Transcriptomes of bovine ovarian follicular and luteal cells

Romereim, Sarah M.; Summers, Adam F.; Pohlmeier, William E.; Zhang, Pan; Hou, Xiaoying; Talbott, Heather; Cushman, Robert A.; Wood, Jennifer R.; Davis, John S.; Cupp, Andrea S.

doi:10.1016/j.dib.2016.11.093

Cited by 12 publications

(36 citation statements)

References 6 publications

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“…Using cell size, the LLC purity was ≥80% (contaminating cells may include clumps of endothelial cells or SLC and similarly sized fibroblasts or immune cells) and the SLC purity was ≥95% (contaminating cells may include clumps of endothelial cells or similarly sized fibroblasts or immune cells. Affymetrix RNA microarray analysis generated the transcriptome data (Romereim et al, 2016) [NCBI GEO GSE83524]. An overall low standard deviation profile indicated quality amplification, with slightly higher standard deviations for lower-intensity RNAs suggesting more variability for low copy-number targets [see Fig.…”

Section: Resultsmentioning

confidence: 99%

Gene expression profiling of bovine ovarian follicular and luteal cells provides insight into cellular identities and functions

Romereim¹,

Summers²,

Pohlmeier³

et al. 2017

Molecular and Cellular Endocrinology

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After ovulation, somatic cells of the ovarian follicle (theca and granulosa cells) become the small and large luteal cells of the corpus luteum. Aside from known cell type-specific receptors and steroidogenic enzymes, little is known about the differences in the gene expression profiles of these four cell types. Analysis of the RNA present in each bovine cell type using Affymetrix microarrays yielded new cell-specific genetic markers, functional insight into the behavior of each cell type via Gene Ontology Annotations and Ingenuity Pathway Analysis, and evidence of small and large luteal cell lineages using Principle Component Analysis. Enriched expression of select genes for each cell type was validated by qPCR. This expression analysis offers insight into cell-specific behaviors and the differentiation process that transforms somatic follicular cells into luteal cells.

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Section: Resultsmentioning

confidence: 99%

Gene expression profiling of bovine ovarian follicular and luteal cells provides insight into cellular identities and functions

Romereim¹,

Summers²,

Pohlmeier³

et al. 2017

Molecular and Cellular Endocrinology

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“…It was reported that CYP19A1 and PTGFR could be considered as cellular markers of GCs and luteal cells, respectively. [ 29 ] During the luteal steroidogenesis, StAR delivers cholesterol to the mitochondrial intima, and then P450scc catalyzes pregnenolone synthesis and HSD3B catalyzes progesterone synthesis. Androstenedione was the substrate of estrogen, and CYP19A1 catalyzes estrogen synthesis in the mural and cumulus GCs.…”

Section: Discussionmentioning

confidence: 99%

Quercetin alleviated H₂O₂‐induced apoptosis and steroidogenic impairment in goat luteinized granulosa cells

Yang

et al. 2020

J Biochem & Molecular Tox

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Quercetin (Que) is a natural flavonoid in most plants. Luteinized granulosa cell (LGC) culture is necessary for the study of follicle growth/differentiation. In the present study, we analyzed the role of Que in steroid production and apoptosis in hydrogen peroxide (H2O2)‐treated goat LGCs. The results showed that treatment with H2O2 induced apoptosis in goat LGCs, and treatment with Que decreased LGC apoptosis induced by H2O2 (P < .05), accompanied with the different expressions of BAX, BCL‐2, Caspase 3, and Cleaved caspase 3. Meanwhile, the messenger RNA expressions of nuclear factor erythroid 2 like 2 (Nrf2) and its downstream genes were upregulated with H2O2 +Que treatment, accompanied by the increased cellular viability (P < .05). Furthermore, Que alleviated H2O2‐induced reduction in the secretion of progesterone (P4) (P < .05); however, it had no effect on the secretion of estrogen (E2). Simultaneously, the expressions of StAR and P450scc were increased when treated with Que +H2O2, compared with the group treated with only H2O2 (P < .05). In conclusion, it is observed that Que could alleviate the H2O2‐induced apoptosis and steroidogenic impairment in goat LGCs, which might be mediated by the Nrf2 pathway.

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“…The components of hippo signaling in granulosa cells have been reported in both the mouse [18], hen [27], and human [28]. Moreover, microarray studies of bovine granulosa cells have revealed the transcripts for hippo signaling [29, 30]. Here, we examined YAP and TAZ protein expression in bovine ovaries.…”

Section: Discussionmentioning

confidence: 99%

Yes-associated protein (YAP) is required in maintaining normal ovarian follicle development and function

Plewes

Hou

Zhang

et al. 2018

Preprint

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Yes-associated protein (YAP) is one of the major components of the Hippo signaling pathway, also known as the Salvador/Warts/Hippo (SWH) pathway. Although the exact extracellular signal that controls the Hippo pathway is currently unknown, increasing evidence supports a critical role of the Hippo pathway in embryonic development, regulation of organ size, and carcinogenesis. The ovary is one of few adult tissues that exhibit cyclical changes. Ovarian follicles, the basic units of ovary, are composed of a single oocyte surrounded by expanding layers of granulosa and theca cells. Granulosa cells (GCs) produce sex steroids and growth factors, which facilitate the development of the follicle and maturation of the oocyte. It has been reported that YAP is highly expressed in human GC tumors, but the role of YAP in normal ovarian follicle development is largely unknown. In current study, we examined YAP expression in bovine ovaries. We demonstrate that downstream hippo signaling effector protein, YAP and transcription co-activator, TAZ, are present and localization of both YAP and TAZ are density-dependent. Likewise, YAP and TAZ are critically involved in granulosa cell proliferation. Furthermore, reducing YAP in granulosa cells inhibits FSH-induced aromatase expression and estradiol biosynthesis. The data suggest that YAP plays an important role in the development of ovarian follicles and estradiol synthesis, which are necessary for maintaining normal ovarian function.

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Transcriptomes of bovine ovarian follicular and luteal cells

Cited by 12 publications

References 6 publications

Gene expression profiling of bovine ovarian follicular and luteal cells provides insight into cellular identities and functions

Gene expression profiling of bovine ovarian follicular and luteal cells provides insight into cellular identities and functions

Quercetin alleviated H₂O₂‐induced apoptosis and steroidogenic impairment in goat luteinized granulosa cells

Yes-associated protein (YAP) is required in maintaining normal ovarian follicle development and function

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Transcriptomes of bovine ovarian follicular and luteal cells

Cited by 12 publications

References 6 publications

Gene expression profiling of bovine ovarian follicular and luteal cells provides insight into cellular identities and functions

Gene expression profiling of bovine ovarian follicular and luteal cells provides insight into cellular identities and functions

Quercetin alleviated H2O2‐induced apoptosis and steroidogenic impairment in goat luteinized granulosa cells

Yes-associated protein (YAP) is required in maintaining normal ovarian follicle development and function

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Product

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Quercetin alleviated H₂O₂‐induced apoptosis and steroidogenic impairment in goat luteinized granulosa cells