Transcriptome response of Acetobacter pasteurianus Ab3 to high acetic acid stress during vinegar production

Xia, Kai; Han, Chengcheng; Xu, Jun; Liang, Xinmiao

doi:10.1007/s00253-020-10995-0

Cited by 15 publications

(17 citation statements)

References 51 publications

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“…Acetobacter pasteurianus , a member of Alphaproteobacteria , is an acetic acid-producing bacterium. Acetobacter pasteurianus is usually present in sugar-rich substrates such as fruits, flowers, and vegetables [ 33 ]. Clostridium papyrosolvens can produce a wide variety of carbohydrate-active enzymes to enhance cellulosic biomass degradation [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

Characterization of the Bacterial Community of Rumen in Dairy Cows with Laminitis

Guo

Mu²,

et al. 2021

Genes

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Laminitis is the inflammation of the lamella, and it has caused great economic loss to the dairy industry and attracted wide attention around the world. In recent years, microbiota are considered to play a significant role in various diseases processes. Therefore, our study aimed to explore the characteristics of ruminal microbiota in laminitis cows. The serum of bovines with or without laminitis was collected to detect concentrations of lipopolysaccharide (LPS), lactic acid, and histamine, and ruminal fluid was collected for 16S rDNA sequence analysis. The results showed a significant increase in LPS and lactic acid levels in the laminitis group compared to the control group cows. In addition, a higher abundance of Candidatus Saccharimonas, Saccharofermentans, Erysipelotrichaceae UCG-009 genus, Acetobacter pasteurianus, Clostridium papyrosolvens, Ruminococcaceae bacterium AE2021, Porphyromonas crevioricanis, Pseudomonas boreopolis, Pseudomonas psychrotolerans, Rothia nasimurium, and Rothia pickettii was detected in the rumen fluid of laminitis bovines. In conclusion, this article confirms that there are differences in rumen microbiota between healthy and laminitis bovines. The elevated abundance of bacteria that enrich acid-enhancing metabolites, as well as increase the concentration of lactic acid and LPS, could be harmful factors to bovines and increase the risk of laminitis.

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Section: Discussionmentioning

confidence: 99%

Characterization of the Bacterial Community of Rumen in Dairy Cows with Laminitis

Guo

Mu²,

et al. 2021

Genes

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“…The wide distribution observed for type II TA systems in these bacterial strains isolated from dairy products suggests an important role in the adaptation of these strains to environmental stresses, such as scarcity of nutrients typical of cheese. Recent studies have actually shown that the expression of DinJ/YafQ TA systems can be related to various environmental stresses, including those encountered in food production [16,41].…”

Section: Discussionmentioning

confidence: 99%

Genome Sequencing of five Lacticaseibacillus Strains and Analysis of Type I and II Toxin-Antitoxin System Distribution

et al. 2021

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The analysis of bacterial genomes is a potent tool to investigate the distribution of specific traits related to the ability of surviving in particular environments. Among the traits associated with the adaptation to hostile conditions, toxin–antitoxin (TA) systems have recently gained attention in lactic acid bacteria. In this work, genome sequences of Lacticaseibacillus strains of dairy origin were compared, focusing on the distribution of type I TA systems homologous to Lpt/RNAII and of the most common type II TA systems. A high number of TA systems have been identified spread in all the analyzed strains, with type I TA systems mainly located on plasmid DNA. The type II TA systems identified in these strains highlight the diversity of encoded toxins and antitoxins and their organization. This study opens future perspectives on the use of genomic data as a resource for the study of TA systems distribution and prevalence in microorganisms of industrial relevance.

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“…Bacteria have evolved sophisticated systems to sense and adapt to the dynamically changed environments. Two-component system (TCS), consisting of a sensory histidine kinase and a response regulator, is one kind of such systems involved in sensing environmental cues and regulating adaptive responses (Tiwari et al 2020 ; Xia et al 2020 ). In S. mutans , there is abundant evidence showing that the virulence factors production is co-regulated by diverse TCSs, and 15 TCSs have been found in the genome of S. mutans (Shanmugam et al 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…A genome-wide transcriptional response of S. mutans UA159 to TFs was investigated by RNA-sequencing (RNA-seq) analysis, which was performed as previously described (the detailed work flow was shown in ref. Xia et al 2020 ). Briefly, a 24-overnight culture of S. mutans UA159 was diluted by 1:100 into 100-mL fresh BHI medium containing 10 g/L sucrose and TFs with a final concentration of 0.5 mg/mL in 250-mL Erlenmeyer flasks.…”

Section: Methodsmentioning

confidence: 99%

Mechanistic insights into the inhibitory effect of theaflavins on virulence factors production in Streptococcus mutans

Kong

Xia

et al. 2021

AMB Expr

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Streptococcus mutans is the primary etiological agent associated with cariogenic process. The present study aimed to investigate the antibacterial and anti-virulence activities of theaflavins (TFs) to Streptococcus mutans UA159 as well as the underlying mechanisms. The results showed that TFs were capable of suppressing the acid production, cell adherence, water-insoluble exopolysaccharides production, and biofilm formation by S. mutans UA159 with a dosage-dependent manner while without influencing the cell growth. By a genome-wide transcriptome analysis (RNA-seq), we found that TFs attenuated the biofilm formation of S. mutans UA159 by inhibiting glucosyltransferases activity and the production of glucan-binding proteins (GbpB and GbpC) instead of directly blocking the expression of genes coding for glucosyltransferases. Further, TFs inhibited the expression of genes implicated in peptidoglycan synthesis, glycolysis, lipid synthesis, two-component system, signaling peptide transport (comA), oxidative stress response, and DNA replication and repair, suggesting that TFs suppressed the virulence factors of S. mutans UA159 by affecting the signal transduction and cell envelope stability, and weakening the ability of cells on oxidative stress resistance. In addition, an upregulated expression of the genes involved in protein biosynthesis, amino acid metabolism, and transport system upon TFs treatment indicated that cells increase the protein synthesis and nutrients uptake as one self-protective mechanism to cope with stress caused by TFs. The results of this study increase our current understanding of the anti-virulence activity of TFs on S. mutans and provide clues for the use of TFs in the prevention of dental caries.

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Transcriptome response of Acetobacter pasteurianus Ab3 to high acetic acid stress during vinegar production

Cited by 15 publications

References 51 publications

Characterization of the Bacterial Community of Rumen in Dairy Cows with Laminitis

Characterization of the Bacterial Community of Rumen in Dairy Cows with Laminitis

Genome Sequencing of five Lacticaseibacillus Strains and Analysis of Type I and II Toxin-Antitoxin System Distribution

Mechanistic insights into the inhibitory effect of theaflavins on virulence factors production in Streptococcus mutans

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