Transcriptome Sequencing Reveals the Potential Mechanisms of Modified Electroconvulsive Therapy in Schizophrenia

Peng, Wanhong; Tan, Qingyu; Yu, Minglan; Wang, Ping; Wang, Tingting; Yuan, Jixiang; Li, Dongmei; Chen, Dechao; Huang, Chaohua; Tan, Youguo; Liu, Kezhi; Xiang, Bo; Liang, Xuemei

doi:10.30773/pi.2020.0410

Cited by 4 publications

(4 citation statements)

References 36 publications

(51 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We subsequently confirmed this finding by comparing the mRNA expression in different brain regions in patients with SCZ and healthy controls using a public database, and found that EP300 mRNA expression increased in patients with SCZ. 10 These findings seem to indicate that EP300 plays an important role in the improvement of psychiatric symptoms in ECT.…”

Section: Introductionmentioning

confidence: 86%

“…A mutation burden test 9 found that a different mutation load spectrum in the exon region of the EP300 was associated with SCZ. Our preliminary study 10 identified EP300 as a core gene through analyzing the transcriptome sequencing data in 8 schizophrenic patients (BECT and AECT) and 8 healthy controls. We subsequently confirmed this finding by comparing the mRNA expression in different brain regions in patients with SCZ and healthy controls using a public database, and found that EP300 mRNA expression increased in patients with SCZ.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Electroconvulsive Therapy Reduces Protein Expression Level of EP300 and Improves Psychiatric Symptoms and Disturbance of Thought in Patients with Schizophrenia

Peng¹,

Wang²,

Yu³

et al. 2023

NDT

Self Cite

View full text Add to dashboard Cite

Objective Although electroconvulsive therapy (ECT) has been employed as an effective treatment strategy and to improve mental symptoms in schizophrenia (SCZ), its action mechanisms remain unclear. Our previous study found that some genes and biological pathways were closely related to ECT through genetic technology analysis, such as LTP pathway and EP300 . This study combined with healthy controls and symptomatology analysis to further explore the changes of expression of EP300 protein in treatment and related symptoms of SCZ. Methods One hundred and one patients with SCZ and 45 healthy controls (HCs) were enrolled in this study. Patients with SCZ received acute courses of 6 times bilateral ECT. The peripheral blood of patients with SCZ (BECT: before ECT; AECT: after ECT) and the HCs was collected to calculate the changes of expression level of EP300 protein by enzyme-linked immunosorbent assay. The Positive and Negative Symptoms Scale (PANSS) was used to evaluate the severity of symptoms of SCZ patients and the efficiency of the ECT. Results There was a statistical difference of EP300 protein expression in patients with SCZ (BECT and AECT) (F = 114.5, p < 0.05). ECT reduced plasma expression level of EP300 protein in patients with SCZ, which was not statistically different from that in HCs (t = 4.47, p = 0.20). The change of the expression level of EP300 protein in patients with SCZ (BECT and AECT) has a positive correlation with reduction rate of positive symptoms (r = 0.228, p < 0.05) and disturbance of thought (r = 0.219, p < 0.05). Conclusion Our study suggests that the expression level of EP300 protein has a significant change in patients with SCZ treating with ECT, and EP300 may have some connections with positive symptoms and disturbance thought of patients with SCZ.

show abstract

Section: Introductionmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Electroconvulsive Therapy Reduces Protein Expression Level of EP300 and Improves Psychiatric Symptoms and Disturbance of Thought in Patients with Schizophrenia

Peng¹,

Wang²,

Yu³

et al. 2023

NDT

Self Cite

View full text Add to dashboard Cite

show abstract

“…A diferencia de estudios basados en el cambio de un número limitado de genes y/o proteínas, la secuenciación de transcriptomas permite determinar el conjunto de genes cuya transcripción es inducida por la TEC. Usando este tipo de análisis, Peng et al 12 determinaron que la vía de potenciación a largo plazo y el gen EP300 (histona acetiltransferasa), se activan a consecuencia de la TEC en PE (n = 8).…”

Section: Genesunclassified

“…Gen EP300 12 Moléculas Neurotransmisión Niveles de GABA en corteza prefrontal medial 16 Factores neurotróficos Niveles de BDNF 20…”

Section: Genesunclassified

Posibles mecanismos de acción de la terapia electroconvulsiva en esquizofrenia: revisión de la evidencia disponible en investigación con seres humanos

Arancibia

Vargas²,

Abarca

et al. 2022

Rev. méd. Chile

View full text Add to dashboard Cite

A review about the putative mechanisms of action of electroconvulsive therapy in schizophrenia in human researchElectroconvulsive therapy (ECT) has multiple uses in psychiatry, but its mechanisms of action (MA) in patients with schizophrenia (PS) are poorly understood. We synthesize and discuss the available evidence in this regard. We conducted a search for primary human studies and systematic reviews searching MA of ECT in PS published in PubMed/Medline, SciELO, PsycInfo, and the Cochrane Library, including 24 articles. Genetic findings are scarce and inconsistent. At the molecular level, the dopaminergic and GABAergic role stands out. The increase in brain derived neurotrophic factor (BDNF) after ECT, is a predictor of positive clinical outcomes, while the change in N-acetyl aspartate levels would demonstrate a neuroprotective role for ECT. This intervention would improve inflammatory and oxidative parameters, thereby resulting in a symptomatic improvement. ECT is associated with an increase in functional connectivity in the thalamus, right putamen, prefrontal cortex and left precuneus, structures that play a role in the neural default mode network. A decrease in connectivity between the thalamus and the sensory cortex and an enhanced functional connectivity of the right thalamus to right putamen along with a clinical improvement have been reported after ECT. Moreover a volumetric increase in hippocampus and insula has been reported after ECT. These changes could be associated with the biochemical pathophysiology of schizophrenia. Most of the included studies are observational or quasi-experimental, with small sample sizes. However, they show simultaneous changes at different neurobiological levels, with a pathophysiological and clinical correlation. We propose that the research on ECT should be carried out from neurobiological dimensions, but with a clinical perspective.

show abstract

Behavioral and transcriptional effects of repeated electroconvulsive seizures in the neonatal MK-801-treated rat model of schizophrenia

Lee,

Huh,

Park

et al. 2023

Psychopharmacology

View full text Add to dashboard Cite

Transcriptome Sequencing Reveals the Potential Mechanisms of Modified Electroconvulsive Therapy in Schizophrenia

Cited by 4 publications

References 36 publications

Electroconvulsive Therapy Reduces Protein Expression Level of EP300 and Improves Psychiatric Symptoms and Disturbance of Thought in Patients with Schizophrenia

Electroconvulsive Therapy Reduces Protein Expression Level of EP300 and Improves Psychiatric Symptoms and Disturbance of Thought in Patients with Schizophrenia

Posibles mecanismos de acción de la terapia electroconvulsiva en esquizofrenia: revisión de la evidencia disponible en investigación con seres humanos

Behavioral and transcriptional effects of repeated electroconvulsive seizures in the neonatal MK-801-treated rat model of schizophrenia

Contact Info

Product

Resources

About