Transcriptomic analysis of pepper plants provides insights into host responses to Fusarium solani infestation

Lau, Ee Tiing; Khew, Choy Yuen; Hwang, Siaw San

doi:10.1016/j.jbiotec.2020.03.014

Cited by 10 publications

(5 citation statements)

References 55 publications

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“…The 2.3-fold and 1.8-fold upregulation of MDHAR-related genes in T5 and T3 observed in this study suggested that increased years of continuous cropping may lead to the increased susceptibility of sugar beet to the soil environment stress (Tan et al, 2021). In the plant-pathogen interaction pathway, both T3 and T5 had over 20 DEGs enriched compared to T1, which revealed that those DEGs could have responses to pathogens that increased in the soil after years of continuous cropping (Huang W. et al, 2020;Lau et al, 2020).…”

Section: Discussionmentioning

confidence: 55%

Integration of Transcriptomics and Metabolomics Reveals the Responses of Sugar Beet to Continuous Cropping Obstacle

et al. 2021

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Sugar beet is vulnerable to years of continuous cropping, and allelopathy is one of the important factors leading to continuous cropping disorder. To explore the physiological and molecular mechanisms behind continuous cropping obstacles on sugar beet, this study combined transcriptomics and metabolomics to analyze the effects of different years of continuous cropping on metabolite changes, differential gene expression, and root exudate regulation in sugar beet. We collected sugar beet’s root samples from 1–, 3–, and 5-year continuous cropping systems for metabolome and transcriptome analyses. Our data revealed that T3 and T5 had 50 and 33 metabolites significantly different from T1, respectively. The autotoxic substance salicylaldehyde was found to continuously accumulate in root exudates with increasing years of continuous cropping. Sucrose was highly reduced in T3 (4.05-fold decrease) and T5 (2.01-fold decrease) compared to T1. Respectively, 2,660 and 3,515 differentially expressed genes (DEGs) were significantly regulated in T3 and T5 compared to T1. The Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses showed that metabolic pathways and biosynthesis of secondary metabolites were perturbed in T3 and T5 vs. T1. Integrated metabolomics analyses identified 73 DEGs involved in enriched metabolic pathways, all of which were the oxidation-reduction process pathways. In conclusion, this study provides evidence that continuous cropping obstacles can change the metabolome and transcriptome of sugar beet, affecting its growth and quality.

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Section: Discussionmentioning

confidence: 55%

Integration of Transcriptomics and Metabolomics Reveals the Responses of Sugar Beet to Continuous Cropping Obstacle

et al. 2021

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“…The main compounds were γ-elemene (38.33 ± 3.80% and 44.36 ± 4.06%) ( Figure 1 D), curzerene (23.21 ± 4.96% and 23.39 ± 4.45%), and δ-elemene (6.93 ± 0.77% and 13.84 ± 0.80%), respectively. Several Piper species present γ-elemene as one of the main components in their EOs, such as P. gaudichaudianum Kunth (5.4%) [ 41 ], P. marginatum (3.75%) [ 64 ], P. nemorense (6.8%) [ 48 ], and P. vicosanum (14.16%) [ 65 ].…”

Section: Resultsmentioning

confidence: 99%

“…piperis and F. oxysporum are the most notably responsible for the fungal diseases in black pepper that negatively interfere in its fruit production and the plant quality [ 39 , 40 ]. Moreover, they cause significant yield loss due to the severe damage, since they do not present apparent symptoms in the initial phase of infection [ 41 ]. Strategies of control have not been totality efficient in treating and preventing black pepper diseases.…”

Section: Introductionmentioning

confidence: 99%

Secondary Metabolic Profile as a Tool for Distinction and Characterization of Cultivars of Black Pepper (Piper nigrum L.) Cultivated in Pará State, Brazil

Barata

Andrade

Ramos

et al. 2021

IJMS

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This study evaluated the chemical compositions of the leaves and fruits of eight black pepper cultivars cultivated in Pará State (Amazon, Brazil). Hydrodistillation and gas chromatography–mass spectrometry were employed to extract and analyze the volatile compounds, respectively. Sesquiterpene hydrocarbons were predominant (58.5–90.9%) in the cultivars “Cingapura”, “Equador”, “Guajarina”, “Iaçará”, and “Kottanadan”, and “Bragantina”, “Clonada”, and “Uthirankota” displayed oxygenated sesquiterpenoids (50.6–75.0%). The multivariate statistical analysis applied using volatile composition grouped the samples into four groups: γ-Elemene, curzerene, and δ-elemene (“Equador”/“Guajarina”, I); δ-elemene (“Iaçará”/“Kottanadan”/“Cingapura”, II); elemol (“Clonada”/“Uthirankota”, III) and α-muurolol, bicyclogermacrene, and cubebol (“Bragantina”, IV). The major compounds in all fruit samples were monoterpene hydrocarbons such as α-pinene, β-pinene, and limonene. Among the cultivar leaves, phenolics content (44.75–140.53 mg GAE·g−1 FW), the enzymatic activity of phenylalanine-ammonia lyase (20.19–57.22 µU·mL−1), and carotenoids (0.21–2.31 µg·mL−1) displayed significant variations. Due to black pepper’s susceptibility to Fusarium infection, a molecular docking analysis was carried out on Fusarium protein targets using each cultivar’s volatile components. F. oxysporum endoglucanase was identified as the preferential protein target of the compounds. These results can be used to identify chemical markers related to the susceptibility degree of black pepper cultivars to plant diseases prevalent in Pará State.

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“…RNA-seq was first used in Black pepper to generate the root transcriptome data set ( Gordo et al, 2012 ), followed by studies on leaf ( Joy et al, 2013 ), fruit ( Hu et al, 2015 ), and phenylpropanoid metabolism in response to the foot rot disease pathogen ( Hao et al, 2016 ). A comparative transcriptome of three different black pepper cultivars for exploring the genetic regulation of flower and fruit development ( Khew et al, 2020 ), a leaf transcriptome in response to Fusarium solani infestation ( Lau et al, 2020 ) for drought tolerance ( Negi et al, 2021 ) are also reported. A novel natural cyclobutane-containing alkaloid, piperine from Piper species has also been reviewed ( Vasavirama and Upender, 2014 ).…”

Section: Transcriptomic Studies: Comprehensive and Quantitative Resou...mentioning

confidence: 99%

A comprehensive review on genomic resources in medicinally and industrially important major spices for future breeding programs: Status, utility and challenges

Das,

Chandra,

Negi

et al. 2023

Current Research in Food Science

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Transcriptomic analysis of pepper plants provides insights into host responses to Fusarium solani infestation

Cited by 10 publications

References 55 publications

Integration of Transcriptomics and Metabolomics Reveals the Responses of Sugar Beet to Continuous Cropping Obstacle

Integration of Transcriptomics and Metabolomics Reveals the Responses of Sugar Beet to Continuous Cropping Obstacle

Secondary Metabolic Profile as a Tool for Distinction and Characterization of Cultivars of Black Pepper (Piper nigrum L.) Cultivated in Pará State, Brazil

A comprehensive review on genomic resources in medicinally and industrially important major spices for future breeding programs: Status, utility and challenges

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