Transcriptome profiling reveals the role of ZBTB38 knock-down in human neuroblastoma

Chen, Jie; Cheng, Xing; Li, Yan; Wang, Yabing; Wang, Haosen; Zhang, Zongmeng; Yu, Daolun; Li, Jie; Li, Honglin; Li, Jun; Cai, Yafei

doi:10.7717/peerj.6352

Cited by 8 publications

(14 citation statements)

References 57 publications

(61 reference statements)

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“…As a transcription factor, ZBTB38 is involved in cell regulation, proliferation and apoptosis, whereas functional de ciency of ZBTB38 induces the human neuroblastoma cell death [12]. Moreover, ZBTB38 play an oncogenic role in neuroblastoma and bladder cancer [12,13]. Based on these studies, we hypothesized that FAM83H-AS1 may be partially required for ZBTB38 to exert its oncogenic effect in ESCC.…”

Section: Discussionmentioning

confidence: 98%

“…and Bric a brac) domains [12]. As a transcription factor, ZBTB38 is involved in cell regulation, proliferation and apoptosis, whereas functional de ciency of ZBTB38 induces the human neuroblastoma cell death [12]. Moreover, ZBTB38 play an oncogenic role in neuroblastoma and bladder cancer [12,13].…”

Section: Discussionmentioning

confidence: 99%

“…However, the influence of miR-4684-5p on tumors has not been reported so far. It is noteworthy that as a transcription factor, ZBTB38 is involved in human neuroblastoma cell regulation, proliferation and apoptosis, whereas knockdown of ZBTB38 induces neuroblastoma cell death potentially [12]. ZBTB38 may promote cell migration and invasion of bladder cancer cells via up-modulation of Wnt/β-catenin pathway [13].…”

Section: Introductionmentioning

confidence: 99%

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LncRNA FAM83H-AS1 Promotes Aerobic Glycolysis and Tumor Progression by Regulating miR-4684-5p/ZBTB38 Axis in Esophageal Squamous Cell Carcinoma

Qian

Chen

et al. 2020

Preprint

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Background: Dysregulation of lncRNAs is implicated in esophageal squamous cell carcinoma (ESCC) progression; However, the precise function of lncRNA FAM83H-AS1 in ESCC remains unknown. Methods: FAM83H-AS1, miR-4684-5p and ZBTB38 mRNA expressions were detected via qRT-PCR. ZBTB38, GLUT1 and LDH-A protein expressions were tested via Western blot. Cell proliferation, migration and invasion were evaluated via CCK-8 and transwell assay, respectively. A nude mouse xenograft model was used to investigate the role of FAM83H-AS1 in xenograft ESCC growth. The metabolic shift in ESCC cells was examined via glycolysis analysis. The interaction between FAM83H-AS1, miR-4684-5p and ZBTB38 was analyzed via computational algorithms, RNA pull-down, RIP and dual luciferase reporter assay. Results: We found that FAM83H-AS1 was upmodulated in ESCC cell lines. FAM83H-AS1 knockdown hampered ESCC cell proliferation, migration, invasion and aerobic glycolysis, while FAM83H-AS1 overexpression demonstrated the opposite effects. FAM83H-AS1 knockdown also delayed the tumor growth in vivo. Moreover, FAM83H-AS1 interacted with miR-4684-5p/ZBTB38 axis in ESCC cells. ZBTB38 overexpression or miR-4684-5p inhibition partially reversed the inhibitory effect of FAM83H-AS1 knockdown on cell migration, invasion and aerobic glycolysis in ESCC cells. Conclusion: Our present results indicate FAM83H-AS1 accelerated aerobic glycolysis and tumorigenesis of ESCC by sponging miR-4684-5p and triggering the expression of ZBTB38, providing new insights into mechanism of ESCC progression and therapeutic strategy.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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LncRNA FAM83H-AS1 Promotes Aerobic Glycolysis and Tumor Progression by Regulating miR-4684-5p/ZBTB38 Axis in Esophageal Squamous Cell Carcinoma

Qian

Chen

et al. 2020

Preprint

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“…For instance, increasing ZBTB38 expression reduces apoptosis and promotes autophagy in a spinal cord injury model, and results in increased neuronal repair [25, 44, 45]. In contrast, ZBTB38 expression has been shown to promote proliferation and differentiation of a neuroblastoma cell line [46]. These differences in the functional roles of ZBTB38 may be attributed to subtype-specific sensitivity of neurons to oxidative stress [47].…”

Section: Discussionmentioning

confidence: 99%

ZBTB38 is dispensable for hematopoiesis and antibody responses

Wong

Bhattacharya

2020

Preprint

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14Members of the broad complex, tram track, bric-a-brac and zinc finger (BTB-ZF) 15 family of transcription factors, such as BCL-6, ZBTB20, and ZBTB32, regulate antigen-16 specific B cell differentiation, plasma cell longevity, and the duration of antibody 17 production. We found that ZBTB38, a different member of the BTB-ZF family that binds 18 methylated DNA at CpG motifs, is highly expressed by germinal center B cells and 19 plasma cells. To define the functional role of ZBTB38 in B cell responses, we generated 20 mice conditionally deficient in this transcription factor. Germinal center B cells lacking 21 ZBTB38 dysregulated very few genes relative to wild-type and heterozygous littermate 22 controls. Accordingly, mice with hematopoietic-specific deletion of Zbtb38 showed 23 normal germinal center B cell numbers and antibody responses following immunization 24 with hapten-protein conjugates. Memory B cells from these animals functioned normally 25 in secondary recall responses. Despite expression of ZBTB38 in hematopoietic stem 26 cells, progenitors and mature myeloid and lymphoid lineages were also present in 27 normal numbers in mutant mice. These data demonstrate that ZBTB38 is dispensable 28 for hematopoiesis and antibody responses. These conditional knockout mice may 29 instead be useful in defining the functional importance of ZBTB38 in other cell types and 30 contexts. 31 32 33 34 35 3 36 Introduction 37 Antibody responses following infections or vaccinations are initiated by a series 38 of B cell activation steps and fate decisions [1]. Upon recognition of cognate antigens 39 and other stimulatory signals, B cells grow in size, express a panel of activation 40 markers, begin to proliferate, and a subset undergoes immunoglobulin isotype-switching 41 [2]. In T cell-dependent responses, B cells then differentiate either into antibody-42 secreting plasma cells or into germinal center B cells. Germinal centers are the sites in 43 which somatic hypermutation and affinity maturation occur and are under substantial 44 replicative and DNA damage-induced stress. Germinal centers eventually produce long-45 lived plasma cells (LLPCs) and memory B cells (MBCs), which have distinct antigen 46 specificities and mediate different aspects of immunity [3]. LLPCs constitutively secrete 47 antibodies and are important for providing protection against re-infection by the same 48 pathogen. Memory B cells, on the other hand, can only provide protection after re-49 activation by a cognate antigen through rapid differentiation into plasma cells. Recent 50 studies have identified the broad complex, tram track, bric-a-brac and zinc finger (BTB-51 ZF) family of transcription factors as key regulators in B cell development. BTB-ZF 52 family members bind DNA through its C-terminal zinc finger domains and recruit SMRT 53 co-repressors and histone deacetylases to N-terminal BTB/POZ domains [4-8]. Family 54 members that regulate distinct aspects of B cell-mediated immunity include BCL-6, 55 ZBTB20, and ZBTB32. BCL-6 is important for ...

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“…For instance, increasing ZBTB38 expression reduces apoptosis and promotes autophagy in a spinal cord injury model, and results in increased neuronal repair [25,44,45]. In contrast, ZBTB38 expression has been shown to promote proliferation and differentiation of a neuroblastoma cell line [46]. These differences in the functional roles of ZBTB38 may be attributed to subtype-specific sensitivity of neurons to oxidative stress [47].…”

Section: Plos Onementioning

confidence: 99%

ZBTB38 is dispensable for antibody responses

Wong

Bhattacharya

2020

PLoS ONE

View full text Add to dashboard Cite

Members of the broad complex, tram track, bric-a-brac and zinc finger (BTB-ZF) family of transcription factors, such as BCL-6, ZBTB20, and ZBTB32, regulate antigen-specific B cell differentiation, plasma cell longevity, and the duration of antibody production. We found that ZBTB38, a different member of the BTB-ZF family that binds methylated DNA at CpG motifs, is highly expressed by germinal center B cells and plasma cells. To define the functional role of ZBTB38 in B cell responses, we generated mice conditionally deficient in this transcription factor. Germinal center B cells lacking ZBTB38 dysregulated very few genes relative to wild-type and heterozygous littermate controls. Accordingly, mice with hematopoietic-specific deletion of Zbtb38 showed normal germinal center B cell numbers and antibody responses following immunization with hapten-protein conjugates. Memory B cells from these animals functioned normally in secondary recall responses. Despite expression of ZBTB38 in hematopoietic stem cells, progenitors and mature myeloid and lymphoid lineages were also present in normal numbers in mutant mice. These data demonstrate that ZBTB38 is dispensable for hematopoiesis and antibody responses. These conditional knockout mice may instead be useful in defining the functional importance of ZBTB38 in other cell types and contexts.

show abstract

Transcriptome profiling reveals the role of ZBTB38 knock-down in human neuroblastoma

Cited by 8 publications

References 57 publications

LncRNA FAM83H-AS1 Promotes Aerobic Glycolysis and Tumor Progression by Regulating miR-4684-5p/ZBTB38 Axis in Esophageal Squamous Cell Carcinoma

LncRNA FAM83H-AS1 Promotes Aerobic Glycolysis and Tumor Progression by Regulating miR-4684-5p/ZBTB38 Axis in Esophageal Squamous Cell Carcinoma

ZBTB38 is dispensable for hematopoiesis and antibody responses

ZBTB38 is dispensable for antibody responses

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