RNA-Seq Transcriptome Analysis of Potato with Differential Tolerance to Bentazone Herbicide

Abstract

Potato (Solanum tuberosum), an important food crop worldwide, is threatened by broadleaf weeds. Bentazone is an effective herbicide for controlling weeds; however, as a photosynthesis inhibitor, it can also affect potato plants. Therefore, screening potato seedlings for bentazone resistance and determining the genes involved is essential. Herein, we selected potato varieties with tolerance and sensitivity to bentazone. The photosynthetic rate of sensitive plants was notably affected by bentazone application, whereas the tolerant plants showed a significantly higher photosynthetic rate. We observed 95.7% bentazone degradation within 24 d after application in the tolerant plants. Transcriptome sequencing revealed that the numbers of differentially expressed genes (DEGs) between the tolerant and sensitive potato seedlings were 2703 and 11,024 before and after bentazone application, respectively. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed that the majority of DEGs were enriched in metabolic pathways, biosynthesis of secondary metals, carbon metabolism, glutathione metabolism, and photosynthesis. Polyphenol oxidase (PPO), flavonoid 3′,5′-methyltransferase-like (AOMT3), ribulose bisphosphate carboxylase small chain C (RBCS-C), and chalcone synthase 2 (CHS2) were identified as candidates contributing to bentazone tolerance. These results provide a theoretical basis for selecting potato stress-resistant resources in the future.

Multiple Metabolism Pathways of Bentazone Potentially Regulated by Metabolic Enzymes in Rice

“…We found that BNTZ exposure reduced the chlorophyll content in rice leaves, which was associated with impaired photosynthesis in rice and reduced production of plant energy and materials. Our result is consistent with the previous report on potato under BNTZ treatment . This result was also reinforced by the chlorophyll fluorescence parameters.…”

“…Our result is consistent with the previous report on potato under BNTZ treatment. 31 This result was also reinforced by the chlorophyll fluorescence parameters. The maximum photochemical efficiency (Fv/Fm) of PSII significantly decreased under BNTZ stress, indicating that the PSII reaction centers was impaired.…”

Genetic Dissection of Bentazone Tolerance Loci in Cultivated Soybeans: A Genome-Wide Association Study

“…Bentazone [3-Isopropyl-1H-2,1,3-benzothiadiazin-4(3H)-one 2,2-dioxide] was first documented in 1975. Bentazone is a POST herbicide used to control broadleaf weeds in the cultivation of cereals, legumes [6], vegetables [17], and tuber crops [18]. Bentazone is a contact herbicide that inhibits the capacity of vulnerable plants to use sunlight in photosynthesis to produce the energy necessary for survival.…”

​Effect of Legume and Cereal Mulches on Weed Dynamics and Potato Yield

“…Weed control strategies in crop production can be divided into chemical and nonchemical practices (Zangoueinejad and Kazemeini, 2014;Azadbakht et al, 2017;Zangoueinejad et al, 2018;Zangoueinejad, 2019;El-Metwally and El-Wakeel, 2019;Nabati Souha et al, 2020a,b;Zangoueinejad et al, 2021). Although various herbicide options are available to control weed abundances in crop fields worldwide (Alebrahim et al, 2011;Khatami Kalkhoran et al, 2017;Zangoueinej ad et al, 2019;Alebrahim et al, 2021;Guo et al, 2021). However, cover crops can be considered an effective weed control strategy in sustainable agriculture by reducing the side effects of herbicide use, improving soil quality over time and increasing crop yield production (Barbas et al, 2020).Therefore, due to the destructive effects of herbicides on natural resources, concerns have been raised in recent years (Zangoueinejad andGhadiri 2014, 2016;Mehdizadeh et al, 2017;Alebrahim et al, 2017;Hashemi et al, 2021;Camargo et al, 2020;Zangoueinejad and Alebrahim, 2021b) leading to an increased attention on their negative effects on the ecosystems, including soil and water pollution and its phytotoxic effects on crops in rotation (Mehdizadeh, 2016;Azadbakht et al, 2019;Khatami Kalkhoran et al, 2017;Zangoueinejad and Alebrahim, 2021a).…”