Transcriptome profiling of two Dactylis glomerata L. cultivars with different tolerance in response to submergence stress

Zeng, Bing; Zhang, Yajie; Zhang, Ailing; Qiao, Dandan; Ren, Juncai; Li, Mingyang; Cai, Kaiyong; Zhang, Jinhua; Huang, Linkai

doi:10.1016/j.phytochem.2020.112378

Cited by 8 publications

(5 citation statements)

References 37 publications

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“…When the potato plantlets reached the five-leaf stage, the stems and leaves of the sensitive (4-0) and tolerant (4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19) varieties were treated with bentazone at concentrations of 1296 g a.i./ha, 648 g a.i./ha, and 324 g a.i./ha, at three replicates per concentration, with each replicate containing 50 seedlings. The varieties were examined for damage, and the survival rate was investigated on the fourth day after treatment.…”

Section: Potato Seedling Resistance To Bentazonementioning

confidence: 99%

“…Many studies have investigated gene expression to determine plant defense and stress mechanisms [12,13]. The transcriptional regulation of gene expression has been recognized as an important component of plant response, resulting in changes at the biochemical, cellular, and physiological levels [14,15]. Understanding the molecular mechanisms underlying herbicide resistance could facilitate the development of resistant crops, reducing the impact of weeds on production.…”

Section: Introductionmentioning

confidence: 99%

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RNA-Seq Transcriptome Analysis of Potato with Differential Tolerance to Bentazone Herbicide

et al. 2021

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Potato (Solanum tuberosum), an important food crop worldwide, is threatened by broadleaf weeds. Bentazone is an effective herbicide for controlling weeds; however, as a photosynthesis inhibitor, it can also affect potato plants. Therefore, screening potato seedlings for bentazone resistance and determining the genes involved is essential. Herein, we selected potato varieties with tolerance and sensitivity to bentazone. The photosynthetic rate of sensitive plants was notably affected by bentazone application, whereas the tolerant plants showed a significantly higher photosynthetic rate. We observed 95.7% bentazone degradation within 24 d after application in the tolerant plants. Transcriptome sequencing revealed that the numbers of differentially expressed genes (DEGs) between the tolerant and sensitive potato seedlings were 2703 and 11,024 before and after bentazone application, respectively. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed that the majority of DEGs were enriched in metabolic pathways, biosynthesis of secondary metals, carbon metabolism, glutathione metabolism, and photosynthesis. Polyphenol oxidase (PPO), flavonoid 3′,5′-methyltransferase-like (AOMT3), ribulose bisphosphate carboxylase small chain C (RBCS-C), and chalcone synthase 2 (CHS2) were identified as candidates contributing to bentazone tolerance. These results provide a theoretical basis for selecting potato stress-resistant resources in the future.

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Section: Potato Seedling Resistance To Bentazonementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

RNA-Seq Transcriptome Analysis of Potato with Differential Tolerance to Bentazone Herbicide

et al. 2021

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“…The expression pattern data of DgWRKY genes under different abiotic stresses and in varied tissues has been previously measured by our research team ( Huang et al, 2020 ; Huang et al, 2015 ; Ji et al, 2018 ; Zeng et al, 2020 ). This research has included expression profiles of orchardgrass under heat, drought, and submergence stress published by Huang et al (2015) , Ji et al (2018) , and Zeng et al (2020) , respectively. However, genome data for this species was not published until 2020, which required this previous work to be performed as unreferenced transcriptome analyses.…”

Section: Methodsmentioning

confidence: 99%

“…As such, this study aimed to identify and analyze the WRKY TF family in orchardgrass to provide a foundation for future molecular genetic improvement. Our research team has previously created high-quality expression profile data of orchardgrass under drought, heat, and submergence treatments ( Huang et al, 2015 ; Ji et al, 2018 ; Zeng et al, 2020 ). These data enable clarification of the mechanism by which WRKY TFs function in D. glomerata .…”

Section: Introductionmentioning

confidence: 99%

The whole-genome and expression profile analysis of WRKY and RGAs in Dactylis glomerata showed that DG6C02319.1 and DgWRKYs may cooperate in the immunity against rust

Ren

Zhang

et al. 2021

PeerJ

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Orchardgrass (Dactylis glomerata) is one of the top four perennial forages worldwide and, despite its large economic advantages, often threatened by various environmental stresses. WRKY transcription factors (TFs) can regulate a variety of plant processes, widely participate in plant responses to biotic and abiotic stresses, and are one of the largest gene families in plants. WRKYs can usually bind W-box elements specifically. In this study, we identified a total of 93 DgWRKY genes and 281 RGAs, including 65, 169 and 47 nucleotide-binding site-leucine-rich repeats (NBS-LRRs), leucine-rich repeats receptor-like protein kinases (LRR-RLKs), and leucine-rich repeats receptor-like proteins (LRR-RLPs), respectively. Through analyzing the expression of DgWRKY genes in orchardgrass under different environmental stresses, it was found that many DgWRKY genes were differentially expressed under heat, drought, submergence, and rust stress. In particular, it was found that the greatest number of genes were differentially expressed under rust infection. Consistently, GO and KEGG enrichment analysis of all genes showed that 78 DgWRKY TFs were identified in the plant–pathogen interaction pathway, with 59 of them differentially expressed. Through cis-acting element prediction, 154 RGAs were found to contain W-box elements. Among them, DG6C02319.1 (a member of the LRR-RLK family) was identified as likely to interact with 14 DGWRKYs. Moreover, their expression levels in susceptible plants after rust inoculation were first up-regulated and then down-regulated, while those in the resistant plants were always up-regulated. In general, DgWRKYs responded to both biotic stress and abiotic stress. DgWRKYs and RGAs may synergistically respond to the response of orchardgrass to rust. This study provides meaningful insight into the molecular mechanisms of WRKY proteins in orchardgrass.

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“…Studies on biotic and abiotic stress reports of orchardgrass and other cold-season forage grasses had mainly focused on drought, high temperature, and rust ( Huang et al, 2015 ; Ji et al, 2018 ; Yan et al, 2013 , 2016 ; Hu et al, 2014 ; Sun et al, 2020 ). Although there are some studies on submergence stress in orchardgrass ( Klaas et al., 2019 ; Qiao et al., 2020 ; Zeng et al., 2020 ), but it’s underlying submergence stress mechanisms in roots have not been reported. In the current study, the aim of this study was to understand the gene expression and underlying molecular mechanism of different submergence stress times response in two orchardgrass cultivars roots by RNA-seq.…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptomic analysis of the gene expression and underlying molecular mechanism of submergence stress response in orchardgrass roots

Zheng

et al. 2023

Front. Plant Sci.

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IntroductionSubmergence stress creates a hypoxic environment. Roots are the first plant organ to face these low-oxygen conditions, which causes damage and affects the plant growth and yield. Orchardgrass (Dactylis glomerata L.) is one of the most important cold-season forage grasses globally. However, their submergence stress-induced gene expression and the underlying molecular mechanisms of orchardgrass roots are still unknown.MethodsUsing the submergence-tolerant ‘Dianbei’ and submergence-sensitive ‘Anba’, the transcriptomic analysis of orchardgrass roots at different time points of submergence stress (0 h, 8 h, and 24 h) was performed.ResultsWe obtained 118.82Gb clean data by RNA-Seq. As compared with the control, a total of 6663 and 9857 differentially expressed genes (DEGs) were detected in Dianbei, while 7894 and 11215 DEGs were detected in Anba at 8 h and 24 h post-submergence-stress, respectively. Gene Ontology (GO) enrichment analysis obtained 986 terms, while Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis obtained 123 pathways. Among them, the DEGs in plant hormones, mitogen-activated protein kinase (MAPK) and Ca2+ signal transduction were significantly differentially expressed in Dianbei, but not in Anba.DiscussionThis study was the first to molecularly elucidate the submergence stress tolerance in the roots of two orchardgrass cultivars. These findings not only enhanced our understanding of the orchardgrass submergence tolerance, but also provided a theoretical basis 36 for the cultivation of submergence-tolerant forage varieties.

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Transcriptome profiling of two Dactylis glomerata L. cultivars with different tolerance in response to submergence stress

Cited by 8 publications

References 37 publications

RNA-Seq Transcriptome Analysis of Potato with Differential Tolerance to Bentazone Herbicide

RNA-Seq Transcriptome Analysis of Potato with Differential Tolerance to Bentazone Herbicide

The whole-genome and expression profile analysis of WRKY and RGAs in Dactylis glomerata showed that DG6C02319.1 and DgWRKYs may cooperate in the immunity against rust

Comparative transcriptomic analysis of the gene expression and underlying molecular mechanism of submergence stress response in orchardgrass roots

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