Transcriptomic analysis and physiological characteristics of exogenous naphthylacetic acid application to regulate the healing process of oriental melon grafted onto squash

Xu, Chuanqiang; Wu, Fang; Guo, Jieying; Hou, Shuan; Wu, Xiaofang; Xin, Ying

doi:10.7717/peerj.13980

Cited by 3 publications

(1 citation statement)

References 79 publications

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“…The plant hormones IAA, CTK, and GAs, were closely related to callus formation, vascular bundle development, and reconnection during the graft union healing process [41][42][43]. Some reports showed that applying exogenous naphthylacetic acid (NAA) could significantly promote graft union formation [44]. It has been reported that several essential genes play a role in regulating graft union healing.…”

Section: Discussionmentioning

confidence: 99%

Exogenous Melatonin Application Accelerated the Healing Process of Oriental Melon Grafted onto Squash by Promoting Lignin Accumulation

Zhu,

Guo,

et al. 2024

IJMS

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Melatonin (MT) is a vital hormone factor in plant growth and development, yet its potential to influence the graft union healing process has not been reported. In this study, we examined the effects of MT on the healing of oriental melon scion grafted onto squash rootstock. The studies indicate that the exogenous MT treatment promotes the lignin content of oriental melon and squash stems by increasing the enzyme activities of hydroxycinnamoyl CoA ligase (HCT), hydroxy cinnamaldehyde dehydrogenase (HCALDH), caffeic acid/5-hydroxy-conifer aldehyde O-methyltransferase (COMT), caffeoyl-CoA O-methyltransferase (CCoAOMT), phenylalanine ammonia-lyase (PAL), 4-hydroxycinnamate CoA ligase (4CL), and cinnamyl alcohol dehydrogenase (CAD). Using the oriental melon and squash treated with the exogenous MT to graft, the connection of oriental melon scion and squash rootstock was more efficient and faster due to higher expression of wound-induced dedifferentiation 1 (WIND1), cyclin-dependent kinase (CDKB1;2), target of monopteros 6 (TMO6), and vascular-related NAC-domain 7 (VND7). Further research found that the exogenous MT increased the lignin content of the oriental melon scion stem by regulating CmCAD1 expression, and then accelerated the graft healing process. In addition, the root growth of grafted seedlings treated with the exogenous MT was more vigorous.

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Section: Discussionmentioning

confidence: 99%

Exogenous Melatonin Application Accelerated the Healing Process of Oriental Melon Grafted onto Squash by Promoting Lignin Accumulation

Zhu,

Guo,

et al. 2024

IJMS

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IAA-miR164a-NAC100L1 module mediates symbiotic incompatibility of cucumber/pumpkin grafted seedlings through regulating callose deposition

Yuan,

Jin,

et al. 2023

Horticulture Research

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Grafting is one of the key technologies to overcome the obstacles of continuous cropping, and improve crop yield and quality. However, the symbiotic incompatibility between rootstock and scion affects the normal growth and development of grafted seedlings after survival. The specific molecular regulation mechanism of graft incompatibility is still largely unclear. In this study, we found that IAA-miR164a-NAC100L1 module induced callose deposition to mediate the symbiotic incompatibility of cucumber/pumpkin grafted seedlings. The incompatible combination (IG) grafting interface accumulated more callose, and the activity of callose synthase (CmCalS1) and IAA content were significantly higher than those in compatible combination (CG). Treatment with IAA polar transport inhibitor in the root of the IG plants decreased CmCalS activity and callose content. Furthermore, IAA negatively regulated the expression of Cm-miR164a, which directly targeted cleavage of CmNAC100L1. Interestingly, CmNAC100L1 interacted with CmCalS1 to regulate its activity. Further analysis showed that the interaction between CmNAC100L1 and CmCalS1 in the IG plants enhanced, but decreased the activity of CmCalS1 in the CG plants. Point mutation analysis revealed that threonine at the 57th position of CmCalS1 protein played a critical role to maintain its enzyme activity in the incompatible rootstock. Thus, IAA inhibited the expression of Cm-miR164a to elevate the expression of CmNAC100L1, which promoted CmNAC100L1 interaction with CmCalS1 to enhance CmCalS1 activity, resulting callose deposition and symbiotic incompatibility of cucumber/pumpkin grafted seedlings.

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An Experimental Study on the Effect of Cutting Angle on the Growth of Grafted Watermelon Seedlings Using the One-Cotyledon Grafting Method

et al. 2023

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The labor-efficient automation of grafting has been recognized as a key factor in the wider adoption of grafting. In growing cucurbits, the root pruned one-cotyledon grafting method is the most commonly used method with grafting machines. The cutting angle, which affects the matching of the rootstock and scion, is key to the survival of the graft seedling. In the production of cucurbit graft seedlings, the cutting parameters are established based on experience, leading to low grafting success. To determine accurate cutting parameters, the watermelon cv. ‘Zaojia84-24′ was used as the scion and the pumpkin cv. ‘Zhenzhuang’ was used as rootstock, and two one-way experiments investigating the cutting angle of the watermelon scion and the rootstock as factors were conducted. The cutting angle of the rootstock and scion had no significant effect on the xylem reconnection rate or the grafting survival rate. A larger cutting angle for the rootstock and scion led to a delay in the reconnection of the phloem. Different cutting angles for the scion significantly affected the growth of the scion after grafting. Compared with a scion graft cutting angle of 10° (SL), graft cutting angles of 14° (SM) and 20° (SS) led to significantly greater scion dry weights, with increases of 16.00% and 18.61%, respectively. Different cutting angles of the rootstock significantly affected the growth of roots after grafting. Compared with a rootstock graft cutting angle of 10° (RL), graft cutting angles of 17° (RM) and 27° (RS) led to significantly greater root dry weights, with increases of 29.33% and 22.54%, respectively. The results of this study can provide a reference for the design of cutting mechanisms for cucurbit grafting robots, improving the cutting precision of grafting robots.

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Transcriptomic analysis and physiological characteristics of exogenous naphthylacetic acid application to regulate the healing process of oriental melon grafted onto squash

Cited by 3 publications

References 79 publications

Exogenous Melatonin Application Accelerated the Healing Process of Oriental Melon Grafted onto Squash by Promoting Lignin Accumulation

Exogenous Melatonin Application Accelerated the Healing Process of Oriental Melon Grafted onto Squash by Promoting Lignin Accumulation

IAA-miR164a-NAC100L1 module mediates symbiotic incompatibility of cucumber/pumpkin grafted seedlings through regulating callose deposition

An Experimental Study on the Effect of Cutting Angle on the Growth of Grafted Watermelon Seedlings Using the One-Cotyledon Grafting Method

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