Transcriptomic analysis of melon/squash graft junction reveals molecular mechanisms potentially underlying the graft union development

Xu, Chuanqiang; Zhang, Ying; Zhao, Mingzhe; Liu, Yiling; Xu, Xin; Li, Tianlai

doi:10.7717/peerj.12569

Cited by 13 publications

(10 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It has also been reported that grafting induces differences in the transcriptome profile of grafted parents in plants, such as grapevines 39 , 40 . However, much attention had been absorbed on the connection of the graft union.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome and physiological analyses reveal new insights into delayed incompatibility formed by interspecific grafting

Wang

Zhao

et al. 2023

Sci Rep

View full text Add to dashboard Cite

Pinus elliottii used as rootstock instead of homologous rootstock, have been proved to accelerate early growth of the scion (Pinus massoniana), for cultivation of large diameter wood. However, the basal diameter of scions in heterologous grafts was significantly smaller than self-graft 10 years later, according to field investigation, which was opposed to cultivation objectives. Although advantage of heterologous grafts has been reported, less is known about the long term effect of heterologous rootstock on scions of P. massoniana. The aim of present study was to investigate the mechanism of the above difference. Toward this aim, the growth traits and physiological characteristics of scions in the two graft groups were studied, and the underlying mechanism was preliminarily explored through transcriptome sequencing technology. Results showed that scions of heterologous grafts had less TSCA compared to self-grafts, while no significant difference of plant height, number of branches and canopy volume between two graft groups. Besides, scion leaves of heterologous grafts displayed higher antioxidant enzyme activity and lower chlorophyll content. And interactions between rootstocks and scions had also changed the mineral element composition of scion leaves. Compared with homologous grafts, scion leaves of heterologous grafts accumulated more K+, Mg2+ and Zn2+, but less Ca2+,which have been proved to be conducive to the growth of stem diameter of P. massoniana. Moreover, a comparative transcriptome analysis of two graft groups showed that DEGs between them were mainly caused by the specificity of rootstock. GO and KEGG analysis found that heterologous rootstock had different gene expression preferences, and the gene expression level between rootstocks and scions were significantly different, such as auxin auxin-related genes and stress responsive genes. That may imply that auxin pathway played an important role not only in grafting healing process, but also in maintaining the growth between scion and stock. Summary of all above results, we concluded that the long term effect of heterologous rootstock on scions may be unsatisfactory with the later rapidly growth of scion, probably due to delayed graft incompatibility between scion and stock of heterologous grafts. This study may remind us that the long-term growth of the scion deserves attention as well as the healing process, which could also provide a basis for delayed graft incompatibility.

show abstract

Section: Discussionmentioning

confidence: 99%

Transcriptome and physiological analyses reveal new insights into delayed incompatibility formed by interspecific grafting

Wang

Zhao

et al. 2023

Sci Rep

View full text Add to dashboard Cite

show abstract

“…RNA-seq technology has been used to explore the potential transcriptional regulatory mechanisms of the graft healing process ( Cheong et al., 2002 ; Cookson et al, 2013 ; Liu et al, 2015 ; Chen et al, 2017 ; Melnyk et al, 2018 ; Mo et al, 2018 ; Xu et al, 2021 ). In order to further analyze the potential molecular networks of exogenous NAA regulating graft healing of oriental melon scion grafted onto squash rootstock, we performed the transcriptome analysis of graft junction at the IL, CA, and VB stage by CK vs. NAA using Melon genome and Cucurbita moschata (Rifu) genome, respectively, and deeply excavated the DEGs involved in hormone signal-transduction, ROS scavenging, and vascular bundle formation during graft union development ( Figs.…”

Section: Discussionmentioning

confidence: 99%

“…Total RNA extraction, library construction, and RNA-Seq were performed by Biomarker Technology Co. (Beijing, China, https://www.biocloud.net/ ). RNA concentration was measured using NanoDrop 2000 (Thermo Fisher Scientific, Waltham, MA, USA), and the detailed protocol was previously described ( Xu et al, 2021 ). The raw sequencing data had been uploaded to the NCBI Sequence Read Archive (SRA) with the accession number PRJNA655799 and PRJNA689873 .…”

Section: Methodsmentioning

confidence: 99%

Transcriptomic analysis and physiological characteristics of exogenous naphthylacetic acid application to regulate the healing process of oriental melon grafted onto squash

Guo

et al. 2022

PeerJ

Self Cite

View full text Add to dashboard Cite

The plant graft healing process is an intricate development influenced by numerous endogenous and environmental factors. This process involves the histological changes, physiological and biochemical reactions, signal transduction, and hormone exchanges in the grafting junction. Studies have shown that applying exogenous plant growth regulators can effectively promote the graft healing process and improve the quality of grafted plantlets. However, the physiological and molecular mechanism of graft healing formation remains unclear. In our present study, transcriptome changes in the melon and cucurbita genomes were analyzed between control and NAA treatment, and we provided the first view of complex networks to regulate graft healing under exogenous NAA application. The results showed that the exogenous NAA application could accelerate the graft healing process of oriental melon scion grafted onto squash rootstock through histological observation, increase the SOD, POD, PAL, and PPO activities during graft union development and enhance the contents of IAA, GA 3 , and ZR except for the IL stage. The DEGs were identified in the plant hormone signal-transduction, phenylpropanoid biosynthesis, and phenylalanine metabolism through transcriptome analysis of CK vs. NAA at the IL, CA, and VB stage by KEGG pathway enrichment analysis. Moreover, the exogenous NAA application significantly promoted the expression of genes involved in the hormone signal-transduction pathway, ROS scavenging system, and vascular bundle formation.

show abstract

“…Transcriptomic analysis revealed the differential expression of 112 candidate unigenes involved in auxin and cytokinin signaling pathways in a study involving pecan graft healing (Qiu et al., 2016). Furthermore, transcriptomic analysis of the melon/pumpkin graft junction revealed that genes involved in hormone signaling and lignin biosynthesis play critical roles in graft healing (Xu et al., 2021). These studies suggest that transcriptome profiling may be used to study the molecular mechanisms involved in the engraftment process.…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptome analysis of conventionally cultivated tobacco and tobacco grafted to eggplant

2023

View full text Add to dashboard Cite

Tobacco (Nicotiana tabacum L.) grafted to eggplant (Solanum melongena L.) has been shown to limit nicotine content, however, the molecular mechanism of this graft combination remains unclear. In this study, we performed a genome‐wide transcriptional analysis of Zhongyan‐100 leaves, that were conventionally planted and grafted onto eggplant rootstocks, using high‐throughput sequencing. The nicotine content in the leaves of the tobacco/eggplant grafting group was significantly decreased compared to the tobacco group. A total of 2389 differentially expressed genes (DEGs) were identified between the tobacco/eggplant group and tobacco group. Functional annotation revealed that many DEGs were involved in glutathione and linoleic acid metabolism, and phenylpropanoid, sesquiterpenoid, and triterpenoid biosynthesis. To explore whether nicotine level in tobacco changes after grafting, the expression of PMT, QPT, A622, and BBLa in the roots and stems was elucidated using quantitative PCR. This study found that the expression of these genes in the roots of the tobacco/eggplant group was significantly lower than that in the tobacco group. In addition, the expression of PMT, A622, and BBLa in tobacco stems of the tobacco/eggplant group was significantly higher than those in tobacco stems of the tobacco group and leaves of the tobacco/eggplant group. Our comprehensive analysis provides basic information on the genes and metabolic signaling pathways involved in Zhongyan‐100/eggplant grafting. Additionally, this study found that the expression of nicotine‐related genes changed during the grafting process; however, the specific molecular mechanisms underpinning this effect requires further exploration.

show abstract

Transcriptomic analysis of melon/squash graft junction reveals molecular mechanisms potentially underlying the graft union development

Cited by 13 publications

References 57 publications

Transcriptome and physiological analyses reveal new insights into delayed incompatibility formed by interspecific grafting

Transcriptome and physiological analyses reveal new insights into delayed incompatibility formed by interspecific grafting

Transcriptomic analysis and physiological characteristics of exogenous naphthylacetic acid application to regulate the healing process of oriental melon grafted onto squash

Comparative transcriptome analysis of conventionally cultivated tobacco and tobacco grafted to eggplant

Contact Info

Product

Resources

About