Transcriptome, proteome, and metabolome reveal the mechanism of tolerance to selenate toxicity in Cardamine violifolia

Rao, Shen; Tian, Yu; Cong, Xin; Lai, Xiaozhuo; Xiang, Jingjing; Cao, Jie; Liao, Xiaoli; Gou, Yuanyuan; Chao, Wei; Xue, Hua; Cheng, Shuiyuan; Xu, Feng

doi:10.1016/j.jhazmat.2020.124283

Cited by 46 publications

(21 citation statements)

References 73 publications

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“…High Se concentrations are phytotoxic as they cause non-specific, disruptive selenoamino acid incorporation into proteins ( Guignardi and Schiavon, 2017 ). Genes and proteins related to glutathione metabolism play important roles in assimilation and tolerance of Se in plants ( Chauhan et al, 2020 ; Rao et al, 2021 ). After long-term selenite treatment on tea seedlings, glutathione metabolism was differentially regulated ( Cao et al, 2018 ), remarkably, the expression of glutathione metabolism related genes and proteins were highly induced even with the short-term treatments of selenate and selenite in this study.…”

Section: Discussionmentioning

confidence: 99%

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Integrative Transcriptome and Proteome Analysis Reveals the Absorption and Metabolism of Selenium in Tea Plants [Camellia sinensis (L.) O. Kuntze]

Ren

Guo

et al. 2022

Front. Plant Sci.

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Certain tea plants (Camellia sinensis) have the ability to accumulate selenium. In plants, the predominant forms of bioavailable Se are selenite (SeO32–) and selenate (SeO42–). We applied transcriptomics and proteomics to hydroponically grown plants treated with selenite or selenate for 48 h in the attempt to elucidate the selenium absorption and assimilation mechanisms in tea. A total of 1,844 differentially expressed genes (DEGs) and 691 differentially expressed proteins (DEPs) were obtained by comparing the Na2SeO3 and Na2SeO4 treatments against the control. A GO analysis showed that the genes related to amino acid and protein metabolism and redox reaction were strongly upregulated in the plants under the Na2SeO3 treatment. A KEGG pathway analysis revealed that numerous genes involved in amino acid and glutathione metabolism were upregulated, genes and proteins associated with glutathione metabolism and ubiquinone and terpenoid-quinone biosynthesis were highly expressed. Genes participating in DNA and RNA metabolism were identified and proteins related to glutathione metabolism were detected in tea plants supplemented with Na2SeO4. ABC, nitrate and sugar transporter genes were differentially expressed in response to selenite and selenate. Phosphate transporter (PHT3;1a, PHT1;3b, and PHT1;8) and aquaporin (NIP2;1) genes were upregulated in the presence of selenite. Sulfate transporter (SULTR1;1 and SULTR2;1) expression increased in response to selenate exposure. The results of the present study have clarified Se absorption and metabolism in tea plants, and play an important theoretical reference significance for the breeding and cultivation of selenium-enriched tea varieties.

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Section: Discussionmentioning

confidence: 99%

“…Transcriptomics, metabolomics, and proteomics have been used to study Se disposition in living organisms ( Zhang C. et al, 2019 ; Chauhan et al, 2020 ; Rao et al, 2021 ). Accordingly, multiple omics technologies should also be applied to explore Se uptake and metabolism in tea plants.…”

Section: Introductionmentioning

confidence: 99%

Integrative Transcriptome and Proteome Analysis Reveals the Absorption and Metabolism of Selenium in Tea Plants [Camellia sinensis (L.) O. Kuntze]

Ren

Guo

et al. 2022

Front. Plant Sci.

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“…Se speciation was determined by liquid chromatography‐HG‐AFS (LC‐HG‐AFS) as reported by Rao et al 18 . Five Se standard compounds [SeCys 2 , SeMet, MeSeCys, Se(IV) and Se(VI)] were purchased from the National Institute of Metrology, China.…”

Section: Methodsmentioning

confidence: 99%

“…Se speciation was determined by liquid chromatography-HG-AFS (LC-HG-AFS) as reported by Rao et al 18 Five Se standard compounds [SeCys 2 , SeMet, MeSeCys, Se(IV) and Se(VI)] were purchased from the National Institute of Metrology, China. For the sample pretreatment, 0.20 g of dry sample was mixed with 10 mL of hydroxylamine hydrochloride in a 15-mL centrifuge tube and immersed in water at 37 °C for 1 h. The mixture was then centrifuged at 5000 × g for 10 min.…”

Section: Detection Of Total Se Se Speciation and Total Sulfur Contentsmentioning

confidence: 99%

Comparative study of the effects of selenium yeast and sodium selenite on selenium content and nutrient quality in broccoli florets (Brassica oleracea L. var. italica)

et al. 2021

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BACKGROUND: Approximately 0.5-1 billion people worldwide face the risk of selenium (Se) deficiency because of the low Se concentration in their diets. Broccoli can accumulate Se and comprises a source of daily Se supplement for humans. Se biofortification is an effective strategy for enhancing Se content in crops. In the present study, the effects of Se yeast and selenite application on the Se content and nutrient quality of broccoli were investigated. RESULTS: Broccoli growth was promoted by Se yeast but inhibited by selenite. The total Se content of broccoli florets remarkably increased with increasing exogenous Se fertilizer concentrations. The main Se species in broccoli florets were methylselenocysteine and selenomethionine, and their contents were significantly higher under Se yeast treatments than under selenite treatments. Se(VI) was detected only under selenite treatments. Se yeast and selenite had different influences on soluble sugar, soluble protein, vitamin C and free amino acid contents in broccoli florets. The total phenolic acid and glucosinolate contents were substantially increased by Se yeast and selenite, although the total flavonoid content was reduced by Se yeast. Tests on antioxidant enzyme activities revealed that several antioxidant enzymes (catalase, peroxidase, superoxide dismutase and glutathione peroxidase) responded to Se yeast and selenite treatments.CONCLUSION: Se yeast is preferred over selenite for maximizing Se uptake and nutrient accumulation in Se-rich broccoli cultivation. However, an extremely high Se content in broccoli florets cannot be directly consumed by humans, although they can be processed into Se supplements.

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“…Proteins were extracted according to Rao et al (2021). A BCA kit (Beyotime, Shanghai, China) was used to measure the protein concentrations, following the manufacturer's instructions.…”

Section: Quantitative Proteomic Data Analysismentioning

confidence: 99%

Multi-Approach Analysis Reveals Pathways of Cold Tolerance Divergence in Camellia japonica

Fan

Zhang

et al. 2022

Front. Plant Sci.

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Understanding the molecular mechanism of the cold response is critical to improve horticultural plant cold tolerance. Here, we documented the physiological, transcriptome, proteome, and hormonal dynamics to cold stress in temperate genotype (Tg) and subtropical genotype (Sg) populations of Camellia japonica. Tg C. japonica suffered minimal osmotic and oxidative damage compared to Sg C. japonica under the same cold treatment. Transcriptional and translational differences increased under the cold treatment, indicating that Tg C. japonica was affected by the environment and displayed both conserved and divergent mechanisms. About 60% of the genes responding to cold had similar dynamics in the two populations, but 1,896 transcripts and 455 proteins differentially accumulated in response to the cold between Tg and Sg C. japonica. Co-expression analysis showed that the ribosomal protein and genes related to photosynthesis were upregulated in Tg C. japonica, and tryptophan, phenylpropanoid, and flavonoid metabolism were regulated differently between the two populations under cold stress. The divergence of these genes reflected a difference in cold responsiveness. In addition, the decrease in the abscisic acid (ABA)/gibberellic acid (GA) ratio regulated by biosynthetic signal transduction pathway enhanced cold resistance in Tg C. japonica, suggesting that hormones may regulate the difference in cold responsiveness. These results provide a new understanding of the molecular mechanism of cold stress and will improve cold tolerance in horticultural plants.

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Transcriptome, proteome, and metabolome reveal the mechanism of tolerance to selenate toxicity in Cardamine violifolia

Cited by 46 publications

References 73 publications

Integrative Transcriptome and Proteome Analysis Reveals the Absorption and Metabolism of Selenium in Tea Plants [Camellia sinensis (L.) O. Kuntze]

Integrative Transcriptome and Proteome Analysis Reveals the Absorption and Metabolism of Selenium in Tea Plants [Camellia sinensis (L.) O. Kuntze]

Comparative study of the effects of selenium yeast and sodium selenite on selenium content and nutrient quality in broccoli florets (Brassica oleracea L. var. italica)

Multi-Approach Analysis Reveals Pathways of Cold Tolerance Divergence in Camellia japonica

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