticus) in farmed juvenile Haliotis rufescens fed with or without probiotic diet

Silva-Aciares, Fernando; Moraga, Dario; Auffret, Michel; Tanguy, Arnaud; Riquelme, Carlos

doi:10.1016/j.jip.2013.03.004

Cited by 22 publications

(8 citation statements)

References 83 publications

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“…Phagocytosis in marine bivalves is a major immune function that is sensitive to internal and external stressors (Donaghy et al, 2009a;Hégaret et al, 2011;Jauzein et al, 2013;Silva-Aciares et al, 2013). In the present study, the phagocytic capacity of the granulocytes from mussels exposed to high air temperature significantly decreased 102 h after the experiment started.…”

Section: Discussionmentioning

confidence: 42%

Effect of Extreme Heatwaves on the Mortality and Cellular Immune Responses of Purplish Bifurcate Mussel Mytilisepta virgata (Wiegmann, 1837) (=Septifer virgatus) in Indoor Mesocosm Experiments

Hong

Kim

et al. 2021

Front. Mar. Sci.

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In the rocky intertidal environment, the frequency and duration of heatwaves have increased over the last decade, possibly due to global climate change. Heatwaves often result in lethal or sub-lethal disturbances in benthic animals by changing their metabolic activities. In this study, we investigated the impacts of extreme heatwave stress on the hemocyte functions of Mytilisepta virgata and subsequent mortality to gain a better understanding of the potential causes and consequences of mass mortality events in this mussel during summer. We discriminated three types of hemocytes in the hemolymph, granulocytes, hyalinocytes, and blast-like cells, using flow cytometry and revealed that granulocytes were the major hemocyte involved in cellular defensive activities, such as phagocytosis and reactive oxygen species (ROS) production. For the experiment, mussels were exposed to a 40°C air temperature for 12 h per day over 5 days under laboratory conditions as a simulated semi-diurnal tidal cycle. Mortality began to occur within 3 days after beginning the experiment, and all mussels had died by the end of the experiment. Flow cytometry indicated that the mussels exposed to high air temperatures produced significantly more ROS than did the control mussels within 2 days after the onset of the experiment, which may have caused oxidative stress. Such high levels of ROS in the hemolymph increased DNA damage in hemocytes after 3 days of exposure and decreased the phagocytosis of hemocytes 4 days after the experiment began. The observed mortality and decline in immune capacity suggested that an extreme heat event occurring in the rocky intertidal ecosystem during summer could exert sublethal to lethal impacts on macrobenthic animals.

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Section: Discussionmentioning

confidence: 42%

Effect of Extreme Heatwaves on the Mortality and Cellular Immune Responses of Purplish Bifurcate Mussel Mytilisepta virgata (Wiegmann, 1837) (=Septifer virgatus) in Indoor Mesocosm Experiments

Hong

Kim

et al. 2021

Front. Mar. Sci.

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“…V. parahaemolyticus, indeed, is a human bacterial pathogen widely occurring in marine environments, frequently isolated from a variety of seafood, including bivalves, crustaceans, and fish [67]. Vibriosis is currently responsible for most disease outbreaks in aquaculture [31][32][33][34]68].…”

Section: Discussionmentioning

confidence: 99%

“…In highly anthropized marine environments, such as intensive or confined mariculture systems, the excessive release of excreta from farmed species and organic matter from uneaten feed create favorable conditions for pathogenic bacteria growth, especially Vibrio, responsible for diseases and high mortality in target species, with consequent economic losses [31][32][33][34][35][36][37]. To overcome this problem, the use of antibiotics has spread despite the increase in production costs and the negative consequences on farmed species and the surrounding environment.…”

Section: Introductionmentioning

confidence: 99%

Filtering Activity and Nutrient Release by the Keratose Sponge Sarcotragus spinosulus Schmidt, 1862 (Porifera, Demospongiae) at the Laboratory Scale

Trani

Corriero

Pinto

et al. 2021

JMSE

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Sponges are an important constituent of filter-feeder benthic communities, characterized by high ecological plasticity and abundance. Free bacteria constitute an important quota of their diet, making them excellent candidates in aquaculture microbial bioremediation, where bacteria can be a serious problem. Although there are studies on this topic, certain promising species are still under investigation. Here we report applied microbiological research on the filtering activity of Sarcotragus spinosulus on two different concentrations of the pathogenic bacterium Vibrio parahaemolyticus in a laboratory experiment. To evaluate the effects of the filtration on the surrounding nutrient load, the release of ammonium, nitrate, and phosphate was also measured. The results obtained showed the efficient filtration capability of S. spinosulus as able to reduce the Vibrio load with a maximum retention efficiency of 99.72% and 99.35% at higher and lower Vibrio concentrations, respectively, and remarkable values of clearance rates (average maximum value 45.0 ± 4.1 mL h−1 g DW−1) at the highest Vibrio concentration tested. The nutrient release measured showed low values for each considered nutrient category at less than 1 mg L−1 for ammonium and phosphate and less than 5 mg L−1 for nitrate. The filtering activity and nutrient release by S. spinosulus suggest that this species represents a promising candidate in microbial bioremediation, showing an efficient capability in removing V. parahaemolyticus from seawater with a contribution to the nutrient load.

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“…También existen estudios realizados en H. rufescens, donde se ha comprobado la actividad antagonista de cepas pertenecientes a los géneros Bacillus y Vibrio, así como a la especie Agarivorans albus (Silva-Aciares et al 2013). Además, demostraron que ejemplares de este gasterópodo alimentados con probióticos y desafiados con V. parahaemolyticus, experimentan cambios metabólicos a nivel transcriptómico, como una menor expresión de genes en respuesta a estrés, y una mejor condición inmune, en comparación cuando no se adicionan probióticos, lo que podría explicar el efecto benéfico de estas bacterias (Silva-Aciares et al 2013).…”

Section: Agentes Probióticos De Uso Potencial En El Cultivo De Moluscosunclassified

Bacteriología asociada al cultivo de moluscos en Chile: Avances y perspectivas

Fuente

Miranda

Faúndez³

2015

Rev. biol. mar. oceanogr.

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Excessive fishing effort has led to overexploitation and depletion of major benthic fisheries in Chile, so it has been encouraged cultivation of major overexploited benthic resources in controlled environments, mainly those which are not possible to collect seeds from environment. In this review, the current knowledge on bacteriology associated with shellfish farming in controlled systems in Chile, including both aspects associated with development of infectious diseases of bacterial aetiology as well as the use of microorganisms to reduce mortality of cultured species, is described. The majority of bacteriological studies in Chilean shellfish culture were done in the Chilean scallop, Argopecten purpuratus and were related to identifying pathogens isolated from mass mortalities occurred in larval and/or juvenile cultures and the verification of their pathogenic activity performing bioassays under controlled condition bioassays. There are also a large number of studies on bacteria exhibiting inhibitory activities against shellfish pathogenic and their protective effects under controlled conditions. Despite the above, still are required more studies to know the structure and dynamics of the microbiota associated to mollusk culture, bacterial pathogens and molecular elements encoding for their pathogenicity.

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Transcriptomic and cellular response to bacterial challenge (pathogenic Vibrio parahaemolyticus) in farmed juvenile Haliotis rufescens fed with or without probiotic diet

Cited by 22 publications

References 83 publications

Effect of Extreme Heatwaves on the Mortality and Cellular Immune Responses of Purplish Bifurcate Mussel Mytilisepta virgata (Wiegmann, 1837) (=Septifer virgatus) in Indoor Mesocosm Experiments

Effect of Extreme Heatwaves on the Mortality and Cellular Immune Responses of Purplish Bifurcate Mussel Mytilisepta virgata (Wiegmann, 1837) (=Septifer virgatus) in Indoor Mesocosm Experiments

Filtering Activity and Nutrient Release by the Keratose Sponge Sarcotragus spinosulus Schmidt, 1862 (Porifera, Demospongiae) at the Laboratory Scale

Bacteriología asociada al cultivo de moluscos en Chile: Avances y perspectivas

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